壬基酚对斑马鱼不同组织中抗氧化酶活力的影响
Effects of Nonylphenol on Activities of Antioxidant Enzymes in Different Tissues of Zebrafish
-
摘要: 为研究壬基酚(nonylphenol, NP)对鱼类体内抗氧化酶活力的动态影响，探究NP对鱼类的毒性效应机制，以斑马鱼为受试生物，采用半静态实验法测定NP对斑马鱼的急性半致死浓度(LC50)，并以不同浓度(5、50和100 μg·L-1)的NP进行14 d暴露和7 d净化实验，探究NP对头部、肌肉和内脏团中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽S-转移酶(GST)活力和还原型谷胱甘肽(GSH)含量的影响。结果表明，NP对斑马鱼的24～96 h的LC50为487.6～273.6 μg·L-1。NP胁迫下斑马鱼头部SOD活力在5 μg·L-1组表现出显著的毒性兴奋效应；肌肉和内脏团的SOD活力在暴露中后期受到诱导，尤其在高浓度胁迫下更快产生该效应。头部和肌肉的CAT活力分别受NP的抑制和诱导作用，但均随时间增加先上升后下降；而CAT在内脏团中的活力受明显抑制后逐渐上升，100 μg·L-1组受到的抑制作用更强且更持久。抗氧化酶受NP影响的差异可能与抗氧化酶在各组织内的敏感性和赋存含量密切相关。50 μg·L-1和100 μg·L-1组内脏团的GST活力随时间延长而增强，并且其平衡水平与NP浓度呈正相关。GSH在头部和肌肉内的含量在初期被NP大量消耗后，逐渐通过生物合成而增加。净化阶段，高浓度NP胁迫后的抗氧化酶活力一般恢复较慢。本研究结果将为NP对水生动物的毒性风险评价提供科学依据。Abstract: In order to study the dynamic effects of nonylphenol (NP) on the activities of antioxidant enzymes and explore the toxicity of NP to fish, we selected zebrafish (Danio rerio) as the experimental organism, investigated the acute median lethal concentration (LC50) of NP via a semi-static toxicity test, and probed the impacts of NP on activities of superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and glutathione (GSH) content in zebrafish (head, muscle, and visceral mass) during the 14-d exposure and 7-d purification test at different concentrations (5, 50, 100 μg·L-1) of NP. The results showed that the 24-h～96-h LC50 values were 487.6～273.6 μg·L-1. In the head, a significant toxicity effect on SOD activity was found in the 5 μg·L-1 treatment at the early exposure stage. At the middle and later stages, the SOD activities in muscle and visceral mass were induced especially at a high concentration of NP. Moreover, NP continuously inhibited the CAT activity in head while the activity in muscle was induced at the middle stage. Both of the CAT activities in head and muscle were increased and then decreased over time. As for the visceral mass, the CAT activity was significantly inhibited and then gradually increased, and 100 μg·L-1 of NP caused more severe and durable inhibition. The effects of NP on the activities of antioxidant enzymes were different due to their varying sensitivities and contents in the three tissues. In the 50 μg·L-1 and 100 μg·L-1 of NP-treated groups, the GST activity in visceral mass was increased over time, and the equilibrium activity was positively correlated with the NP concentration. After consuming largely by NP at the early stage of exposure, the total GSH contents in head and muscle were increased over time subsequently through biosynthesis. The enzyme activities basically indicated a slower recovery after exposure to a higher level of NP at the purification stage. Our findings would provide essential information for interpreting the ecological risks of NP in the aquatic ecosystem.
-
Key words:
- nonylphenol /
- zebrafish /
- acute toxicity /
- antioxidant enzyme /
- sensitive indicator
-
-
Ying G G, Williams B, Kookana R. Environmental fate of alkylphenols and alkylphenol ethoxylates: A review [J]. Environment International, 2002, 28(3): 215-226 Servos M R. Review of the aquatic toxicity, estrogenic responses and bioaccumulation of alkylphenols and alkylphenol polyethoxylates [J]. Water Quality Research Journal, 1999, 34(1): 123-178 Shirdel I, Kalbassi M R, Esmaeilbeigi M, et al. Disruptive effects of nonylphenol on reproductive hormones, antioxidant enzymes, and histology of liver, kidney and gonads in Caspian trout smolts [J]. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2020, 232: 108756 Rastgar S, Movahedinia A, Salamat N, et al. Interruption of immune responses in primary macrophages exposed to nonylphenol provides insights into the role of ER and NF-KB in immunotoxicity of Persian sturgeon [J]. Fish & Shellfish Immunology, 2019, 86: 125-134 Madigou T, Goff P L, Salbert G, et al. Effects of nonylphenol on estrogen receptor conformation, transcriptional activity and sexual reversion in rainbow trout (Oncorhynchus mykiss) [J]. Aquatic Toxicology, 2001, 53(3-4): 173-186 Xia J G, Niu C J, Pei X J. Effects of chronic exposure to nonylphenol on locomotor activity and social behavior in zebrafish (Danio rerio) [J]. Journal of Environmental Sciences, 2010, 22(9): 1435-1440 Puy-Azurmendi E, Olivares A, Vallejo A, et al. Estrogenic effects of nonylphenol and octylphenol isomers in vitro by recombinant yeast assay (RYA) and in vivo with early life stages of zebrafish [J]. Science of the Total Environment, 2014, 466-467: 1-10 Sun D, Chen Q, Zhu B, et al. Multigenerational reproduction and developmental toxicity, and HPG axis gene expression study on environmentally-relevant concentrations of nonylphenol in zebrafish [J]. Science of the Total Environment, 2021, 764: 144259 Fan J J, Wang S, Tang J P, et al. Bioaccumulation of endocrine disrupting compounds in fish with different feeding habits along the largest subtropical river, China [J]. Environmental Pollution, 2019, 247: 999-1008 Chen R, Yin P H, Zhao L, et al. Spatial-temporal distribution and potential ecological risk assessment of nonylphenol and octylphenol in riverine outlets of Pearl River Delta, China [J]. Journal of Environmental Sciences, 2014, 26(11): 2340-2347 褚春莹, 但丽霞, 蒋海威, 等. 胶州湾入海河流和排污口水体中壬基酚的污染状况调查及入海通量核算[J]. 中国环境监测, 2013, 29(2): 29-33 Chu C Y, Dan L X, Jiang H W, et al. Pollution status and flux of nonylphenol in the water of the rivers and drainage outlet entering the Jiaozhou Bay [J]. Environmental Monitoring in China, 2013, 29(2): 29-33 (in Chinese)
Lietti E, Marin M G, Matozzo V, et al. Uptake and elimination of 4-nonylphenol by the clam Tapes philippinarum [J]. Archives of Environmental Contamination and Toxicology, 2007, 53(4): 571-578 Vidal-Liñán L, Bellas J, Salgueiro-González N, et al. Bioaccumulation of 4-nonylphenol and effects on biomarkers, acetylcholinesterase, glutathione-S-transferase and glutathione peroxidase, in Mytilus galloprovincialis mussel gills [J]. Environmental Pollution, 2015, 200: 133-139 Nair P M G, Park S Y, Choi J. Expression of catalase and glutathione S-transferase genes in Chironomus riparius on exposure to cadmium and nonylphenol [J]. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2011, 154(4): 399-408 Mansour S A, Mossa A T H. Lipid peroxidation and oxidative stress in rat erythrocytes induced by chlorpyrifos and the protective effect of zinc [J]. Pesticide Biochemistry and Physiology, 2009, 93(1): 34-39 Bal A, Panda F, Pati S G, et al. Modulation of physiological oxidative stress and antioxidant status by abiotic factors especially salinity in aquatic organisms [J]. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2021, 241: 108971 Chen S Y, Shen Z Y, Ding J W, et al. Sulfamethoxazole induced systematic and tissue-specific antioxidant defense in marine mussels (Mytilus galloprovincialis): Implication of antibiotic’s ecotoxicity [J]. Chemosphere, 2021, 279: 130634 李翠萍, 吴民耀, 王宏元. 3种半数致死浓度计算方法之比较[J]. 动物医学进展, 2012, 33(9): 89-92 Li C P, Wu M Y, Wang H Y. LC50 caculated by Kochi, probit analysis and linear regression methods [J]. Progress in Veterinary Medicine, 2012, 33(9): 89-92 (in Chinese)
Mihaljevič I, Bašica B, Marakovič N, et al. Interaction of organotin compounds with three major glutathione S-transferases in zebrafish [J]. Toxicology in Vitro, 2020, 62: 104713 Arnot J A, Gobas F A. A review of bioconcentration factor (BCF) and bioaccumulation factor (BAF) assessments for organic chemicals in aquatic organisms [J]. Environmental Reviews, 2006, 14(4): 257-297 Bhattacharya H, Xiao Q, Lun L M. Toxicity studies of nonylphenol on rosy barb (Puntius conchonious): A biochemical and histopathological evaluation [J]. Tissue and Cell, 2008, 40(4): 243-249 陈建华, 谢艳颖, 陈世红, 等. 茶多酚对壬基酚所致斑马鱼急性死亡和遗传损伤的保护作用[J]. 环境污染与防治, 2018, 40(10): 1126-1131 Chen J H, Xie Y Y, Chen S H, et al. The protection of Danio rerio from acute lethal toxicity and genetic damage induced by nonylphenol through tea polyphenol [J]. Environmental Pollution & Control, 2018, 40(10): 1126-1131 (in Chinese)
张静. 环境激素对斑马鱼免疫相关基因表达的影响[D]. 济南: 山东师范大学, 2012: 31 Zhang J. Effects of environmental hormones on the expression of immune-related genes of the zebrafish (Danio rerio) [D]. Ji’nan: Shandong Normal University, 2012: 31 (in Chinese) 戚珍珠, 雷忻, 王文强, 等. 壬基酚胁迫对幼龄泥鳅雌激素活性及抗氧化酶活力的影响[J]. 西北农林科技大学学报: 自然科学版, 2017, 45(2): 49-55 Qi Z Z, Lei X, Wang W Q, et al. Effect of nonylphenol expose on estrogenic activity and antioxidant enzymes of juvenile Misgurnus anguillicaudatus [J]. Journal of Northwest A & F University: Natural Science Edition, 2017, 45(2): 49-55 (in Chinese)
高永刚, 李正炎. 壬基酚对栉孔扇贝组织抗氧化酶活性的影响[J]. 中国海洋大学学报: 自然科学版, 2006, 36(S1): 135-138 Gao Y G, Li Z Y. Effect of nonylphenol on anti-oxidant enzymes of Chlamys farreri [J]. Periodical of Ocean University of China, 2006, 36(S1): 135-138 (in Chinese)
巩秀玉, 黄志斐, 王贺威, 等. 壬基酚对波纹巴非蛤(Paphia undulata)外套膜毒性效应研究[J]. 海洋环境科学, 2015, 34(2): 234-239 Gong X Y, Huang Z F, Wang H W, et al. Toxic effect of nonylphenol (NP) on the mantle of Paphia undulate [J]. Marine Environmental Science, 2015, 34(2): 234-239 (in Chinese)
Orias F, Simon L, Mialdea G, et al. Bioconcentration of 15N-tamoxifen at environmental concentration in liver, gonad and muscle of Danio rerio [J]. Ecotoxicology and Environmental Safety, 2015, 120: 457-462 巩秀玉, 陈海刚, 张喆, 等. 壬基酚对波纹巴非蛤(Paphia undulata)内脏团毒性效应[J]. 生态学杂志, 2012, 31(6): 1473-1479 Gong X Y, Chen H G, Zhang Z, et al. Toxic effect of nonylphenol on Paphia undulata visceral mass [J]. Chinese Journal of Ecology, 2012, 31(6): 1473-1479 (in Chinese)
Lushchak V I. Environmentally induced oxidative stress in aquatic animals [J]. Aquatic Toxicology, 2011, 101(1): 13-30 Hughes E M, Gallagher E P. Effects of 17-beta estradiol and 4-nonylphenol on phase Ⅱ electrophilic detoxification pathways in largemouth bass (Micropterus salmoides) liver [J]. Comparative Biochemistry and Physiology Toxicology & Pharmacology, 2004, 137(3): 237-247 张海丽, 边海燕, 杨跃志, 等. 酚类污染物对菲律宾蛤仔抗氧化和解毒系统相关酶活性的影响[J]. 中国海洋大学学报: 自然科学版, 2012, 42(3): 21-26 Zhang H L, Bian H Y, Yang Y Z, et al. Effects of phenolic compounds on enzyme activities of antioxidant and detoxification systems in Ruditapes philippinarum [J]. Periodical of Ocean University of China, 2012, 42(3): 21-26 (in Chinese)
杨琦, 张金补, 董然然. 环境激素壬基酚对杂交鲟的氧化胁迫作用[J]. 现代农业科技, 2018(2): 240-242 Yang Q, Zhang J B, Dong R R. Effect of nonylphenol on oxidative stress of hybrid sturgeon [J]. Modern Agricultural Science and Technology, 2018 (2): 240-242 (in Chinese)
-
点击查看大图
计量
- 文章访问数: 2292
- HTML全文浏览数: 2292
- PDF下载数: 48
- 施引文献: 0
下载:
