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复合菌对黑臭水体中S2-的氧化条件优化及动力学特性

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徐瑶瑶, 宋晨, 宋楠楠, 王进, 岳正波, 刘晓玲. 复合菌对黑臭水体中S2-的氧化条件优化及动力学特性[J]. 环境工程学报, 2019, 13(3): 530-540. doi: 10.12030/j.cjee.201901006
引用本文: 徐瑶瑶, 宋晨, 宋楠楠, 王进, 岳正波, 刘晓玲. 复合菌对黑臭水体中S2-的氧化条件优化及动力学特性[J]. 环境工程学报, 2019, 13(3): 530-540. doi: 10.12030/j.cjee.201901006
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XU Yaoyao, SONG Chen, SONG Nannan, WANG Jin, YUE Zhengbo, LIU Xiaoling. Condition optimization and kinetic characteristics of S2- bio-oxidation in a black-stinking water body by composite microorganisms[J]. Chinese Journal of Environmental Engineering, 2019, 13(3): 530-540. doi: 10.12030/j.cjee.201901006
Citation: XU Yaoyao, SONG Chen, SONG Nannan, WANG Jin, YUE Zhengbo, LIU Xiaoling. Condition optimization and kinetic characteristics of S2- bio-oxidation in a black-stinking water body by composite microorganisms[J]. Chinese Journal of Environmental Engineering, 2019, 13(3): 530-540. doi: 10.12030/j.cjee.201901006
shu

复合菌对黑臭水体中S2-的氧化条件优化及动力学特性

  • 1.

    合肥工业大学资源与环境工程学院，合肥 230009

  • 2.

    中国环境科学研究院，流域水环境污染综合治理研究中心，北京 100012

  • 3.

    南京瑞迪建设科技有限公司，南京 210029

  • 基金项目:

    北京市自然科学基金面上项目8182058

    中央级公益性科研院所基本科研业务费专项JY-201209012北京市自然科学基金面上项目(8182058)

    中央级公益性科研院所基本科研业务费专项(JY-201209012)

Condition optimization and kinetic characteristics of S2- bio-oxidation in a black-stinking water body by composite microorganisms

  • 1.

    School of Resources and Environmental Engineering，Hefei University of Technology，Hefei 230009，China

  • 2.

    Research Center for Comprehensive Treatment of Water Environmental Pollution in River Basin, Chinese Research Academy of Environmental Sciences, Beijing 100012, China

  • 3.

    Nanjing R&D Tech Group Co.Ltd.，Nanjing 210029，China

  • Fund Project:

  • 摘要: 以S2-的氧化率为主要考察指标，对从北京黑臭水体东沙河筛选获得的3株高效S2-氧化土著微生物sp1(Citrobacter sp.)、sp2(Ochrobactrum sp.)和sp3(Stenotrophomonas sp.)进行复配，获得硫氧化复合菌(sulphur oxidizing composite microorganisms, SOCM)，比较单株菌sp1、sp2和sp3和SOCM对S2-的氧化效果。结果表明，SOCM对S2-的氧化能力明显优于单菌株。SOCM在复配比例为1∶1∶1，温度为25 ℃，初始pH为7时对北京市东沙河黑臭水样中S2-的氧化效果最好，氧化率最高达到76.7%；同时，色度、COD、NH3-N和TP的去除率可分别达到83.3%、69.2%、77.9%和68.2%。此外，建立了SOCM氧化S2-的动力学方程。当SOCM初始菌浓度从0.01 g·L-1逐渐提高到10 g·L-1时，底物比氧化速率常数Km随之减小，S2-平均氧化速率提高。但是，当SOCM初始菌浓度从10 g·L-1逐渐提高到50 g·L-1时，S2-平均氧化速率不再随着初始菌浓度的升高而加快。最后，将SOCM接种于北京清河和景观沟渠黑臭水样中，其对S2-氧化率分别达到67.0%和64.1%；同时，色度亦分别下降了83.3%和79.2%。研究为黑臭水体的微生物法治理提供了参考。
    Abstract: In this study, S2- oxidation ratios was taken as the main index, three indigenous pure strains of sp1 (Citrobacter sp.), sp2 (Ochrobactrum sp.) and sp3 (Stenotrophomonas sp.) with high efficiently S2- oxidation were isolated from Dongsha river, a black-stinking water body in Beijing. They were mixed to produce the sulfur-oxidizing composite microorganisms(SOCM).The S2- oxidation efficiencies of sp1, sp2, sp3 and SOCM were compared. The results showed that SOCM had an obviously better performance on S2- oxidation than these three pure bacteria, and the best S2- oxidation effect in the black-stinking water samples from Dongsha river in Beijing occurred at the complex ratio for SOCM production 1∶1∶1, 25 ℃ and the initial pH of 7, and the highest S2- oxidation efficiency reached 76.7%, and the removal efficiencies of chroma, COD, NH3-N and TP were 83.3%, 69.2%, 77.9% and 68.2%, respectively. In addition, the kinetic equation for S2- oxidation by SOCM was determined. When the initial bacterial concentration of SOCM increased from 0.01 g·L-1 to 10 g·L-1, the substrate specific oxidation rate constant Km decreased, and the average S2- oxidation rate increased. When the initial bacterial concentration of SOCM increased from 10 g·L-1 to 50 g·L-1, the average S2- oxidation ratio did not increase any more. Finally, after inoculating SOCM into the black-stinking water samples from Qinghe river and Jingguangouqu river in Beijing, the S2- oxidation efficiencies reached 67.0% and 64.1%, and their chroma values decreased by 83.3% and 79.2%, respectively. This study provided a reference for the microbial remediation of black-stinking water body.
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  • 刊出日期:  2019-03-14

复合菌对黑臭水体中S2-的氧化条件优化及动力学特性

  • 1. 合肥工业大学资源与环境工程学院，合肥 230009
  • 2. 中国环境科学研究院，流域水环境污染综合治理研究中心，北京 100012
  • 3. 南京瑞迪建设科技有限公司，南京 210029
基金项目:

北京市自然科学基金面上项目8182058

中央级公益性科研院所基本科研业务费专项JY-201209012北京市自然科学基金面上项目(8182058)

中央级公益性科研院所基本科研业务费专项(JY-201209012)

摘要: 以S2-的氧化率为主要考察指标，对从北京黑臭水体东沙河筛选获得的3株高效S2-氧化土著微生物sp1(Citrobacter sp.)、sp2(Ochrobactrum sp.)和sp3(Stenotrophomonas sp.)进行复配，获得硫氧化复合菌(sulphur oxidizing composite microorganisms, SOCM)，比较单株菌sp1、sp2和sp3和SOCM对S2-的氧化效果。结果表明，SOCM对S2-的氧化能力明显优于单菌株。SOCM在复配比例为1∶1∶1，温度为25 ℃，初始pH为7时对北京市东沙河黑臭水样中S2-的氧化效果最好，氧化率最高达到76.7%；同时，色度、COD、NH3-N和TP的去除率可分别达到83.3%、69.2%、77.9%和68.2%。此外，建立了SOCM氧化S2-的动力学方程。当SOCM初始菌浓度从0.01 g·L-1逐渐提高到10 g·L-1时，底物比氧化速率常数Km随之减小，S2-平均氧化速率提高。但是，当SOCM初始菌浓度从10 g·L-1逐渐提高到50 g·L-1时，S2-平均氧化速率不再随着初始菌浓度的升高而加快。最后，将SOCM接种于北京清河和景观沟渠黑臭水样中，其对S2-氧化率分别达到67.0%和64.1%；同时，色度亦分别下降了83.3%和79.2%。研究为黑臭水体的微生物法治理提供了参考。

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