佳乐麝香降解菌的筛选、鉴定及降解特性研究
Screening, Identification and Degradation Characteristics of Galaxolide Degrading Bacteria
-
摘要: 佳乐麝香(HHCB)为药品与个人护理品(PPCPs)类新型污染物之一，因其特有的持久性和亲脂性，在环境介质中广泛积累，造成了一定程度的环境污染。为探究人工合成麝香的微生物修复机制，采用富集、分离、驯化的方法，从受污染的土壤样品筛选到一株能利用HHCB生长并具有较好降解能力的菌株H10。根据菌种形态特征、生理生化分析及16S rRNA序列比对结果，初步鉴定其为金黄杆菌属(Chryseobacterium sp.)细菌。该菌降解HHCB的最适宜降解条件为：接种量5%、pH 7.0、温度30 ℃，72 h后对200 mg·L-1的HHCB降解率为68.07%。响应面实验结果显示：当接种量为5.95%，pH为7.4，温度为30.55 ℃、底物浓度为200 mg·L-1时，72 h后菌体密度可达0.662，优化后菌株在此条件下对HHCB的降解率为70.15%。研究发现菌株H10具有降解HHCB的能力，不仅丰富了HHCB降解菌库，也为受人工合成麝香污染环境的微生物修复提供了理论基础。Abstract: The synthetic musk galaxolide (HHCB) is a new pollutant among pharmaceuticals and personal care products (PPCPs), which accumulates widely in environmental media due to its unique persistence and lipophilicity, resulting in significant environmental pollution. In order to investigate the microbial remediation mechanism of synthetic musk, a strain H10 was screened from contaminated soil samples by enrichment, isolation, and domestication methods. It exhibited excellent degradation ability towards HHCB. The strain H10 was preliminarily identified as the Chryseobacterium sp., based on morphological characteristics, physiological and biochemical analysis, as well as 16S rRNA sequence comparison. The most suitable optimal conditions for HHCB degradation were determined as follows: inoculation amount of 5%, pH 7.0, temperature of 30 ℃. After 72 h of incubation under these conditions, the degradation rate of HHCB at a concentration of 200 mg·L-1 reached 68.07%. The results of the response surface experiment indicated that when the inoculation amount was adjusted to 5.95%, pH to 7.4, temperature to 30.55 ℃ and substrate concentration remained at 200 mg·L-1 after optimization for bacterial density reached up to 0.662 after incubation for another period of time while achieving a degradation rate of HHCB at approximately 70.15%. The study found that the strain H10 had the ability to degrade HHCB, which not only enriches the HHCB degrading bacterial library, but also provides a theoretical basis for microbial remediation of environments contaminated with artificially synthesized musk.
-
Key words:
- galaxolide (HHCB) /
- Chryseobacterium /
- degradation rate /
- microbial degradation
-
-
Lyu Y, Ren S, Zhong F Y, et al. Synthetic musk fragrances in sediments from a subtropical river-lake system in Eastern China: Occurrences, profiles, and ecological risks[J]. Environmental Science and Pollution Research International, 2021, 28(12): 14597-14606 Kannan K, Reiner J L, Yun S H, et al. Polycyclic musk compounds in higher trophic level aquatic organisms and humans from the United States[J]. Chemosphere, 2005, 61(5): 693-700 Sumner N R, Guitart C, Fuentes G, et al. Inputs and distributions of synthetic musk fragrances in an estuarine and coastal environment; a case study[J]. Environmental Pollution, 2010, 158(1): 215-222 Clara M, Gans O, Windhofer G, et al. Occurrence of polycyclic musks in wastewater and receiving water bodies and fate during wastewater treatment[J]. Chemosphere, 2011, 82(8): 1116-1123 Merian E. A review of: “the handbook of environmental chemistry”[J]. International Journal of Environmental Analytical Chemistry, 1981, 10(1): 83-84 Fromme H, Otto T, Pilz K. Polycyclic musk fragrances in different environmental compartments in Berlin (Germany)[J]. Water Research, 2001, 35(1): 121-128 Wang T, Zou H Y, Li D Y, et al. Global distribution and ecological risk assessment of synthetic musks in the environment[J]. Environmental Pollution, 2023, 331(Pt 2): 121893 Zeng X Y, Sheng G Y, Xiong Y, et al. Determination of polycyclic musks in sewage sludge from Guangdong, China using GC-EI-MS[J]. Chemosphere, 2005, 60(6): 817-823 Zhang Q R, Wang J M, Lyu H H, et al. Ball-milled biochar for galaxolide removal: Sorption performance and governing mechanisms[J]. Science of the Total Environment, 2019, 659: 1537-1545 王凤花, 宋鑫, 曹建峰, 等. 污水处理厂进出水及其受纳水体中典型PPCPs的污染特征[J]. 中国环境监测, 2014, 30(4): 112-117 Wang F H, Song X, Cao J F, et al. Occurrence of typical PPCPs in municipal wastewater treatment plants and the receiving water[J]. Environmental Monitoring in China, 2014, 30(4): 112-117(in Chinese)
Wanda E M M, Nyoni H, Mamba B B, et al. Application of silica and germanium dioxide nanoparticles/polyethersulfone blend membranes for removal of emerging micropollutants from water[J]. Physics and Chemistry of the Earth, Parts A/B/C, 2018, 108: 28-47 Lee P H, Ong S K, Golchin J, et al. Use of solvents to enhance PAH biodegradation of coal tar-contaminated soils[J]. Water Research, 2001, 35(16): 3941-3949 Zhang B W, Guo Y, Huo J Y, et al. Combining chemical oxidation and bioremediation for petroleum polluted soil remediation by BC-nZVI activated persulfate[J]. Chemical Engineering Journal, 2020, 382: 123055 Madnay M M Y, Obaid W A, Selim S, et al. Rhodospirillum sp. JY3: An innovative tool to mitigate the phytotoxic impact of galaxolide on wheat (Triticum aestivum) and faba bean (Vicia faba) plants[J]. Frontiers in Plant Science, 2022, 13: 1037474 张珣, 李晓云, 孙丽娜. 污泥堆制发酵过程中多环麝香的降解及微生物群落的演替[J]. 沈阳农业大学学报, 2015, 46(1): 96-100 Zhang X, Li X Y, Sun L N. Polycyclic musk degradation and succession of microbial community during sludge composting[J]. Journal of Shenyang Agricultural University, 2015, 46(1): 96-100(in Chinese)
Martin C, Moeder M, Daniel X, et al. Biotransformation of the polycyclic musks HHCB and AHTN and metabolite formation by fungi occurring in freshwater environments[J]. Environmental Science & Technology, 2007, 41(15): 5395-5402 顾平, 周启星, 王鑫, 等. 一株土著B[a]P降解菌的筛选及降解特性研究[J]. 农业环境科学学报, 2018, 37(5): 926-932 Gu P, Zhou Q X, Wang X, et al. The screening-out and degradation character of an indigenous benzo[a]pyrene-degrading bacteria[J]. Journal of Agro-Environment Science, 2018, 37(5): 926-932(in Chinese)
罗庆, 孙丽娜. 微波辅助萃取气相色谱质谱法测定土壤、底泥及植物样品中的多环麝香[J]. 分析试验室, 2011, 30(4): 50-53 Luo Q, Sun L N. Determination of polycyclic musks in soil, sediment and plants using microwave-assisted solvent extraction with gas chromatography-mass spectrometry[J]. Chinese Journal of Analysis Laboratory, 2011, 30(4): 50-53(in Chinese)
东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京: 科学出版社, 2001: 349-388 Ishii S, Suzuki S, Yamanaka Y, et al. Population dynamics of electrogenic microbial communities in microbial fuel cells started with three different inoculum sources[J]. Bioelectrochemistry, 2017, 117: 74-82 Singh B K, Walker A. Microbial degradation of organophosphorus compounds[J]. FEMS Microbiology Reviews, 2006, 30(3): 428-471 Chodak M, Gołębiewski M, Morawska-Płoskonka J, et al. Diversity of microorganisms from forest soils differently polluted with heavy metals[J]. Applied Soil Ecology, 2013, 64: 7-14 Parolini M, Magni S, Traversi I, et al. Environmentally relevant concentrations of galaxolide (HHCB) and tonalide (AHTN) induced oxidative and genetic damage in Dreissena polymorpha[J]. Journal of Hazardous Materials, 2015, 285: 1-10 周启星, 王美娥, 范飞, 等. 人工合成麝香的环境污染、生态行为与毒理效应研究进展[J]. 环境科学学报, 2008, 28(1): 1-11 Zhou Q X, Wang M E, Fan F, et al. Research progress in environmental pollution, ecological behavior and toxicological effects of synthetic musks[J]. Acta Scientiae Circumstantiae, 2008, 28(1): 1-11(in Chinese)
曹中利. 石油污染土壤生物强化修复效果及毒性变化研究[D]. 西安: 西安建筑科技大学, 2017: 13-23 Cao Z L. Study on biological enhanced remediation effect and toxicity change of petroleum contaminated soil[D]. Xi’an: Xi’an University of Architecture and Technology, 2017: 13 -23(in Chinese)
李晓斌, 孙寓姣, 王红旗, 等. 焦化厂污染土壤中多环芳烃降解菌群解析[J]. 化工学报, 2010, 61(2): 477-483 Li X B, Sun Y J, Wang H Q, et al. Analysis of PAHs-degrading bacteria from contaminated soil at a coking plant[J]. CIESC Journal, 2010, 61(2): 477-483(in Chinese)
欧志龙, 韩振为, 张瑞玲, 等. Chryseobacterium sp.降解β-环糊精包埋的甲基叔丁基醚[J]. 农业环境科学学报, 2007, 26(4): 1459-1463 Ou Z L, Han Z W, Zhang R L, et al. Chryseobacterium sp. degradation of methyl tert-butyl ether embedded in β-cyclodextrin[J]. Journal of Agro-Environment Science, 2007, 26(4): 1459-1463(in Chinese)
陈燕, 周孙全, 郑奇士, 等. 常温纤维素降解菌的分离与鉴定[J]. 上海交通大学学报(医学版), 2010, 30(8): 1018-1020 Chen Y, Zhou S Q, Zheng Q S, et al. Isolation and identification of cellulose-degrading bacteria under room temperature[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2010, 30(8): 1018-1020(in Chinese) 张逸飞, 顾挺, 王国祥, 等. 一株苯胺降解菌的分离鉴定及其降解特性[J]. 环境污染与防治, 2008, 30(2): 12-15 , 36 Zhang Y F, Gu T, Wang G X, et al. Isolation and characterization of aniline-degrading bacterial strain AN4[J]. Environmental Pollution & Control, 2008, 30(2): 12-15, 36(in Chinese)
解开治, 徐培智, 陈建生, 等. 乙酰甲胺磷降解菌株XP-3的分离鉴定及其生理特性研究[J]. 广东农业科学, 2008(8): 79-82 Xie K Z, Xu P Z, Chen J S, et al. Isolation and identification acephate-degrading bacteria XP-3 and studies on its physiological characterization[J]. Guangdong Agricultural Sciences, 2008(8): 79-82(in Chinese)
姜中玉, 陈育如, 刘虎. 一株将甜菊苷转化为甜茶甙的细菌鉴定及转化特性[J]. 微生物学报, 2011, 51(1): 43-49 Jiang Z Y, Chen Y R, Liu H. Identification and biotransformation properties of a bacterium that converts stevioside into rubusoside[J]. Acta Microbiologica Sinica, 2011, 51(1): 43-49(in Chinese)
曾诚, 范远鑫, 丁少军. 甾醇酯酶菌株Chryseobacterium sp.的筛选及酶学性质研究[J]. 林产化学与工业, 2013, 33(3): 43-49 Zeng C, Fan Y X, Ding S J. Screening of sterol esterase-producing strain Chryseobacterium sp. and its emzymatic properties[J]. Chemistry and Industry of Forest Products, 2013, 33(3): 43-49(in Chinese)
李洋, 胥晓雯, 杨凯晴, 等. 一株高效羽毛降解菌的筛选与鉴定[J]. 生物加工过程, 2022, 20(3): 270-276 Li Y, Xu X W, Yang K Q, et al. Screening of a highly efficient feather degrading bacterium[J]. Chinese Journal of Bioprocess Engineering, 2022, 20(3): 270-276(in Chinese)
王巧蕊, 范宇睿, 郑春莉, 等. 一株金黄杆菌对2-吡啶甲酸的好氧生物降解[J]. 微生物学通报, 2018, 45(5): 990-995 Wang Q R, Fan Y R, Zheng C L, et al. Aerobic biodegradation of 2-picolinic acid by a Chryseobacterium sp.[J]. Microbiology China, 2018, 45(5): 990-995(in Chinese)
赵天涛, 何成明, 张丽杰, 等. 矿化垃圾中金黄杆菌甲烷亲和氧化动力学[J]. 化工学报, 2011, 62(7): 1915-1921 Zhao T T, He C M, Zhang L J, et al. Kinetics of affinity to methane oxidation by Chryseobacterium sp. from aged-refuse[J]. CIESC Journal, 2011, 62(7): 1915-1921(in Chinese)
Zhou B H, Yuan R F, Shi C H, et al. Biodegradation of geosmin in drinking water by novel bacteria isolated from biologically active carbon[J]. Journal of Environmental Sciences (China), 2011, 23(5): 816-823 郭维强, 胡吉军, 陈元芬, 等. 稳定混合菌协同降解咔唑研究[J]. 应用与环境生物学报, 2008, 14(5): 654-657 Guo W Q, Hu J J, Chen Y F, et al. Synergetic degradation of carbazole by a stable microbial consortium[J]. Chinese Journal of Applied and Environmental Biology, 2008, 14(5): 654-657(in Chinese)
尹艳华, 胡学敏, 付民. 利用PCR-DGGE技术鉴定硝基甲苯类物质降解菌株[J]. 北京理工大学学报, 2011, 31(1): 100-104 Yin Y H, Hu X M, Fu M. Determination on high effective strains of degrading nitrotoluene using PCR-DGGE[J]. Transactions of Beijing Institute of Technology, 2011, 31(1): 100-104(in Chinese)
范思艺, 陈芳艳, 唐玉斌, 等. 1株金黄杆菌的分离鉴定及其对DBP的降解特性[J]. 环境科学与技术, 2018, 41(S1): 41-46 Fan S Y, Chen F Y, Tang Y B, et al. Isolation, identification of a Chryseobacterium strain and its degradation characteristics for DBP[J]. Environmental Science & Technology, 2018, 41(S1): 41-46(in Chinese)
陈芳, 周启星. 模拟城市径流中加乐麝香和镉对大型水蚤的毒性效应[J]. 中国环境科学, 2009, 29(1): 58-62 Chen F, Zhou Q X. Toxic effects of galaxolide and cadmium on Daphnia magna under polluting flow conditions containing soil-water interfaces from urban areas[J]. China Environmental Science, 2009, 29(1): 58-62(in Chinese)
Riedel J, Dekant W. Biotransformation and toxicokinetics of musk xylene in humans[J]. Toxicology and Applied Pharmacology, 1999, 157(2): 145-155 Bester K. Retention characteristics and balance assessment for two polycyclic musk fragrances (HHCB and AHTN) in a typical German sewage treatment plant[J]. Chemosphere, 2004, 57(8): 863-870 -
点击查看大图
计量
- 文章访问数: 296
- HTML全文浏览数: 296
- PDF下载数: 62
- 施引文献: 0
下载:
