佳乐麝香降解菌的筛选、鉴定及降解特性研究

王晓晗, 律泽, 张驰, 王宇佳, 吴雨阳, 姚思泉, 孙丽. 佳乐麝香降解菌的筛选、鉴定及降解特性研究[J]. 生态毒理学报, 2024, 19(6): 128-139. doi: 10.7524/AJE.1673-5897.20240730002
引用本文: 王晓晗, 律泽, 张驰, 王宇佳, 吴雨阳, 姚思泉, 孙丽. 佳乐麝香降解菌的筛选、鉴定及降解特性研究[J]. 生态毒理学报, 2024, 19(6): 128-139. doi: 10.7524/AJE.1673-5897.20240730002
WANG Xiaohan, LÜ Ze, ZHANG Chi, WANG Yujia, WU Yuyang, YAO Siquan, SUN Li. Screening, Identification and Degradation Characteristics of Galaxolide Degrading Bacteria[J]. Asian journal of ecotoxicology, 2024, 19(6): 128-139. doi: 10.7524/AJE.1673-5897.20240730002
Citation: WANG Xiaohan, LÜ Ze, ZHANG Chi, WANG Yujia, WU Yuyang, YAO Siquan, SUN Li. Screening, Identification and Degradation Characteristics of Galaxolide Degrading Bacteria[J]. Asian journal of ecotoxicology, 2024, 19(6): 128-139. doi: 10.7524/AJE.1673-5897.20240730002

佳乐麝香降解菌的筛选、鉴定及降解特性研究

    作者简介: 王晓晗(2000—),女,硕士研究生,研究方向为污染土壤水体生态修复,E-mail:2057005103@qq.com
    通讯作者: 律泽(1982-),女,博士,副教授,主要研究方向为污染生态与污染微生物修复、新型污染物环境行为与控制等。E-mail:lvze_2006@163.com; 
  • 基金项目:

    辽宁省教育厅服务地方项目(批准号:JYTMS20231557);辽宁省教育厅青年项目(LJ212410153007);兴安盟本级科技合作项目(WLFZ2024001)

  • 中图分类号: X171.5

Screening, Identification and Degradation Characteristics of Galaxolide Degrading Bacteria

    Corresponding author: LÜ Ze, lvze_2006@163.com
  • Fund Project:
  • 摘要: 佳乐麝香(HHCB)为药品与个人护理品(PPCPs)类新型污染物之一,因其特有的持久性和亲脂性,在环境介质中广泛积累,造成了一定程度的环境污染。为探究人工合成麝香的微生物修复机制,采用富集、分离、驯化的方法,从受污染的土壤样品筛选到一株能利用HHCB生长并具有较好降解能力的菌株H10。根据菌种形态特征、生理生化分析及16S rRNA序列比对结果,初步鉴定其为金黄杆菌属(Chryseobacterium sp.)细菌。该菌降解HHCB的最适宜降解条件为:接种量5%、pH 7.0、温度30 ℃,72 h后对200 mg·L-1的HHCB降解率为68.07%。响应面实验结果显示:当接种量为5.95%,pH为7.4,温度为30.55 ℃、底物浓度为200 mg·L-1时,72 h后菌体密度可达0.662,优化后菌株在此条件下对HHCB的降解率为70.15%。研究发现菌株H10具有降解HHCB的能力,不仅丰富了HHCB降解菌库,也为受人工合成麝香污染环境的微生物修复提供了理论基础。
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  • 收稿日期:  2024-07-30
王晓晗, 律泽, 张驰, 王宇佳, 吴雨阳, 姚思泉, 孙丽. 佳乐麝香降解菌的筛选、鉴定及降解特性研究[J]. 生态毒理学报, 2024, 19(6): 128-139. doi: 10.7524/AJE.1673-5897.20240730002
引用本文: 王晓晗, 律泽, 张驰, 王宇佳, 吴雨阳, 姚思泉, 孙丽. 佳乐麝香降解菌的筛选、鉴定及降解特性研究[J]. 生态毒理学报, 2024, 19(6): 128-139. doi: 10.7524/AJE.1673-5897.20240730002
WANG Xiaohan, LÜ Ze, ZHANG Chi, WANG Yujia, WU Yuyang, YAO Siquan, SUN Li. Screening, Identification and Degradation Characteristics of Galaxolide Degrading Bacteria[J]. Asian journal of ecotoxicology, 2024, 19(6): 128-139. doi: 10.7524/AJE.1673-5897.20240730002
Citation: WANG Xiaohan, LÜ Ze, ZHANG Chi, WANG Yujia, WU Yuyang, YAO Siquan, SUN Li. Screening, Identification and Degradation Characteristics of Galaxolide Degrading Bacteria[J]. Asian journal of ecotoxicology, 2024, 19(6): 128-139. doi: 10.7524/AJE.1673-5897.20240730002

佳乐麝香降解菌的筛选、鉴定及降解特性研究

    通讯作者: 律泽(1982-),女,博士,副教授,主要研究方向为污染生态与污染微生物修复、新型污染物环境行为与控制等。E-mail:lvze_2006@163.com; 
    作者简介: 王晓晗(2000—),女,硕士研究生,研究方向为污染土壤水体生态修复,E-mail:2057005103@qq.com
  • 1. 沈阳建筑大学市政与环境工程学院, 沈阳 110168;
  • 2. 中国建筑第四工程局有限公司, 沈阳 110000
基金项目:

辽宁省教育厅服务地方项目(批准号:JYTMS20231557);辽宁省教育厅青年项目(LJ212410153007);兴安盟本级科技合作项目(WLFZ2024001)

摘要: 佳乐麝香(HHCB)为药品与个人护理品(PPCPs)类新型污染物之一,因其特有的持久性和亲脂性,在环境介质中广泛积累,造成了一定程度的环境污染。为探究人工合成麝香的微生物修复机制,采用富集、分离、驯化的方法,从受污染的土壤样品筛选到一株能利用HHCB生长并具有较好降解能力的菌株H10。根据菌种形态特征、生理生化分析及16S rRNA序列比对结果,初步鉴定其为金黄杆菌属(Chryseobacterium sp.)细菌。该菌降解HHCB的最适宜降解条件为:接种量5%、pH 7.0、温度30 ℃,72 h后对200 mg·L-1的HHCB降解率为68.07%。响应面实验结果显示:当接种量为5.95%,pH为7.4,温度为30.55 ℃、底物浓度为200 mg·L-1时,72 h后菌体密度可达0.662,优化后菌株在此条件下对HHCB的降解率为70.15%。研究发现菌株H10具有降解HHCB的能力,不仅丰富了HHCB降解菌库,也为受人工合成麝香污染环境的微生物修复提供了理论基础。

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