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过量硒-L-蛋氨酸对青鳉鱼胚胎的毒性作用及与氧化应激机制的关联

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周旖妮, 王欢, 杨景峰, 于永利, 董武. 过量硒-L-蛋氨酸对青鳉鱼胚胎的毒性作用及与氧化应激机制的关联[J]. 生态毒理学报, 2022, 17(6): 365-375. doi: 10.7524/AJE.1673-5897.20220207001
引用本文: 周旖妮, 王欢, 杨景峰, 于永利, 董武. 过量硒-L-蛋氨酸对青鳉鱼胚胎的毒性作用及与氧化应激机制的关联[J]. 生态毒理学报, 2022, 17(6): 365-375. doi: 10.7524/AJE.1673-5897.20220207001
shu
Zhou Yini, Wang Huan, Yang Jingfeng, Yu Yongli, Dong Wu. Toxicity of Excess Selenium-L-methionine on Medaka Embryos and Its Relationship with Oxidative Stress Mechanism[J]. Asian journal of ecotoxicology, 2022, 17(6): 365-375. doi: 10.7524/AJE.1673-5897.20220207001
Citation: Zhou Yini, Wang Huan, Yang Jingfeng, Yu Yongli, Dong Wu. Toxicity of Excess Selenium-L-methionine on Medaka Embryos and Its Relationship with Oxidative Stress Mechanism[J]. Asian journal of ecotoxicology, 2022, 17(6): 365-375. doi: 10.7524/AJE.1673-5897.20220207001
shu

过量硒-L-蛋氨酸对青鳉鱼胚胎的毒性作用及与氧化应激机制的关联

  • 1. 内蒙古自治区毒物监控及毒理学重点实验室，内蒙古民族大学动物科技学院，通辽028000;

  • 2. 内蒙古民族大学生命科学与食品学院，通辽 028000

    • 作者简介: 周旖妮(1996-),女,硕士研究生,研究方向为生态毒理学,E-mail:zhouyini96@163.com
    通讯作者: 于永利, E-mail: 707928940@qq.com ;  董武, E-mail: dongwu@imun.edu.cn
  • 基金项目:

    内蒙古自治区自然科学基金资助项目(2021LHMS02007，2020MS08103)；内蒙古自治区高等学校科学研究项目(NJZC17203)；内蒙古自治区高等学校科学研究项目(NJZY22462)；内蒙古民族大学科学研究项目(NMDYB18019)；内蒙古民族大学博士科研启动基金项目(BS555)

  • 中图分类号: X171.5

Toxicity of Excess Selenium-L-methionine on Medaka Embryos and Its Relationship with Oxidative Stress Mechanism

  • 1. Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao 028000, China;

  • 2. College of Life Sciences and Food Engineering, Inner Mongolia Minzu University, Tongliao 028000, China

    • Corresponding authors: Yu Yongli, 707928940@qq.com ;  Dong Wu, dongwu@imun.edu.cn
  • Fund Project:

摘要

  • 摘要: 采矿和农业经营过程中硒(Se)会释放到水生生态系统中造成环境污染。本研究使用日本青鳉鱼(Oryzias latipes)胚胎作为模式动物研究硒代蛋氨酸(SeMet)的发育毒性和潜在的氧化应激机制。给6～7 hpf (hours post-fertilization, hpf)的青鳉鱼胚胎暴露0 (对照)、0.1、10、100、500和1 000 μmol·L-1的SeMet，并检测胚胎存活率、孵化率、形态学和基因表达，并考察氧化应激关联代谢物质的变化。ICP-MS数据显示有机硒通过绒毛膜影响发育中的胚胎。10～1 000 μmol·L-1 SeMet显著降低了青鳉胚胎的存活率(32.1%～0%)，100～1 000 μmol·L-1 SeMet显著减少了孵化率(58%～0%)，例外的是10 μmol·L-1浓度组的死亡率与最高浓度组的相当。10～500 μmol·L-1 SeMet造成血液色素强度显著降低(50%～87.5%，P<0.05)，此外，SeMet暴露造成部分胚胎的心囊水肿、鱼鳔缺失等表型，总发生率超过51%(P<0.05)，也造成游泳行为变化，但差异不显著(P>0.05)。100 μmol·L-1 SeMet也引起氧化应激遗传标记物Nrf2 mRNA(2.1倍，P<0.05)和GPx4 mRNA基因表达的显著增加(3.7倍，P<0.05)。此外发现10～100 μmol·L-1的SeMet引起超氧化物歧化酶(SOD)活性的降低(31%～32.8%，P<0.05)，也同时引起活性氧(ROS)(1.37倍～1.23倍，P<0.05)和丙二醛(MDA)(1.39倍～1.40倍，P<0.05)含量的升高。研究表明，SeMet暴露造成青鳉鱼的胚胎毒性(包括形态学和行为学变化)可能与硒造成的氧化应激相关，也可推知有机硒的过量摄入可能会影响人体健康，尤其是在怀孕期间。
    Abstract: Selenium (Se) can be released into aquatic ecosystem during mining and agricultural operations, resulting in environmental pollution. We used Japanese medaka (Oryzias latipes) embryos as model animals to investigate the developmental toxicity and potential oxidative stress mechanisms of selenomethionine (SeMet). 6～7 hours post-fertilization (hpf) medaka embryos were exposed to 0 (control), 0.1, 10, 100, 500 and 1 000 μmol·L-1 SeMet, and embryo survival, hatchability, morphology, gene expression, and changes in metabolites associated with oxidative stress were examined. ICP-MS data showed that organic selenium affects developing embryos through the chorionic membrane. 10～1 000 μmol·L-1 SeMet significantly reduced the survival rate (32.1% to 0%) and 100～1 000 μmol·L-1 SeMet significantly reduced hatchability (58% to 0%), The exception is that the mortality rate of the 10 μmol·L-1 concentration group showed a non-dose-response relationship compared to the highest concentration group. 10～500 μmol·L-1 SeMet resulted in a significant decrease in blood pigment intensity (50%～87.5%, P<0.05). In addition, SeMet exposure resulted in pericardial edema and loss of swim bladder in some embryos, with a total incidence of more than 51% (P<0.05), and also resulted in swimming behavior change but no significant differences (P>0.05). 100 μmol·L-1 SeMet also significantly increased Nrf2 mRNA (2.1 folds, P<0.05) and GPx4 mRNA gene expression (3.7 fold, P<0.05). In addition, it was found that 10～100 μmol·L-1 SeMet decreased superoxide dismutase (SOD) (31%～32.8%, P<0.05), and increased reactive oxygen species (ROS) (1.37～1.23 times, P<0.05) and malondialdehyde (MDA) (1.39～1.40 times, P<0.05). These results suggest that SeMet exposure may be associated with oxidative stress induced by selenium in medaka. It is supposed that excessive intake of organic selenium may affect human health, especially during pregnancy.
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  • 收稿日期:  2022-02-07
周旖妮, 王欢, 杨景峰, 于永利, 董武. 过量硒-L-蛋氨酸对青鳉鱼胚胎的毒性作用及与氧化应激机制的关联[J]. 生态毒理学报, 2022, 17(6): 365-375. doi: 10.7524/AJE.1673-5897.20220207001
引用本文: 周旖妮, 王欢, 杨景峰, 于永利, 董武. 过量硒-L-蛋氨酸对青鳉鱼胚胎的毒性作用及与氧化应激机制的关联[J]. 生态毒理学报, 2022, 17(6): 365-375. doi: 10.7524/AJE.1673-5897.20220207001
shu
Zhou Yini, Wang Huan, Yang Jingfeng, Yu Yongli, Dong Wu. Toxicity of Excess Selenium-L-methionine on Medaka Embryos and Its Relationship with Oxidative Stress Mechanism[J]. Asian journal of ecotoxicology, 2022, 17(6): 365-375. doi: 10.7524/AJE.1673-5897.20220207001
Citation: Zhou Yini, Wang Huan, Yang Jingfeng, Yu Yongli, Dong Wu. Toxicity of Excess Selenium-L-methionine on Medaka Embryos and Its Relationship with Oxidative Stress Mechanism[J]. Asian journal of ecotoxicology, 2022, 17(6): 365-375. doi: 10.7524/AJE.1673-5897.20220207001
shu

过量硒-L-蛋氨酸对青鳉鱼胚胎的毒性作用及与氧化应激机制的关联

    通讯作者: 于永利, E-mail: 707928940@qq.com ;  董武, E-mail: dongwu@imun.edu.cn
    作者简介: 周旖妮(1996-),女,硕士研究生,研究方向为生态毒理学,E-mail:zhouyini96@163.com
  • 1. 内蒙古自治区毒物监控及毒理学重点实验室，内蒙古民族大学动物科技学院，通辽028000;
  • 2. 内蒙古民族大学生命科学与食品学院，通辽 028000
  • 收稿日期:  2022-02-07
基金项目:

内蒙古自治区自然科学基金资助项目(2021LHMS02007，2020MS08103)；内蒙古自治区高等学校科学研究项目(NJZC17203)；内蒙古自治区高等学校科学研究项目(NJZY22462)；内蒙古民族大学科学研究项目(NMDYB18019)；内蒙古民族大学博士科研启动基金项目(BS555)

摘要: 采矿和农业经营过程中硒(Se)会释放到水生生态系统中造成环境污染。本研究使用日本青鳉鱼(Oryzias latipes)胚胎作为模式动物研究硒代蛋氨酸(SeMet)的发育毒性和潜在的氧化应激机制。给6～7 hpf (hours post-fertilization, hpf)的青鳉鱼胚胎暴露0 (对照)、0.1、10、100、500和1 000 μmol·L-1的SeMet，并检测胚胎存活率、孵化率、形态学和基因表达，并考察氧化应激关联代谢物质的变化。ICP-MS数据显示有机硒通过绒毛膜影响发育中的胚胎。10～1 000 μmol·L-1 SeMet显著降低了青鳉胚胎的存活率(32.1%～0%)，100～1 000 μmol·L-1 SeMet显著减少了孵化率(58%～0%)，例外的是10 μmol·L-1浓度组的死亡率与最高浓度组的相当。10～500 μmol·L-1 SeMet造成血液色素强度显著降低(50%～87.5%，P<0.05)，此外，SeMet暴露造成部分胚胎的心囊水肿、鱼鳔缺失等表型，总发生率超过51%(P<0.05)，也造成游泳行为变化，但差异不显著(P>0.05)。100 μmol·L-1 SeMet也引起氧化应激遗传标记物Nrf2 mRNA(2.1倍，P<0.05)和GPx4 mRNA基因表达的显著增加(3.7倍，P<0.05)。此外发现10～100 μmol·L-1的SeMet引起超氧化物歧化酶(SOD)活性的降低(31%～32.8%，P<0.05)，也同时引起活性氧(ROS)(1.37倍～1.23倍，P<0.05)和丙二醛(MDA)(1.39倍～1.40倍，P<0.05)含量的升高。研究表明，SeMet暴露造成青鳉鱼的胚胎毒性(包括形态学和行为学变化)可能与硒造成的氧化应激相关，也可推知有机硒的过量摄入可能会影响人体健康，尤其是在怀孕期间。

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