3种氟喹诺酮联合暴露对铜锈环棱螺的急性致死效应
Acute Lethal Effects of Combined Exposure to 3 Fluoroquinolones on Bellamya aeruginosa
-
摘要: 氟喹诺酮类抗生素(fluoroquinolones, FQs)的大量使用导致其不可避免地进入水环境中，并对水生生物产生毒性作用。当前关于FQs生态毒理的研究大多是基于单独的某种FQs，而关于不同FQs间联合作用的研究较少。本文以铜锈环棱螺(Bellamya aeruginosa)幼体为受试生物，首先分别研究了环丙沙星(ciprofloxacin, CIP)、诺氟沙星(norfloxacin, NOR)和左氧氟沙星(levofloxacin, LEVO)3种FQs的急性致死效应，随后考察了3种FQs的联合毒性作用。结果表明，单一FQ暴露下随着暴露浓度升高和时间的延长，死亡率均呈现上升趋势，3种FQs对铜锈环棱螺的急性毒性作用依次为NOR(LC50: 59.212 mg·L-1)>CIP(LC50: 114.255 mg·L-1)>LEVO(LC50: 123.706 mg·L-1)。铜锈环棱螺对FQs暴露较为敏感，在FQs生态风险评价中有较大应用潜力。联合暴露结果表明，二元系统及三元系统联合毒性作用有所区别，与FQs种类、浓度和暴露时间长短有关，但最终都趋向于协同作用。本研究结果指示虽然FQs单一暴露毒性较弱，但不同FQs的联合作用导致毒性有所增强，应当予以重视并开展深入研究。Abstract: The massive use of fluoroquinolones (FQs) has led to their inevitable introduction into the aquatic environment and their toxic effects on aquatic organisms. Most of the current research on the ecotoxicology of FQs is based on individual FQs, and there are fewer studies on the combined effects of different FQs. In this study, the acute lethal effects of three FQs, ciprofloxacin (CIP), norfloxacin (NOR), and levofloxacin (LEVO), were investigated separately using Bellamya aeruginosa larvae as test organisms. And the combined toxic effects of the three FQs were investigated. The results showed that the mortality enhanced with the increased of exposure concentration and time. The acute lethal effect of the three FQs on Bellamya aeruginosa were in the order of NOR (LC50: 59.212 mg·L-1)>CIP (LC50: 114.255 mg·L-1)>LEVO (LC50: 123.706 mg·L-1). Bellamya aeruginosa is sensitive to FQs exposure and has great application potential in FQs ecological risk assessment. The combined exposure results showed that the joint toxicity effects of binary and ternary system differed and were related to the type, concentration and duration of exposure to FQs, but eventually tended to be synergistic. The results of this study indicate that although the toxicity of a single FQs is relatively weak, but the combined action of different FQs resulted in enhanced toxicity, which should be paid more attention to and carried out further research.
-
Key words:
- fluoroquinolones /
- Bellamya aeruginosa /
- acute lethal effect /
- combined toxicity
-
-
沙乃庆, 李艳红. 氟喹诺酮类抗生素水污染现状及去除技术研究进展[J]. 工业水处理, 2021, 41(5):22-28 Sha N Q, Li Y H. Current situation of water pollution and research progress treatment technology of fluoroquinolone antibiotics[J]. Industrial Water Treatment, 2021, 41(5):22-28(in Chinese)
Xiao C Q, Han Y, Liu Y, et al. Relationship between fluoroquinolone structure and neurotoxicity revealed by zebrafish neurobehavior[J]. Chemical Research in Toxicology, 2018, 31(4):238-250 Dalla Bona M, Lizzi F, Borgato A, et al. Increasing toxicity of enrofloxacin over four generations of Daphnia magna[J]. Ecotoxicology and Environmental Safety, 2016, 132:397-402 Klein E Y, van Boeckel T P, Martinez E M, et al. Global increase and geographic convergence in antibiotic consumption between 2000 and 2015[J]. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115(15):E3463-E3470 Manzetti S, Ghisi R. The environmental release and fate of antibiotics[J]. Marine Pollution Bulletin, 2014, 79(1-2):7-15 孙秋根, 王智源, 董建玮, 等. 太湖流域河网4种典型抗生素的时空分布和风险评价[J]. 环境科学学报, 2018, 38(11):4400-4410 Sun Q G, Wang Z Y, Dong J W, et al. Spatial-temporal distribution and risk evaluation of four typical antibiotics in river networks of Taihu Lake Basin[J]. Acta Scientiae Circumstantiae, 2018, 38(11):4400-4410(in Chinese)
Valdés M E, Santos L H M L M, Rodríguez Castro M C, et al. Distribution of antibiotics in water, sediments and biofilm in an urban river (Córdoba, Argentina, LA)[J]. Environmental Pollution, 2021, 269:116133 付雨, 剧泽佳, 付耀萱, 等. 白洋淀优势水生植物中喹诺酮类抗生素的生物富集特征及其与环境因子相关性研究[J]. 环境科学学报, 2021, 41(9):3620-3630 Fu Y, Ju Z J, Fu Y X, et al. The bioaccumulation of quinolones (QNs) in the dominant macrophytes and the correlation with environmental factors in Baiyangdian Lake[J]. Acta Scientiae Circumstantiae, 2021, 41(9):3620-3630(in Chinese)
Wagil M, Kumirska J, Stolte S, et al. Development of sensitive and reliable LC-MS/MS methods for the determination of three fluoroquinolones in water and fish tissue samples and preliminary environmental risk assessment of their presence in two rivers in northern Poland[J]. The Science of the Total Environment, 2014, 493:1006-1013 Grenni P, Ancona V, Caracciolo A. Ecological effects of antibiotics on natural ecosystems:A review[J]. Microchemical Journal, 2018, 136:25-39 Yang C, Song G, Lim W. A review of the toxicity in fish exposed to antibiotics[J]. Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology, 2020, 237:108840 Xi J L, Liu J, He S J, et al. Effects of norfloxacin exposure on neurodevelopment of zebrafish (Danio rerio) embryos[J]. Neurotoxicology, 2019, 72:85-94 Liang X M, Wang F, Li K, et al. Effects of norfloxacin nicotinate on the early life stage of zebrafish (Danio rerio):Developmental toxicity, oxidative stress and immunotoxicity[J]. Fish & Shellfish Immunology, 2020, 96:262-269 Wang G X, Zhang Q, Li J L, et al. Combined effects of erythromycin and enrofloxacin on antioxidant enzymes and photosynthesis-related gene transcription in Chlorella vulgaris[J]. Aquatic Toxicology, 2019, 212:138-145 Du J, Zhao H X, Liu S S, et al. Antibiotics in the coastal water of the South Yellow Sea in China:Occurrence, distribution and ecological risks[J]. The Science of the Total Environment, 2017, 595:521-527 Liu K, Yin X F, Zhang D L, et al. Distribution, sources, and ecological risk assessment of quinotone antibiotics in the surface sediments from Jiaozhou Bay wetland, China[J]. Marine Pollution Bulletin, 2018, 129(2):859-865 中华人民共和国生态环境部. 化学物质环境与健康危害评估技术导则(试行)[S]. 北京:中华人民共和国生态环境部, 2020 He S W, Sun Q Y, Liu Y, et al. Acute toxicity, bioaccumulation, and bioreduction of two antibiotics, norfloxacin and ofloxacin, in the snail Bellamya aeruginosa[J]. Water, Air, & Soil Pollution, 2020, 231(3):96 Ma T W, Wang M, Gong S J, et al. Impacts of sediment organic matter content and pH on ecotoxicity of coexposure of TiO2 nanoparticles and cadmium to freshwater snails Bellamya aeruginosa[J]. Archives of Environmental Contamination and Toxicology, 2017, 72(1):153-165 Li Q, Wang M, Duan L, et al. Multiple biomarker responses in caged benthic gastropods Bellamya aeruginosa after in situ exposure to Taihu Lake in China[J]. Environmental Sciences Europe, 2018, 30(1):34 Ma T W. Laboratory culture of the freshwater benthic gastropod Bellamya aeruginosa (Reeve) and its utility as a test species for sediment toxicity[J]. Journal of Environmental Sciences, 2010, 22(2):304-313 徐怡璐, 吴彬, 石倩倩, 等. 锰铅镉复合暴露对人神经母细胞瘤细胞的毒性作用[J]. 环境与健康杂志, 2019, 36(8):679-684 Xu Y L, Wu B, Shi Q Q, et al. Toxicity of co-exposure to manganese, lead and cadmium to SK-N-SH cells[J]. Journal of Environment and Health, 2019, 36(8):679-684(in Chinese)
汪皓琦, 董玉瑛, 汪灵伟. 3种新型污染物对发光菌的毒性作用研究[J]. 生态毒理学报, 2018, 13(4):179-184 Wang H Q, Dong Y Y, Wang L W. Study on the toxicity of three emerging pollutants to Photobacterium phosphoreum[J]. Asian Journal of Ecotoxicology, 2018, 13(4):179-184(in Chinese)
Peltzer P M, Lajmanovich R C, Attademo A M, et al. Ecotoxicity of veterinary enrofloxacin and ciprofloxacin antibiotics on anuran amphibian larvae[J]. Environmental Toxicology and Pharmacology, 2017, 51:114-123 Shen R, Yu Y C, Lan R, et al. The cardiovascular toxicity induced by high doses of gatifloxacin and ciprofloxacin in zebrafish[J]. Environmental Pollution, 2019, 254(Pt B):112861 中华人民共和国国家环境保护总局. 新化学物质危害评估导则:HJ/T 154-2004[S]. 北京:中华人民共和国国家环境保护总局, 2004 Riaz L, Mahmood T, Coyne M S, et al. Physiological and antioxidant response of wheat (Triticum aestivum) seedlings to fluoroquinolone antibiotics[J]. Chemosphere, 2017, 177:250-257 Magdaleno A, Saenz M E, Juarez A B, et al. Effects of six antibiotics and their binary mixtures on growth of Pseudokirchneriella subcapitata[J]. Ecotoxicology and Environmental Safety, 2015, 113:72-78 Jia J, Zhu F, Ma X H, et al. Mechanisms of drug combinations:Interaction and network perspectives[J]. Nature Reviews Drug Discovery, 2009, 8(2):111-128 Xu X Q, Lu Q, Yang Y, et al. A proposed "steric-like effect" for the slowdown of enrofloxacin antibiotic metabolism by ciprofloxacin, and its mechanism[J]. Chemosphere, 2021, 284:131347 Han Y, Ma Y Y, Yao S C, et al. In vivo and in silico evaluations of survival and cardiac developmental toxicity of quinolone antibiotics in zebrafish embryos (Danio rerio)[J]. Environmental Pollution, 2021, 277:116779 Kergaravat S V, Gagneten A M, Hernandez S R. Development of an electrochemical method for the detection of quinolones:Application to cladoceran ecotoxicity studies[J]. Microchemical Journal, 2018, 141:279-286 van Doorslaer X, Dewulf J, van Langenhove H, et al. Fluoroquinolone antibiotics:An emerging class of environmental micropollutants[J]. The Science of the Total Environment, 2014, 500-501:250-269 -
点击查看大图
计量
- 文章访问数: 1618
- HTML全文浏览数: 1618
- PDF下载数: 51
- 施引文献: 0
下载:
