双酚A对合果芋叶片气体交换参数、叶绿素荧光参数和氧化损伤的影响
Effects of Bisphenol A on Gas Exchange Parameters, Chlorophyll Fluorescence Parameters and Oxidative Damage in Leaves of Syngonium podophyllum Schott
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摘要: 目前双酚A(bisphenol A, BPA)已经成为一种全球性的环境污染物。以合果芋(Syngonium podophyllum Schott, SPS)为研究对象，采用水培试验的方式，在0、10、30和50 mg·L-1的BPA处理下，研究了BPA对合果芋叶片光合色素、气体交换参数、叶绿素荧光参数和氧化损伤的影响。研究结果显示，所有BPA处理的合果芋叶片叶绿素a、叶绿素b、总叶绿素和类胡萝卜素的含量均低于对照组(6.4%～32.5%)，且随BPA胁迫浓度和时间的增加呈下降趋势。30 mg·L-1和50 mg·L-1的BPA处理明显降低了合果芋叶片净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)、最大荧光产量(Fm)和PSⅡ最大光能转化效率(Fv/Fm)值，但增加了胞间CO2浓度(Ci)和初始荧光(F0)值。合果芋叶片过氧化氢(H2O2)、丙二醛(MDA)含量和电解质渗透率(EC)在30 mg·L-1和50 mg·L-1的BPA处理下，随着胁迫时间的增加呈现不同程度的递增，而在10 mg·L-1的BPA处理下，3 d内略有上升，而后趋于稳定。上述结果表明在30 mg·L-1和50 mg·L-1的BPA处理下，合果芋的植物生理代谢出现了一定的紊乱现象，而10 mg·L-1的BPA对合果芋的正常生长影响较小。Abstract: Bisphenol A (BPA) has become a global environmental pollutant. The effects of BPA on photosynthetic pigment, gas exchange parameters, chlorophyll fluorescence parameters and oxidative damage in leaves of Syngonium podophyllum Schott (SPS) were investigated in this study. SPS were exposed to BPA with four levels (0, 10, 30, and 50 mg·L-1) through a water culture experiment. The contents of chlorophyll a, chlorophyll b, total chlorophyll and carotenoids in leaves from all BPA treatments were lower than those from the control group (CK, 6.4%～32.5%), and decreased with the increase of BPA stress concentration and time. The treatments of higher concentrations BPA (30 mg·L-1 and 50 mg·L-1) reduced significantly the net photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs), as well as maximum fluorescence yield (Fm) and maximal photochemical efficiency (Fv/Fm), but elevated the intercellular CO2 concentration (Ci) and initial fluorescence (F0). BPA at higher concentrations (30 mg·L-1 and 50 mg·L-1) increased hydrogen peroxide (H2O2) contents, malondialdehyde (MDA) contents, and electrolyte leakage, while lower concentrations (10 mg·L-1) increased in the first 3 d and then trended to stabilize. The present study indicated that BPA could disorder the plant physiological metabolism of SPS at higher concentrations, but had little effect on the normal growth of SPS at lower concentrations.
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