水华鱼腥藻(Anabaena aquae)不同生长阶段的产嗅特征和藻源有机物特性
Characteristics of Taste and Odor Compounds and Algal-derived Organic Matter in Different Growth Stages of Anabaena aquae
-
摘要: 水华鱼腥藻(Anabaena aquae)是一种水体中常见的优势蓝藻，为水中嗅味的重要来源，且生长过程中释放的藻源有机物(AOM)对于水质有较大的影响。研究水华鱼腥藻不同生长阶段的产嗅特征和AOM特性对于藻污染控制有重要意义。建立水华鱼腥藻生长曲线，提取不同生长阶段的胞内与胞外有机物，通过气质联用分析、三维荧光扫描和液相-有机碳测试(liquid chromatography coupled with organic carbon detection, LC-OCD)等表征手段，对水华鱼腥藻生长过程中的胞内外嗅味物质和有机物的变化情况进行多维度解析。结果表明，本实验培养条件下水华鱼腥藻所产的嗅味物质主要是β-紫罗兰酮和β-环柠檬醛，主要存在于胞内有机物，平均浓度为2.90 μg·L-1和1.53 μg·L-1。其产嗅能力随着藻细胞的生长而减弱。AOM呈现出低分子亲水性的特征。三维荧光分析结果表明，胞外有机物主要含有类腐殖质物质和溶解性微生物代谢产物，胞内有机物内主要含有溶解性微生物代谢物质、络氨酸和色氨酸。生长过程中胞内有机物和嗅味物质随藻细胞的破裂逐渐泄露到胞外，胞内外有机物的组分不会发生较大变化，其浓度随生长阶段的不同会发生相应的改变。LC-OCD结果显示，AOM主要由低分子有机物构成，且溶解性有机氮含量较高。Abstract: Anabaena aquae is a common dominant cyanobacterium in water, which is an important source of taste and odor (T&O) compounds in water, and the algal-derived organic matter (AOM) released during its growth phases has a great influence on water quality. It is of great significance to study the characteristics of T&O compounds and AOM in different growth phases of Anabaena aquae for the control of algal pollution. The growth curves of Anabaena aquae were established, and the intracellular and extracellular organic matter at different growth phases were extracted. The changes of intracellular and extracellular organic matter and T&O compounds during the growth of Anabaena aquae were analyzed by gas-mass spectrometry, excitation-emission-matrix spectra (EEMs) and liquid chromatography coupled with organic carbon detection (LC-OCD). It was found that the main T&O compounds produced by Anabaena aquae under this experimental culture condition were β-ionone and β-cyclocitral which mainly existed in the intracellular organic matter, and the average concentrations were 2.90 μg·L-1 and 1.53 μg·L-1. Its ability to produce T&O compounds decreases with the growth of algae. AOM was characterized as low molecular hydrophilic. The results of EEMs showed that extracellular organic matter mainly contained humic-like substances and soluble microbial products, and intracellular organic matter mainly contained soluble microbial products, tyrosine and tryptophan. Intracellular organic matter and T&O compounds gradually leaked to the extracellular with the rupture of algal cells during the growth phases, and no major changes in the components of intracellular and extracellular organic matter were observed, and their concentrations would change accordingly with the growth stages. The results of LC-OCD showed that AOM was mainly composed of low molecular organic matter, and the content of dissolved organic nitrogen was high.
-
-
中华人民共和国生态环境部. 2020年中国生态环境状况公报[R]. 北京: 中华人民共和国生态环境部, 2021 王娜, 葛飞, 吴秀珍, 等. 藻类及其胞外分泌物对净水工艺的影响[J]. 水处理技术, 2010, 36(2): 19-24 Wang N, Ge F, Wu X Z, et al. Effect of algae and its extracellular organic matter on drinking water treatment [J]. Technology of Water Treatment, 2010, 36(2): 19-24 (in Chinese)
曾宁. 紫外光-双氧水高级氧化技术对饮用水中典型致嗅物质去除的研究[D]. 西安: 西安建筑科技大学, 2017: 1-2 Zeng N. Study on removing typical odorants in drinking water by advanced oxidation technology of ultraviolet combined with hydrogen peroxide [D]. Xi’an: Xi’an University of Architecture and Technology, 2017: 1 -2 (in Chinese)
Goslan E H, Seigle C, Purcell D, et al. Carbonaceous and nitrogenous disinfection by-product formation from algal organic matter [J]. Chemosphere, 2017, 170: 1-9 陈伟涛. 大沙河水库富营养化及蓝藻水华特点分析[J]. 广东水利水电, 2015(6): 33-35 Chen W T. Analysis on eutrophication and cyanobacteria blooms characteristics in Dashahe Reservoir [J]. Guangdong Water Resources and Hydropower, 2015 (6): 33-35 (in Chinese)
高胜玲. 太湖水华蓝藻群落和胶鞘多糖代谢及其基因表达模式的研究[D]. 南京: 南京农业大学, 2018: 14-15 Gao S L. Studies on cyanobacterial community and sheath polysaccharides metabolism and their gene expression patterns in Lake Taihu [D]. Nanjing: Nanjing Agricultural University, 2018: 14 -15 (in Chinese)
戴国飞, 刘慧丽, 张伟, 等. 江西柘林湖富营养化现状与藻类时空分布特征[J]. 湖泊科学, 2015, 27(2): 275-281 Dai G F, Liu H L, Zhang W, et al. Spatio-temporal variation of algae and eutrophic status of Lake Zhelin, Jiangxi Province [J]. Journal of Lake Sciences, 2015, 27(2): 275-281 (in Chinese)
贾晓会, 施定基, 史绵红, 等. 巢湖蓝藻水华形成原因探索及“优势种光合假说”[J]. 生态学报, 2011, 31(11): 2968-2977 Jia X H, Shi D J, Shi M H, et al. Formation of cyanobacterial blooms in Lake Chaohu and the photosynthesis of dominant species hypothesis [J]. Acta Ecologica Sinica, 2011, 31(11): 2968-2977 (in Chinese)
邱丽佳. H2O2及O3氧化两种典型蓝藻致嗅物质和灭藻效应研究[D]. 北京: 北京建筑大学, 2017: 40-41 Qiu L J. Oxidation of odor compound and inactivation efficiency of two kinds of typical cyanobacteria by hydrogen peroxide and ozone [D]. Beijing: Beijing University of Civil Engineering and Architecture, 2017: 40 -41 (in Chinese)
陈克云. 水体藻源嗅味物质产生特征与生物降解研究[D]. 济南: 山东师范大学, 2011: 21-29 Chen K Y. Study on odorous compounds production characteristics and biodegradation [D]. Ji’nan: Shandong Normal University, 2011: 21 -29 (in Chinese)
Guo Q Y, Yu J W, Zhao Y Y, et al. Identification of fishy odor causing compounds produced by Ochromonas sp. and Cryptomonas ovate with gas chromatography-olfactometry and comprehensive two-dimensional gas chromatography [J]. Science of the Total Environment, 2019, 671: 149-156 张建芳, 顾青清, 符策竿, 等. 某浅水湖型水源地土臭素(GSM)和2-甲基异莰醇(2-MIB)的变化规律[J]. 净水技术, 2018, 37(11): 7-10 Zhang J F, Gu Q Q, Fu C G, et al. Variation regulation of geosmin (GSM) and 2-methylisoborneol (2-MIB) in a water source of shallow-lake type [J]. Water Purification Technology, 2018, 37(11): 7-10 (in Chinese)
Abrha Y, Kye H, Kwon M, et al. Removal of algae, and taste and odor compounds by a combination of plant-mineral composite (PMC) coagulant with UV-AOPs: Laboratory and pilot scale studies [J]. Applied Sciences, 2018, 8(9): 1502 邱丽佳, 张君枝, 张艳娜, 等. H2O2氧化铜绿微囊藻致嗅物质及灭藻效应研究[J]. 环境科学学报, 2017, 37(3): 954-961 Qiu L J, Zhang J Z, Zhang Y N, et al. Oxidation of odor compound and inactivation efficiency of Microcystis aeruginosa by hydrogen peroxide [J]. Acta Scientiae Circumstantiae, 2017, 37(3): 954-961 (in Chinese)
陈晨. 高藻原水藻类次生嗅味污染特征及光氧化控制技术研究[D]. 杭州: 浙江工业大学, 2012: 32-56 Chen C. Theoretical study on algae secondary odor metabolic pollution characteristics and control by photo-oxidation of high algae raw water [D]. Hangzhou: Zhejiang University of Technology, 2012: 32 -56 (in Chinese)
Guo T T, Yang Y L, Liu R P, et al. Enhanced removal of intracellular organic matters (IOM) from Microcystic aeruginosa by aluminum coagulation [J]. Separation and Purification Technology, 2017, 189: 279-287 Liu R P, Guo T T, Ma M, et al. Preferential binding between intracellular organic matters and Al13 polymer to enhance coagulation performance [J]. Journal of Environmental Sciences, 2019, 76: 1-11 冯桂学, 刘莉, 顿咪娜, 等. 固相萃取-气相色谱-质谱法测定水中10种嗅味物质的含量[J]. 理化检验-化学分册, 2017, 53(5): 502-506 Feng G X, Liu L, Dun M N, et al. SPE-GC-MS determination of 10 odorous materials in water [J]. Physical Testing and Chemical Analysis (Part B: Chemical Analysis), 2017, 53(5): 502-506 (in Chinese)
冯桂学, 宋艳, 刘莉, 等. 固相萃取-气相色谱质谱联用法测定地表水中藻致嗅味物质[J]. 中国环境监测, 2014, 30(6): 163-166 Feng G X, Song Y, Liu L, et al. Determination of taste and odor compounds in surface water using solid phase extraction and gas chromatography-mass spectrometry [J]. Environmental Monitoring in China, 2014, 30(6): 163-166 (in Chinese)
周昀. 生活饮用水卫生标准中有机物的三维荧光特征及应急处理[D]. 杭州: 浙江工业大学, 2016: 3-5 Zhou Y. Fluorescent features and emergency measure of the organic matters in standards for drinking water quality [D]. Hangzhou: Zhejiang University of Technology, 2016: 3 -5 (in Chinese)
Huber S A, Balz A, Abert M, et al. Characterisation of aquatic humic and non-humic matter with size-exclusion chromatography-organic carbon detection-organic nitrogen detection (LC-OCD-OND) [J]. Water Research, 2011, 45(2): 879-885 Li X, Rao N R H, Linge K L, et al. An evaluation of measurement techniques for algal-derived organic nitrogen [J]. Water Research, 2019, 165: 114998 Villacorte L O, Ekowati Y, Neu T R, et al. Characterisation of algal organic matter produced by bloom-forming marine and freshwater algae [J]. Water Research, 2015, 73: 216-230 Suurnäkki S, Gomez-Saez G V, Rantala-Ylinen A, et al. Identification of geosmin and 2-methylisoborneol in cyanobacteria and molecular detection methods for the producers of these compounds [J]. Water Research, 2015, 68: 56-66 李维唯, 郭康宁, 刘莉文, 等. 若干水华相关藻类对太湖水体异味物质贡献的初步研究[J]. 湖泊科学, 2018, 30(4): 916-924 Li W W, Guo K N, Liu L W, et al. Relationship of odor compounds to some algal strains associated with bloom in Lake Taihu [J]. Journal of Lake Sciences, 2018, 30(4): 916-924 (in Chinese)
Young C C, Suffet I H, Crozes G, et al. Identification of a woody-hay odor-causing compound in a drinking water supply [J]. Water Science and Technology, 1999, 40(6): 273-278 李雨轩. 超滤-纳滤双膜组合工艺在高品质饮用水处理中的研究[D]. 兰州: 兰州交通大学, 2019: 61-65 Li Y X. Study on ultrafiltration-nanofiltration combined membrane process in high quality drinking water treatment [D]. Lanzhou: Lanzhou Jiatong University, 2019: 61 -65 (in Chinese)
魏彬. 地表水表观污染特性及其污染物分布规律研究[D]. 苏州: 苏州科技大学, 2019: 44-48 Wei B. Study on the characteristics of surface water pollution and the distribution of pollutants [D]. Suzhou: Suzhou University of Science and Technology, 2019: 44 -48 (in Chinese)
白敏冬, 李海燕, 满化林, 等. 基于常规饮用水工艺羟基自由基处理高藻水[J]. 环境工程学报, 2017, 11(9): 4897-4902 Bai M D, Li H Y, Man H L, et al. Treatment of algae bloom water using ·OH based on conventional drinking water process [J]. Chinese Journal of Environmental Engineering, 2017, 11(9): 4897-4902 (in Chinese)
-
点击查看大图
计量
- 文章访问数: 4725
- HTML全文浏览数: 4725
- PDF下载数: 113
- 施引文献: 0
下载:
