脱氧雪腐镰刀菌烯醇对黄粉虫毒性及其排泄行为

秦菲; 汪洋; 赵祎; 陈明生; 范文佳; 郭宝元; 王松雪

doi:10.7524/AJE.1673-5897.20210616001

生态毒理学报

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秦菲, 汪洋, 赵祎, 陈明生, 范文佳, 郭宝元, 王松雪. 脱氧雪腐镰刀菌烯醇对黄粉虫毒性及其排泄行为[J]. 生态毒理学报, 2022, 17(2): 311-316. doi: 10.7524/AJE.1673-5897.20210616001

引用本文:

Qin Fei, Wang Yang, Zhao Yi, Chen Mingsheng, Fan Wenjia, Guo Baoyuan, Wang Songxue. Toxicity and Excretion of Deoxynivalenol to Tenebrio molitor Larvae[J]. Asian journal of ecotoxicology, 2022, 17(2): 311-316. doi: 10.7524/AJE.1673-5897.20210616001

Citation:

Qin Fei, Wang Yang, Zhao Yi, Chen Mingsheng, Fan Wenjia, Guo Baoyuan, Wang Songxue. Toxicity and Excretion of Deoxynivalenol to Tenebrio molitor Larvae[J]. Asian journal of ecotoxicology, 2022, 17(2): 311-316. doi: 10.7524/AJE.1673-5897.20210616001

脱氧雪腐镰刀菌烯醇对黄粉虫毒性及其排泄行为

1. 北京联合大学生物化学工程学院，北京 100023;
2. 国家粮食和物资储备局科学研究院，北京 100037;
3. 湖北以勒科技有限公司，武汉 430070

作者简介: 秦菲(1977—)，女，博士，副教授，研究方向为食品检测与营养评价，E- mail: qinfei@buu.edu.cn

通讯作者: 郭宝元, E-mail: gby@ags.ac.cn ;

基金项目:
国家重点研发计划(2017YFC1600900)；国家自然科学基金资助项目(31972605)
中图分类号: X171.5

Toxicity and Excretion of Deoxynivalenol to Tenebrio molitor Larvae

1. College of Biochemical Engineering, Beijing Union University, Beijing 100023, China;
2. Academy of National Food and Strategic Reserves Administration, Beijing 100037, China;
3. Hubei Jireh Technology Company Limited, Wuhan 430073, China

Corresponding author: Guo Baoyuan, gby@ags.ac.cn ;

Fund Project:

摘要: 脱氧雪腐镰刀菌烯醇(DON)是影响粮食和饲料安全最重要的真菌毒素之一。为研究DON对黄粉虫的毒性及其排泄过程和行为，采用不同浓度DON(低剂量组0.25 mg·kg^-1，中剂量组5.51 mg·kg^-1，高剂量组8.83 mg·kg^-1)添加的小麦粉对黄粉虫进行暴露。结果表明，低剂量组幼虫存活率(85%)＞高剂量组幼虫存活率(83%)＞中剂量组幼虫存活率(78%)，不同剂量组之间存活率无显著差异(P>0.05)。不同DON剂量组喂养的黄粉虫体质量增加也无显著性差异(P>0.05)。进食低剂量DON黄粉虫粪便中未检出DON。进食中剂量DON的黄粉虫DON排泄率为9%；进食高剂量DON黄粉虫的DON排泄率为17%。
- 呕吐毒素 /
- 黄粉虫 /
- 粪便 /
- 降解 /
- 排泄
Abstract: Deoxynivalenol (DON), produced by various fusarium species, is one of the most prevalent food- and feed-associated mycotoxins. This study concentrated on the toxicity and excretion of DON by dietary exposure for Tenebrio molitor larvae. Three DON levels of 0.25 (low dosage, LD), 5.51 (medium dosage, MD) and 8.83 mg·kg^-1 (high dosage, HD) in wheat flour were employed. The survival rates were as follows: LD (85%)>HD (83%)>MD (78%). There was no significant difference in survival rate (P>0.05) among different groups and body mass increase of larvae (P>0.05). After 24 h, DON was undetectable in the feces of Tenebrio molitor larvae of the low dosage group, while the excreted rates of the medium and high dosage groups were 9% and 17%, respectively.
- deoxynivalenol /
- Tenebrio molitor larvae /
- feces /
- degradation /
- excretion

张静, 张琼琼, 计成, 等. 微生物及生物酶对脱氧雪腐镰刀菌烯醇生物转化研究进展[J]. 动物营养学报, 2020, 32(10): 4807-4820

Zhang J, Zhang Q Q, Ji C, et al. Research advance on biotransformation of deoxynivalenol by microbes and biological enzymes [J]. Chinese Journal of Animal Nutrition, 2020, 32(10): 4807-4820 (in Chinese)

唐语谦, 潘药银, 刘晨迪, 等. 脱氧雪腐镰刀菌烯醇的生物转化及其隐蔽型毒素的形成研究进展[J]. 食品科学, 2020, 41(19): 281-289

Tang Y Q, Pan Y Y, Liu C D, et al. Advances in understanding the biotransformation of deoxynivalenol and its masked forms [J]. Food Science, 2020, 41(19): 281-289 (in Chinese)

Bosch G, Fels-Klerx H J V, Rijk T C, et al. Aflatoxin B1 tolerance and accumulation in black soldier fly larvae (Hermetia illucens) and yellow mealworms (Tenebrio molitor) [J]. Toxins, 2017, 9(6): E185

Niermans K, Woyzichovski J, Kröncke N, et al. Feeding study for the mycotoxin zearalenone in yellow mealworm (Tenebrio molitor) larvae-investigation of biological impact and metabolic conversion [J]. Mycotoxin Research, 2019, 35(3): 231-242

Piacenza N, Kaltner F, Maul R, et al. Distribution of T-2 toxin and HT-2 toxin during experimental feeding of yellow mealworm (Tenebrio molitor) [J]. Mycotoxin Research, 2021, 37(1): 11-21

Camenzuli L, van Dam R, de Rijk T, et al. Tolerance and excretion of the mycotoxins aflatoxin B1, zearalenone, deoxynivalenol, and ochratoxin A by Alphitobius diaperinus and Hermetia illucens from contaminated substrates [J]. Toxins, 2018, 10(2): 91

van Broekhoven S, Gutierrez J M, de Rijk T C, et al. Degradation and excretion of the fusarium toxin deoxynivalenol by an edible insect, the yellow mealworm (Tenebrio molitor L.) [J]. World Mycotoxin Journal, 2017, 10(2): 163-169

Ochoa Sanabria C, Hogan N, Madder K, et al. Yellow mealworm larvae (Tenebrio molitor) fed mycotoxin-contaminated wheat: A possible safe, sustainable protein source for animal feed? [J]. Toxins, 2019, 11(5): E282

Hong J S, Han T, Kim Y Y. Mealworm (Tenebrio molitor larvae) as an alternative protein source for monogastric animal: A review [J]. Animals, 2020, 10(11): 2068

Grau T, Vilcinskas A, Joop G. Degradation and excretion of the Fusarium toxin deoxynivalenol by an edible insect, the Yellow mealworm (Tenebrio molitor L.) [J]. Journal of Biosciences, 2017, 72(9-10): 337-349

Guo B Y, Yin J, Hao W Y, et al. Polyurethane foam induces epigenetic modification of mitochondrial DNA during different metamorphic stages of Tenebrio molitor [J]. Ecotoxicology and Environmental Safety, 2019, 183: 109461

Yang Y, Yang J, Wu W M, et al. Biodegradation and mineralization of polystyrene by plastic-eating mealworms: Part 2. Role of gut microorganisms [J]. Environmental Science & Technology, 2015, 49(20): 12087-12093

中华人民共和国国家卫生和计划生育委员会，国家食品药品监督管理总局, 中华人民共和国国家食品药品监督管理总局. 食品安全国家标准食品中真菌毒素限量: GB 2761—2017[S]. 北京: 中国标准出版社, 2017

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Citation:

脱氧雪腐镰刀菌烯醇对黄粉虫毒性及其排泄行为

通讯作者: 郭宝元, E-mail: gby@ags.ac.cn ;

作者简介: 秦菲(1977—)，女，博士，副教授，研究方向为食品检测与营养评价，E- mail: qinfei@buu.edu.cn
1. 北京联合大学生物化学工程学院，北京 100023;

2. 国家粮食和物资储备局科学研究院，北京 100037;

3. 湖北以勒科技有限公司，武汉 430070

收稿日期: 2021-06-16

基金项目:

国家重点研发计划(2017YFC1600900)；国家自然科学基金资助项目(31972605)

关键词:

呕吐毒素 /
黄粉虫 /
粪便 /
降解 /
排泄

摘要: 脱氧雪腐镰刀菌烯醇(DON)是影响粮食和饲料安全最重要的真菌毒素之一。为研究DON对黄粉虫的毒性及其排泄过程和行为，采用不同浓度DON(低剂量组0.25 mg·kg^-1，中剂量组5.51 mg·kg^-1，高剂量组8.83 mg·kg^-1)添加的小麦粉对黄粉虫进行暴露。结果表明，低剂量组幼虫存活率(85%)＞高剂量组幼虫存活率(83%)＞中剂量组幼虫存活率(78%)，不同剂量组之间存活率无显著差异(P>0.05)。不同DON剂量组喂养的黄粉虫体质量增加也无显著性差异(P>0.05)。进食低剂量DON黄粉虫粪便中未检出DON。进食中剂量DON的黄粉虫DON排泄率为9%；进食高剂量DON黄粉虫的DON排泄率为17%。

English Abstract

Toxicity and Excretion of Deoxynivalenol to Tenebrio molitor Larvae

Corresponding author: Guo Baoyuan, gby@ags.ac.cn ;

1. College of Biochemical Engineering, Beijing Union University, Beijing 100023, China;
2. Academy of National Food and Strategic Reserves Administration, Beijing 100037, China;
3. Hubei Jireh Technology Company Limited, Wuhan 430073, China

Received Date: 2021-06-16

Fund Project:

Keywords:

Abstract: Deoxynivalenol (DON), produced by various fusarium species, is one of the most prevalent food- and feed-associated mycotoxins. This study concentrated on the toxicity and excretion of DON by dietary exposure for Tenebrio molitor larvae. Three DON levels of 0.25 (low dosage, LD), 5.51 (medium dosage, MD) and 8.83 mg·kg^-1 (high dosage, HD) in wheat flour were employed. The survival rates were as follows: LD (85%)>HD (83%)>MD (78%). There was no significant difference in survival rate (P>0.05) among different groups and body mass increase of larvae (P>0.05). After 24 h, DON was undetectable in the feces of Tenebrio molitor larvae of the low dosage group, while the excreted rates of the medium and high dosage groups were 9% and 17%, respectively.