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Guo Xiaoyan, Zhang Zhihong, Shen Qiying, Li Cuiqing. Screening of lube oil biodegradation strain and degrading characteristics[J]. Chinese Journal of Environmental Engineering, 2013, 7(4): 1589-1593.
Citation: Guo Xiaoyan, Zhang Zhihong, Shen Qiying, Li Cuiqing. Screening of lube oil biodegradation strain and degrading characteristics[J]. Chinese Journal of Environmental Engineering, 2013, 7(4): 1589-1593.
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Screening of lube oil biodegradation strain and degrading characteristics

  • 1.

    Department of Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China

  • Received Date: 05/04/2012
    Accepted Date: 20/02/2012
    Available Online: 09/04/2013
    Fund Project:
  • With oil contaminated soil as starting bacterial source and lube oil as sole carbon source, 4 lube oil biodegradation strains were screened. 4 strains were identified as Xanthomonas, Azotobacter, Pseudomonas and Flavobacterium separately, according to their morphological, physiological, and biochemical features. G4 in 4 screened strains had the highest biodegradation rate. Degrading characteristics of strain G4 were studied and among the experimental factors, the culture temperature had the greatest effect on biodegradation rate, followed by the concentration of glucose, the concentration of ammonium sulphate, and pH. Strain G4 could degrade lube oil in the temperature range of 20~40℃. The degradation rate was over 80% in the pH range of 5.0~9.0 at appropriate temperature. When 200 μL lube oil, the sole carbon source, was degraded by 100 mL strain culture medium for 36 h, the biodegradation rate rose to 84.6% under the optimum conditions as follows: ammonium sulphate concentration of 1.0 g/L, pH of 9.0 and 30℃.
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Screening of lube oil biodegradation strain and degrading characteristics

  • Received Date: 05/04/2012
  • Accepted Date: 20/02/2012
  • Available Online: 09/04/2013
Fund Project:

Abstract: With oil contaminated soil as starting bacterial source and lube oil as sole carbon source, 4 lube oil biodegradation strains were screened. 4 strains were identified as Xanthomonas, Azotobacter, Pseudomonas and Flavobacterium separately, according to their morphological, physiological, and biochemical features. G4 in 4 screened strains had the highest biodegradation rate. Degrading characteristics of strain G4 were studied and among the experimental factors, the culture temperature had the greatest effect on biodegradation rate, followed by the concentration of glucose, the concentration of ammonium sulphate, and pH. Strain G4 could degrade lube oil in the temperature range of 20~40℃. The degradation rate was over 80% in the pH range of 5.0~9.0 at appropriate temperature. When 200 μL lube oil, the sole carbon source, was degraded by 100 mL strain culture medium for 36 h, the biodegradation rate rose to 84.6% under the optimum conditions as follows: ammonium sulphate concentration of 1.0 g/L, pH of 9.0 and 30℃.

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