Isolation of an Acidithiobacillus ferrooxidans strain and its performance in bioleaching of copper from waste printed circuit boards

Zhang Ting; Zhu Nengwu; Xu Zhiguo; Shi Chaohong

2013 Volume 7 Issue 4

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Abstract

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Zhang Ting, Zhu Nengwu, Xu Zhiguo, Shi Chaohong. Isolation of an Acidithiobacillus ferrooxidans strain and its performance in bioleaching of copper from waste printed circuit boards[J]. Chinese Journal of Environmental Engineering, 2013, 7(4): 1482-1488.

Citation:

Zhang Ting, Zhu Nengwu, Xu Zhiguo, Shi Chaohong. Isolation of an Acidithiobacillus ferrooxidans strain and its performance in bioleaching of copper from waste printed circuit boards[J]. Chinese Journal of Environmental Engineering, 2013, 7(4): 1482-1488.

Isolation of an Acidithiobacillus ferrooxidans strain and its performance in bioleaching of copper from waste printed circuit boards

1.
College of Environment and Energy, South China University of Technology, Guangzhou 510006, China
2.
Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510006, China

Received Date: 02/04/2012
Accepted Date: 22/02/2012
Available Online: 09/04/2013

Fund Project:

Abstract

An acidophilic bacterium named as Z1 was isolated from the acid mine drainage of a pyrite mine in Guangdong. It was identified as Acidithiobacillus ferrooxidans (A. ferrooxidans) genus by morphology, physiology and near full-length 16S rRNA gene analysis. The strain Z1 and the strains such as A. ferrooxidans strain CMS in GenBank were in the same branch of the phylogenetic tree. The 16S rRNA gene sequence of the strain Z1 had 99% sequence similarity to A. ferrooxidans strain CMS. The optimum growth pH value and temperature, and the logarithmic phase of strain Z1 were 2.25 and 30℃, and from 18 h to 30 h, respectively. The average ferrous oxidation rate of the Z1 reached 0.2307 g/(L·h). The adapted strain Z1 could tolerate 15 g/L metal concentrates of waste printed circuit boards. The results of bioleaching experiments showed that 99.3% copper could be mobilized from metal concentrates in 62 h under the conditions of initial pH of 2.25, 9 g/L of initial Fe2+, 10% of inoculation quantity, 15 g/L of metal concentrates. Similarly, the filter-sterilized solution could leach 96.0% copper from metal concentrates in 86 h. However, the copper mobilization efficiency of abiotic leaching using only 9K liquid medium without inoculating strain Z1 was just 61.3% at 134 h. Therefore, the isolated strain Z1 can be used as a potential bacterium to bioleach metals from waste printed circuit boards.
- Acidithiobacillus ferrooxidans,
- waste printed circuit boards,
- metal concentrates,
- bioleaching,
- copper

References

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Isolation of an Acidithiobacillus ferrooxidans strain and its performance in bioleaching of copper from waste printed circuit boards

Received Date: 02/04/2012

Accepted Date: 22/02/2012

Available Online: 09/04/2013

Fund Project:

Key words:

Abstract: An acidophilic bacterium named as Z1 was isolated from the acid mine drainage of a pyrite mine in Guangdong. It was identified as Acidithiobacillus ferrooxidans (A. ferrooxidans) genus by morphology, physiology and near full-length 16S rRNA gene analysis. The strain Z1 and the strains such as A. ferrooxidans strain CMS in GenBank were in the same branch of the phylogenetic tree. The 16S rRNA gene sequence of the strain Z1 had 99% sequence similarity to A. ferrooxidans strain CMS. The optimum growth pH value and temperature, and the logarithmic phase of strain Z1 were 2.25 and 30℃, and from 18 h to 30 h, respectively. The average ferrous oxidation rate of the Z1 reached 0.2307 g/(L·h). The adapted strain Z1 could tolerate 15 g/L metal concentrates of waste printed circuit boards. The results of bioleaching experiments showed that 99.3% copper could be mobilized from metal concentrates in 62 h under the conditions of initial pH of 2.25, 9 g/L of initial Fe2+, 10% of inoculation quantity, 15 g/L of metal concentrates. Similarly, the filter-sterilized solution could leach 96.0% copper from metal concentrates in 86 h. However, the copper mobilization efficiency of abiotic leaching using only 9K liquid medium without inoculating strain Z1 was just 61.3% at 134 h. Therefore, the isolated strain Z1 can be used as a potential bacterium to bioleach metals from waste printed circuit boards.

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Isolation of an Acidithiobacillus ferrooxidans strain and its performance in bioleaching of copper from waste printed circuit boards

Abstract

References

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通讯作者: 陈斌, bchen63@163.com

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