Yu Wenjuan, Xie Xuehui, Hong Wulin, Liu Jianshe. Kinetics and optimizing immobilization conditions for strain RB5-M1 decolorizing Reactive Black 5[J]. Chinese Journal of Environmental Engineering, 2013, 7(3): 1175-1180.
Citation: Yu Wenjuan, Xie Xuehui, Hong Wulin, Liu Jianshe. Kinetics and optimizing immobilization conditions for strain RB5-M1 decolorizing Reactive Black 5[J]. Chinese Journal of Environmental Engineering, 2013, 7(3): 1175-1180.

Kinetics and optimizing immobilization conditions for strain RB5-M1 decolorizing Reactive Black 5

  • Received Date: 29/12/2011
    Accepted Date: 10/11/2011
    Available Online: 18/03/2013
    Fund Project:
  • Based on experiments of Aeromonas hydrophila strain RB5-M1 decolorizing Reactive Black 5, the kinetic equations at different initial dye concentrations were discussed. Furthermore, the optimized immobilization conditions were concluded by considering three factors, respectively the mechanical strength, imbedding biomass and the leakage biomass. Results showed that when dyes were added at different initial concentrations of 50, 100, 150, 200, 250 and 300 mg/L, the kinetic equation would be highly correspond with the Monod zero-order reaction equation. As for the immobilization condition, of which cooling temperature is 30℃, the ratio of PVA to sodium alginate is 10∶1, concentration of CaCl2 is 2%, concentration of gelatin is 0.2%, then the immobilized globules could form with higher mechanical strength and embedding biomass. Under the condition of which concentration of CaCl2 is 2%; concentration of gelatin is 0.3%, the ratio of PVA to sodium alginate equals to 9∶2, cooling temperature is 20℃, then immobilized globules could form with lower leakage biomass.
  • [1] 柳广飞,周集体,王竞.细菌对偶氮染料的降解及偶氮还原酶的研究进展.环境科学与技术,2006,29(4):112-114 Liu Guangfei,Zhou Jiti,Wang Jing.Progress on degradation of azo dyes by bacteria and azo reeducates.Environmental Science and Technology,2006,29(4):112-114 (in Chinese)

    Google Scholar Pub Med

    [2] Rodrigo Otávio Alves de Lima,Ana Paula Bazo,Daisy Maria Fávero Salvadori,et al.Mutagenic and carcinogenic potential of a textile azo dye processing plant effluent that impacts a drinking water source.Mutation Research/Genetic Toxicology and Environmental Mutagenesis,2007,626(1-2):53-60

    Google Scholar Pub Med

    [3] 钟金汤.偶氮染料及其代谢产物的化学结构与毒性关系的回顾与前瞻.环境与职业医学,2004,21(1):58-61 King-Thom Chung.Review of the mutagenicity,carcinogenicity and autoimmunity of azo dyes and their metabolites from the perspective of their structure-activity relationships.J. Environ. Occup. Med.,2004,21(1):58-61 (in Chinese)

    Google Scholar Pub Med

    [4] Jun Wu,Liguan Li,Hongwei Du.Biodegradation of leuco derivatives of triphenylmethane dyes by Sphingomonas sp.CM9.Biodegradation,2010,22(5):897-904

    Google Scholar Pub Med

    [5] Chen-Yeon Chu, Shu-Yii Wu, Po-Chi Hsieh.Biohydrogen production from immobilized cells and suspended sludge systems with condensed molasses fermentation solubles.International Journal of Hydrogen Energy,2011,36(21):14078-14085

    Google Scholar Pub Med

    [6] 王娜,闵小波,王云燕.内聚营养源SRB固定化交联剂的选择及对含锌废水的处理.中南大学学报(自然科学版),2008,39(2):273-278 Wang Na,Min Xiaobo,Wang Yunyan.Determination of crosslinking agent in immobilized beads of SRB sludge with inner cohesive carbon source and treatment of wastewater containing zinc.J.Cent.South Univ.(Science and Technology),2008,39(2):273-278(in Chinese)

    Google Scholar Pub Med

    [7] 王建龙,施汉昌.聚乙烯醇包埋固定化微生物的研究及进展.工业微生物,1998,28(2):35-39 Wang Jianlong,Shi Hanchang.PVA immobilization of microbial research and progress.Industrial Microbiology,1998,28(2):35-39(in Chinese)

    Google Scholar Pub Med

    [8] Sulaiman Al-Zuhair,Muftah El-Naas.Immobilization of Pseudomonas putida in PVA gel particles for the biodegradation of phenol at high concentrations.Biochemical Engineering Journal,2011,56(1-2):46-50

    Google Scholar Pub Med

    [9] Zain N.A.M.,Suhaimi M.S.,Idris A.Development and modification of PVA-alginate as a suitable immobilization matrix.Process Biochemistry,2011,46(11):2122-2129

    Google Scholar Pub Med

    [10] Ju X.,Pan J.,Yu H.L.,et al.Improving Pseudomonas sp.esterase performance by engineering approaches for kinetic resolution of 2-acetoxyphenylacetic acids.Biochemical Engineering Journal,2011,57 (1):63-68

    Google Scholar Pub Med

    [11] Kanugula A.K.,Repalle E.R.,Pandey J.P.,et al.Immobilization of organophosphate hydrolase on biocompatible gelatin pads and its use in removal of organophosphate compounds and nerve agents.Indian Journal of Biochemistry and Biophysics,2011,48 (1):29-34

    Google Scholar Pub Med

    [12] 普利锋,赵刚,王建龙.几种交联剂对PVA 固定化活性污泥的活性比较.清华大学学报(自然科学版),2004,44 (12):1603-1605 Pu Lifeng,Zhao Gang,Wang Jianlong.Comparison of microbial activity of activated sludge immobilized in polyvinyl alcohol gel by various reagents.J. Tsinghua Univ. (Sci. & Tech.),2004,44 (12):1603-1605 (in Chinese)

    Google Scholar Pub Med

    [13] 李博.固定化Citrobacter sp.CK3对活性红偶氮染料的脱色研究.厦门:厦门大学硕士学位论文,2009 Li Bo.Decolorization of reactive red azo dyes by immobilized Citrobacter sp.CK3.Xiamen:Master Dissertation of Xiamen University,2009 (in Chinese)

    Google Scholar Pub Med

    [14] 李峰,吕锡武,严伟.聚乙烯醇作为固定化细胞包埋剂的研究.中国给水排水,2000,16(12):14-17 Li Feng,Lü Xiwu,Yan Wei.Study on polyvinyl alcohol (PVA) entrapping agents for immobilizing microbial cell.China Water & Wastewater,2000,16(12):14-17(in Chinese)

    Google Scholar Pub Med

    [15] 贺江,樊明涛,包飞.固定化酵母粒子的制备及特性.酿酒科技,2007,(11):29-33 He Jiang,Fan Mingtao,Bao Fei.Preparation of immobilized yeast granule and its properties.Liquor-Making Science & Technology,2007,(11):29-33(in Chinese)

    Google Scholar Pub Med

    [16] 尚晓冬,李明容,王南,等.应用血球计数板检测灵芝破壁孢子粉破壁率的研究.食用菌学报,2005,12 (2):37-40 Shang Xiaodong,Li Mingrong,Wang Nan,et al.Determination of sporoderm-broken rate of ganoderma lucidum by hemocytometer measurement.Acta Edulis Fungi,2005,12 (2):37-40(in Chinese)

    Google Scholar Pub Med

    [17] Tomei M.C.,Annesini M.C.Biodegradation of phenolic mixtures in a sequencing batch reactor:A kinetic study.Environmental Science and Pollution Research,2008,15 (3):188-195

    Google Scholar Pub Med

    [18] 聂麦茜,吴蔓莉,王晓昌,等.一株黄杆菌及其粗酶液对芘降解的动力学研究.环境科学学报,2006,26(2):181-185 Nie Maiqian,Wu Manli,Wang Xiaochang,et al.The study of kinetic properties on degradation of pyrene by a flavobacterium sp.and its enzyme .Acta Scientiae Circumstantiae,2006,26(2):181-185(in Chinese)

    Google Scholar Pub Med

    [19] 邱丽娟,黄满红,杨煜东.活性黑KN-B染料的生物降解研究.环境科学与技术,2010,33(6):63-66 Qiu Lijuan,Huang Manhong,Yang Yudong,et al.Study on biodegradation of reactive black KN-B.Environmental Science & Technology,2010,33(6):63-66(in Chinese)

    Google Scholar Pub Med

    [20] 刘树文,李华.改善固定化微生物细胞粒子机械强度的研究.微生物学杂志,2005,25(4):32-34 Liu Shuwen,Li Hua.Mechanical strength improvement of immobil ized microbial cell granule.Journal of Microbiology,2005,25(4):32-34(in Chinese)

    Google Scholar Pub Med

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Kinetics and optimizing immobilization conditions for strain RB5-M1 decolorizing Reactive Black 5

Fund Project:

Abstract: Based on experiments of Aeromonas hydrophila strain RB5-M1 decolorizing Reactive Black 5, the kinetic equations at different initial dye concentrations were discussed. Furthermore, the optimized immobilization conditions were concluded by considering three factors, respectively the mechanical strength, imbedding biomass and the leakage biomass. Results showed that when dyes were added at different initial concentrations of 50, 100, 150, 200, 250 and 300 mg/L, the kinetic equation would be highly correspond with the Monod zero-order reaction equation. As for the immobilization condition, of which cooling temperature is 30℃, the ratio of PVA to sodium alginate is 10∶1, concentration of CaCl2 is 2%, concentration of gelatin is 0.2%, then the immobilized globules could form with higher mechanical strength and embedding biomass. Under the condition of which concentration of CaCl2 is 2%; concentration of gelatin is 0.3%, the ratio of PVA to sodium alginate equals to 9∶2, cooling temperature is 20℃, then immobilized globules could form with lower leakage biomass.

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