2024 Volume 19 Issue 6
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ZHUANG Jiyang, WU Huizhen, QIAN Mingrong, JIN Zhexiong, SHENG Yingfei, ZHANG Hu, WANG Junliang, SUN Nabo. The Stress Effects of Difenoconazole and Chiral Difenoconazole-alcohol on Selenastrum capricornutum[J]. Asian journal of ecotoxicology, 2024, 19(6): 335-343. doi: 10.7524/AJE.1673-5897.20240618001
Citation: ZHUANG Jiyang, WU Huizhen, QIAN Mingrong, JIN Zhexiong, SHENG Yingfei, ZHANG Hu, WANG Junliang, SUN Nabo. The Stress Effects of Difenoconazole and Chiral Difenoconazole-alcohol on Selenastrum capricornutum[J]. Asian journal of ecotoxicology, 2024, 19(6): 335-343. doi: 10.7524/AJE.1673-5897.20240618001
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The Stress Effects of Difenoconazole and Chiral Difenoconazole-alcohol on Selenastrum capricornutum

  • 1. College of Environment, Zhejiang University of Technology, Hangzhou 310032, China;

  • 2. College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China;

  • 3. Interdisciplinary Research Academy, Zhejiang Shuren University, Hangzhou 310015, China;

  • 4. Greentown Agricultural Science Testing Technology Co. Ltd., Hangzhou 310052, China;

  • 5. Shaoxing Research Institute, Zhejiang University of Technology, Shaoxing 312000, China

  • Corresponding authors: WANG Junliang ;  SUN Nabo
  • Received Date: 18/06/2024
    Accepted Date: 05/08/2024
    Fund Project:
  • This study evaluated the biological toxicity of difenoconazole and its degradation product difenoconazole-alcohol to Selenastrum capricornutum by measuring median effective concentration (EC50), photosynthetic pigment levels, malondialdehyde (MDA) content, and antioxidant enzyme activity, with a focus on analyzing the stereoselective differences in chiral difenoconazole-alcohol’s biological toxicity to algae. The EC50 values of difenoconazole and difenoconazole-alcohol after 96 hours exposure were 0.67 mg·L-1 and 1.74 mg·L-1, respectively. The EC50 values of R-(+)- difenoconazole-alcohol and S-(-)-difenoconazole-alcohol after 96 hours exposure were 2.74 mg·L-1 and 1.68 mg·L-1, respectively. At the same time, based on the evaluation of changing the trend of oxidative damage-related parameters the levels of chlorophyll content of photosynthesis carriers as the algae was subjected to exposure stress by difenoconazole, difenoconazole-alcohol, and difenoconazole-alcohol enantiomer, it was concluded that difenoconazole was more toxic to Selenastrum capricornutum than difenoconazole-alcohol, and the toxicity of chiral difenoconazole-alcohol was regarded as S-(-)-enantiomer> racemate> R-(+)-enantiomer.
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The Stress Effects of Difenoconazole and Chiral Difenoconazole-alcohol on Selenastrum capricornutum

    Corresponding author: WANG Junliang ; 
    Corresponding authors: SUN Nabo
  • Received Date: 18/06/2024
  • Accepted Date: 05/08/2024
Fund Project:

Abstract: This study evaluated the biological toxicity of difenoconazole and its degradation product difenoconazole-alcohol to Selenastrum capricornutum by measuring median effective concentration (EC50), photosynthetic pigment levels, malondialdehyde (MDA) content, and antioxidant enzyme activity, with a focus on analyzing the stereoselective differences in chiral difenoconazole-alcohol’s biological toxicity to algae. The EC50 values of difenoconazole and difenoconazole-alcohol after 96 hours exposure were 0.67 mg·L-1 and 1.74 mg·L-1, respectively. The EC50 values of R-(+)- difenoconazole-alcohol and S-(-)-difenoconazole-alcohol after 96 hours exposure were 2.74 mg·L-1 and 1.68 mg·L-1, respectively. At the same time, based on the evaluation of changing the trend of oxidative damage-related parameters the levels of chlorophyll content of photosynthesis carriers as the algae was subjected to exposure stress by difenoconazole, difenoconazole-alcohol, and difenoconazole-alcohol enantiomer, it was concluded that difenoconazole was more toxic to Selenastrum capricornutum than difenoconazole-alcohol, and the toxicity of chiral difenoconazole-alcohol was regarded as S-(-)-enantiomer> racemate> R-(+)-enantiomer.

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