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GUO Xinyuan, QI Guangxia, WANG Yongjing, REN Lianhai. Preparation of liquid bacterial fertilizer of nitrogen-fixing bacteria from food waste-recycling wastewater[J]. Chinese Journal of Environmental Engineering, 2017, 11(9): 4978-4984. doi: 10.12030/j.cjee.201609166
Citation: GUO Xinyuan, QI Guangxia, WANG Yongjing, REN Lianhai. Preparation of liquid bacterial fertilizer of nitrogen-fixing bacteria from food waste-recycling wastewater[J]. Chinese Journal of Environmental Engineering, 2017, 11(9): 4978-4984. doi: 10.12030/j.cjee.201609166
shu

Preparation of liquid bacterial fertilizer of nitrogen-fixing bacteria from food waste-recycling wastewater

  • 1.

    College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100022, China

  • 2.

    Department of Environmental Science and Engineering, Beijing Technology and Business University, Beijing 100048, China

  • Received Date: 21/11/2016
    Accepted Date: 19/09/2016
    Available Online: 26/08/2017
    Fund Project:
  • The feasibility of using food waste-recycling wastewater as fermentation substrate for producing liquid Azotobacter chroococcum fertilizer was investigated in this study. The results showed that Azotobacter chroococcum cultured in the food waste-recycling wastewater reached logarithmic growth stage in as short as 3 days of adaptation period, and the number of strains reached the maxima on the 4th to 5th day(wastewater Ⅰ 3.0×1012 CFU·mL-1,wastewater Ⅱ 3.3×1012 CFU·mL-1). The promotion effect of food waste hydrothermal treatment on the growth of Azotobacter chroococcum was very limited. pH and salt concentration of the wastewater had great influence on the growth and metabolism of Azotobacter chroococcum. The optimal pH for Azotobacter chroococcum growth in wastewaterⅠwas pH 7, in wastewaterⅡwas pH 7.5,while other pH would inhibit the growth and metabolism activity of the nitrogen-fixing bacteria. With an increase of NaCl concentration, the strain numbers first increased and then steeply decreased, and the optimal NaCl concentration for bacterial culture was only 3 g·L-1. The variation of temperature, shaking speed and strain inoculation volume affected the growth of Azotobacter chroococcum significantly. The optimal culture conditions were pH=7, T=28℃, shaking speed 150 r·min-1, and inoculums of 2%(volume fraction) as determined by orthogonal experiment. The nitrogen-fixing bacterial fertilizer prepared from food waste-recycling wastewater was able to rise the total nitrogen concentration of soil (linear correlation R2=0.979), and the dry weight of soy beans grown in soils amended with 0.025‰ to 2.5‰ of nitrogen-fixing bacterial fertilizer was from 55.2% to 67.2% of that grown in the soil amended with 5‰ of inorganic fertilizer on weight basis.
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Preparation of liquid bacterial fertilizer of nitrogen-fixing bacteria from food waste-recycling wastewater

  • Received Date: 21/11/2016
  • Accepted Date: 19/09/2016
  • Available Online: 26/08/2017
Fund Project:

Abstract: The feasibility of using food waste-recycling wastewater as fermentation substrate for producing liquid Azotobacter chroococcum fertilizer was investigated in this study. The results showed that Azotobacter chroococcum cultured in the food waste-recycling wastewater reached logarithmic growth stage in as short as 3 days of adaptation period, and the number of strains reached the maxima on the 4th to 5th day(wastewater Ⅰ 3.0×1012 CFU·mL-1,wastewater Ⅱ 3.3×1012 CFU·mL-1). The promotion effect of food waste hydrothermal treatment on the growth of Azotobacter chroococcum was very limited. pH and salt concentration of the wastewater had great influence on the growth and metabolism of Azotobacter chroococcum. The optimal pH for Azotobacter chroococcum growth in wastewaterⅠwas pH 7, in wastewaterⅡwas pH 7.5,while other pH would inhibit the growth and metabolism activity of the nitrogen-fixing bacteria. With an increase of NaCl concentration, the strain numbers first increased and then steeply decreased, and the optimal NaCl concentration for bacterial culture was only 3 g·L-1. The variation of temperature, shaking speed and strain inoculation volume affected the growth of Azotobacter chroococcum significantly. The optimal culture conditions were pH=7, T=28℃, shaking speed 150 r·min-1, and inoculums of 2%(volume fraction) as determined by orthogonal experiment. The nitrogen-fixing bacterial fertilizer prepared from food waste-recycling wastewater was able to rise the total nitrogen concentration of soil (linear correlation R2=0.979), and the dry weight of soy beans grown in soils amended with 0.025‰ to 2.5‰ of nitrogen-fixing bacterial fertilizer was from 55.2% to 67.2% of that grown in the soil amended with 5‰ of inorganic fertilizer on weight basis.

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