不同实验条件下稀有鮈鲫胚胎的自然受精率和孵化率
Natural Fertility and Hatchability of Gobiocypris rarus Embryos under Different Experimental Conditions
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摘要: 以中国本土鱼种稀有鮈鲫（Gobiocypris rarus）胚胎为实验生物，研究稀有鮈鲫胚胎在正常光照条件下（温度（26±1）℃，自然光照）和光照周期颠倒条件下（温度（26±1）℃，光照周期为12 h（暗）：12 h（光））的96 h自然受精率和孵化率，并在放大倍数为40倍的体视显微镜下观察稀有鮈鲫在胚胎发育过程中的形态发育时序及特征。结果表明，在光照周期颠倒和（26±1）℃条件下，9次重复试验的稀有鮈鲫胚胎自然受精率和孵化率平均值分别为92.2%和92.8%，变异系数（CV）分别为5.65%和6.21%，比正常光照和（26±1）℃条件下稀有鮈鲫胚胎的自然受精率和孵化率更高，为鱼类胚胎试验提供更高质量的胚胎。稀有鮈鲫胚胎的形态发育时序及特征满足胚胎试验观察的需求，可在特定的时间点观察特定表征。Abstract: Two groups of eggs of Gobiocypris rarus (a Chinese native species) were bred for 96 hours at the same controlled water temperature (26℃±1℃) but different illumination conditions (natural vs. reversed photoperiods). Fertilization rate of the eggs and hatching rate in both groups were recorded, and the morphogenetic development sequence and characteristics of Gobiocypris rarus embryos were observed by means of a stereoscopic microscope at 40-fold magnification. It was found that the means of natural fertilization rate and hatching rate of Gobiocypris rarus embryos under a reversed illumination condition from the nature light cycle in nine repeated tests were 92.2% and 92.8% with the coefficient of variation (CV) of 5.65% and 6.21%, respectively, higher than those under normal illumination conditions, implying that embryos of higher quality to meet test requirements for fish embryo testing can be obtained by controlling photoperiod. There was no difference in the morphogenetic development sequence and characteristics of Gobiocypris rarus embryos between the two groups, and therefore, a specific development stage could be achieved at a desired time point to facilitate the testing performance.
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Key words:
- Gobiocypris rarus /
- natural fertility /
- hatchability /
- normal illumination /
- illumination cycle reversal
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Davies G, Greenhough B, Hobson-West P, et al. Recovering The Principles of Humane Experimental Technique[J]. Science, Technology and Human Values, 2018, 43(4):622-648 Michelle R E, Scott E B, Thomas B, et al. The fish embryo toxicity test as an animal alternative method in hazard and risk assessment and scientific research[J]. Aquatic Toxicology, 2010, 97(2):79-87 曹文宣, 王剑伟. 稀有鮈鲫——一种新的鱼类实验动物[J]. 实验动物科学与管理, 2003, 20(z1):96-99 Cao W X, Wang J W. Rare minnow:A new laboratory animal in China[J]. Laboratory Animal Science and Management, 2003, 20(zl):96-99(in Chinese)
王蕊, 殷浩文. 斑马鱼胚胎发育中适宜的毒理学指标分析[J]. 环境与职业医学, 2004, 21(2):88-89 , 93 Wang R, Yin H W. Analysis on feasible toxicologic endpoints in embryonic development of Brachydanio rerio[J]. Journal of Environmental and Occupational Medicine, 2004, 21(2):88-89, 93(in Chinese)
朱晓鸣, 解绶启, 崔奕波, 等. 摄食水平和性别对稀有鮈鲫生长和能量收支的影响[J]. 海洋与湖沼, 2001, 32(3):240-247 Zhu X M, Xie S Q, Cui Y B, et al. Effect of ration level on growth and energy budget of male and female rare minnow Gobiocypris rarus[J]. Oceanologia et Limnologia Sinica, 2001, 32(3):240-247(in Chinese)
王剑伟, 曹文宣. 中国本土鱼类模式生物稀有鮈鲫研究应用的历史与现状[J]. 生态毒理学报, 2017, 12(2):20-33 Wang J W, Cao W X. Gobiocypris rarus as a Chinese native model organism:History and current situation[J]. Asian Journal of Ecotoxicology, 2017, 12(2):20-33(in Chinese)
罗思. 主要饲养环境因子对稀有鮈鲫生长、发育和繁殖的影响[D]. 北京:中国科学院大学, 2016:23-36 Luo S. Effect of main breeding environment factors on the growth, development and reproduction of Gobiocypris rarus[D]. Beijing:University of Chinese Academy of Sciences, 2016:23 -36(in Chinese)
王剑伟. 稀有鮈鲫的繁殖生物学[J]. 水生生物学报, 1992, 16(2):165-174 Wang J W. Reproductive biology of Gobiocypris rarus[J]. Acta Hydrobiologica Sinica, 1992, 16(2):165-174(in Chinese)
常剑波, 王剑伟, 曹文宣. 稀有鮈鲫胚胎发育研究[J]. 水生生物学报, 1995, 19(2):97-103 Chang J B, Wang J W, Cao W X. The embryonic development of Gobiocypris rarus[J]. Acta Hydrobiologica Sinica, 1995, 19(2):97-103(in Chinese)
Organization for Economic Co-operation and Development (OECD). OECD Guidelines for Testing of Chemicals, 236 Fish Embryo Acute Toxicity (FET) Test[S]. Paris:OECD, 2013 Organization for Economic Co-operation and Development (OECD). OECD Guidelines for Testing of Chemicals, 210 Fish Early-life Stage Toxicity Test[S]. Paris:OECD, 2013 Organization for Economic Co-operation and Development (OECD). OECD Guidelines for Testing of Chemicals, 212 Fish, Short-term Toxicity Test on Embryo and Sac-fry Stages[S]. Paris:OECD, 1998 王剑伟, 宋天祥, 曹文宣. 稀有鮈鲫胚后发育和幼鱼生长的初步研究[J]. 水生生物学报, 1998, 22(2):128-134 Wang J W, Song T X, Cao W X. Postembryonic development and growth of cultured rare minnow, Gobiocypris rarus[J]. Acta Hydrobiologica Sinica, 1998, 22(2):128-134(in Chinese)
吴本丽. 稀有鮈鲫对饲料中主要营养素的需求及饲养策略的研究[D]. 北京:中国科学院大学, 2015:15-27 Wu B L.Study on the demand of main nutrients in feed and feeding strategy for Gobiocypris rarus[D]. Beijing:University of Chinese Academy of Sciences, 2015:15 -27(in Chinese)
Luo S, Wu B L, Xiong X Q, et al. Effects of total hardness and calcium:Magnesium ratio of water during early stages of rare minnows (Gobiocypris rarus)[J]. Comparative Medicine, 2016, 66(3):181-187 李忻林, 董洪坪, 王婷, 等. 温度对斑马鱼胚胎发育的影响[J]. 四川动物, 2014, 33(6):829-835 Li X L, Dong H P, Wang T, et al. Effect of temperature on embryonic development of zebrafish[J]. Sichuan Journal of Zoology, 2014, 33(6):829-835(in Chinese)
Organization for Economic Co-operation and Development (OECD). Validation Report (Phase 1) for the Zebrafish Embryo Toxicity Test. Series on Testing and Assessment, No. 157[R]. Paris:OECD, 2011 Organization for Economic Co-operation and Development (OECD). Validation Report (Phase 2) for the Zebrafish Embryo Toxicity Test. Series on Testing and Assessment, No. 179[R]. Paris:OECD, 2012 -
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