摘要:
为了研究运动对2,3,7,8-四氯二苯并二恶英(2,3,7,8-TCDD)急性暴露大鼠肝组织酶活性的影响,将40只雄性Wistar大鼠随机分为正常对照组(NC)、染毒组(NT)、运动对照组(EC)、运动染毒组(ET)。染毒组(NT组与ET组)腹腔注射10μg·kg-1(以单位体重计)的TCDD,对照组(NC组与EC组)腹腔注射等量的玉米油;NT、NC组静养4周,ET、EC组运动(尾部负重5%游泳30分钟)4周。4周后,称重并宰杀大鼠,分离肝组织,称重后-80℃保存待测7-乙氧基异吩恶唑酮脱乙基酶(EROD)、7-乙氧基香豆素-O-脱乙基酶(ECOD)及芳香烃羟化酶(AHH)的活性。将数据进行多因素方差分析(MAVONA)处理,结果表明,染毒可降低大鼠体重,增加肝湿重和肝相对重量、增加EROD、ECOD活性;运动可增加大鼠肝相对重量、增加AHH的活性;染毒后运动可降低EROD、ECOD的活性。结论:急性10μg·kg-1(以单位体重计)TCDD染毒后4周可增加大鼠肝相对重量;4周的运动能有效降低TCDD对EROD、ECOD活性的激活作用。
Abstract:
The aim of the present study was to investigate the effects of exercise on the activity of liver microsomal oxidase in acute 2,3,7,8-TCDD-exposed rats. Forty male Wistar rats were randomly divided into four groups:normal control (NC), normal toxic (NT), exercise control (EC), and exercise toxic (ET). The rats in NC and ET groups were intmperitoneal injection of 2,3,7,8-TCDD (10 μg·kg-1); the NC and EC animals were administered with equivalent volume of coin oil, and the rats in exercise groups were submitted to swimming for 30 min.d-1 with 5% weights attached to tails. After 4 weeks, the rats were sacrificed and the livers were imme-diately isolated and weighted. The lobe of each liver was kept at -80℃ for detecting the activity of hepatic microsomal enzymes. The results were statistically analyzed using MANOVA. A decreased body weight together with increased liver weight, relative liver weight, and the activities of 7-ethoxyresomfm O-deethylase (EROD) and 7-ethoxycoumarin O-deethylase (ECOD) were observed in toxic group; an increased relative liver weight and the activity of aryl hydrocarbon hydroxylase (AHH) were observed in exercise group; and exercise could effectively prevent the activation of 2,3,7,8-TCDD on the activities of EROD and ECOD. The results suggest that acute exposure to 2,3,7,8-TCDD can increase the relative liver weight, and exercise can effectively prevent the activation of 2,3,7,8-TCDD on the activities of EROD and ECOD.
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