敌百虫致小鼠外周血淋巴细胞DNA-蛋白质交联作用研究
- 1. 贵州大学动物科学学院高原山地动物遗传育种与繁殖省部共建教育部重点实验室;
- 2. 贵阳市花溪区农业局
摘要: 为探讨敌百虫致DNA-蛋白质交联作用,以昆明小鼠为受试动物,敌百虫按0、20、40、60 mg·kg-14个剂量水平,灌胃染毒小鼠2周。第5组以提取的正常小鼠外周血淋巴细胞经50μmol·L-1的H2O2处理为阳性对照组。采用经改进的彗星试验方法来检测染毒后外周血淋巴细胞DNA-蛋白质交联效应。结果表明,与空白组相比,各敌百虫染毒组都能引起DNA损伤作用(P<0.01)并引起一定程度的DNA-蛋白质交联效应,在较高浓度(40、60 mg·kg-1)时DNA-蛋白质交联尤为明显,存在一定的潜在突变风险。
Study on DNA-Protein Crosslinks Induced by Trichlorfon in Peripheral Blood Lymphocyte of Mice
- 1. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region of Ministry of Education and Guizhou Province, College of Animal Science, Guizhou University, Guiyang 550025, China;
- 2. Huaxi Agriculture Bureau of Guiyang, Guiyang 550025, China
Fund Project:
Abstract: In order to study DNA-protein crosslinks(DPC) of trichlorfon on peripheral blood lymphocyte in mice, mice were orally administrated with trichlorfon daily for 14 days at the concentrations of 0, 20, 40, 60 mg·kg-1 respectively. The fifth group as positive control group was established by extracting the peripheral blood in mice treated with 50 μLmol·L-1 hydrogen peroxide. The DPC effects of trichlorfon were measured by comet assay. The results indicated that every group can cause the DNA damaged (P<0.01)and lead to DPC effects compared with the blank group. Moreover, DPC is highly obvious when at high concentrations (40, 60 mg·kg-1), which suggested there was a risk of potential mutation.
点击查看大图
下载:
