摘要:
轻稀土元素进入生物体后主要累积于肝脏,进入肝细胞,分布于细胞核上。为探讨轻稀土元素对小鼠肝细胞核的氧化损伤作用,选用5周龄雄性封闭群(ICR)小鼠灌喂10、20和40 mg·kg-1的稀土元素镧(La)、铈(Ce)和钕(Nd),6周后测定小鼠肝细胞核中超氧化物岐化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GPx)的活性,并测定谷胱甘肽(GSH)和丙二醛(MDA)的含量。将实验数据进行ANOVA分析,结果显示,与对照组相比,各染毒组GPx活性和MDA含量显著升高(p<0.05);除Ce低剂量组外,各染毒组SOD和CAT活性显著降低(p<0.05),且其活性的降低与各稀土元素的暴露剂量有剂量-效应关系;除La低剂量组和Ce低、中剂量组外,各染毒组GSH含量显著升高(p<0.05)。结果表明,稀土元素La、Ce和Nd会造成小鼠肝细胞核的氧化损伤。
Abstract:
Light rare earth elements are found to mainly accumulate in the livers of receiving organisms.They can enter into hepatocytes and are mainly distributed in the nuclei.To investigate the oxidative damage effects of light rare earth elements on the hepatocyte nuclei isolated from the mouse liver,five-week-old male ICR(imprinting control region) mice were exposed to lanthanum(La),cerium(Ce) and neodymium(Nd) by oral gavage for 6 weeks with different doses,i.e.,10,20 and 40 mg·kg-1.The activities of superoxide dismutase(SOD),catalase(CAT) and glutathione peroxidase(GPx) were determined as well as the contents of glutathione(GSH) and malondialdehyde(MDA).ANOVA analysis of the results showed that compared with the control group,GPx activity and MDA content in every exposure group increased(p<0.05);except in the Ce low-dosage group,SOD and CAT activities in other exposure groups decreased(p<0.05) in a dose-dependent manner;except in the La low-dosage,Ce low-dosage and Ce middle-dosage groups,GSH content in other exposure groups increased(p<0.05).It is indicated that La,Ce and Nd could induce oxidative damage in hepatic nuclei of mice.
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