摘要:
为了研究苯并(b)荧蒽这一典型的多环芳烃化合物对水生生物的毒性效应,测定了不同浓度(2.0、10.0和50.0μg·L-1)苯并(b)荧蒽胁迫15 d和清水释放10 d后翡翠贻贝(Perna viridis)内脏团组织中抗氧化酶(CAT、SOD和GPx)活性和MDA含量的变化。结果表明,2.0μg·L-1浓度组中CAT活性呈现出诱导-抑制的规律,在第4天达到峰值,诱导率为81.9%;10.0和50.0μg·L-1浓度组中SOD活性在整个胁迫阶段呈现抑制-诱导-抑制的趋势,第2天时抑制率分别为31.9%和49.8%,第8天时诱导率分别为24.9%和62.3%;10.0和50.0μg·L-1浓度组中GPx活性在整个胁迫阶段呈现抑制-诱导的变化规律,第8天抑制率分别为30.6%和40.6%,15 d时诱导率分别为20.6%和21.3%。随着清水释放实验的进行,2.0和10.0μg·L-1浓度组的翡翠贻贝内脏团抗氧化酶活性逐渐恢复,氧化损伤逐渐降低,但50.0μg·L-1浓度组中翡翠贻贝的SOD和GPx活性10 d后仍显著低于对照组水平。本研究中,翡翠贻贝内脏团抗氧化酶活性的变化,可以反映苯并(b)荧蒽在一定浓度与时间范围内对机体的胁迫程度;而MDA含量与苯并(b)荧蒽存在显著的时间-效应和浓度-效应关系,表明苯并(b)荧蒽能通过氧化损伤途径对机体产生毒性作用。
Abstract:
To study the toxic effects of benzo[b]fluoranthene on the aquatic life,the activities of antioxidant enzymes(CAT,SOD,GPx) and MDA content in visceral masses of green-lipped mussel(Perna viridis) were measured during 15 days exposure to benzo[b]fluoranthene with the concentration of 2.0,10.0 and 50.0 μg·L-1.After benzo[b]fluoranthene exposure,the green-lipped mussels were then transferred to clean seawater to be cultivated for 10 days,and the above four parameters were also analyzed during this period.The results showed that,in 2.0 μg·L-1 treatment group the CAT activity was induced and then inhibited,and its activity reached the peak value after 4 days exposure,with induction rate being 81.9%;in 10.0 and 50.0 μg·L-1 treatment groups the SOD activities were first inhibited,then induced,and again inhibited,and after 2 days exposure the inhibition rates were 31.9% and 49.8%,respectively,while after 8 days exposure the induction rates were 24.9% and 62.3% respectively;in 10.0 and 50.0 μg·L-1 treatment groups GPx activities were inhibited,and then induced,and after 8 days exposure the inhibition rates were 30.6% and 40.6%,respectively,while after 15 days exposure the induction rates were 20.6% and 21.3%,respectively.After being transferred to the clean seawater,the activities of antioxidant enzymes in 2.0 and 10.0 μg·L-1 treatment groups gradually restored to the control levels,but the activities of SOD and GPx in 50.0 μg·L-1 treatment group were still below the control level after 10 days.In this study,it is found that in certain range of concentration and time,the damage to Perna viridis induced by benzo[b]fluoranthene could be reflected by the changes in the activities of SOD,CAT and GPx.Benzo[b]fluoranthene and MDA content showed significant dose-effect and time-effect relationships,which indicated that benzo[b]fluoranthene exerted toxic effects on Perna viridis by way of oxidative damage.
点击查看大图
下载:
