高级搜索

无机碳在厌氧氨氧化系统中的迁移转化

,  , 

downloadPDF

上一篇

下一篇

韦愿, 王华琴, 王敦球. 无机碳在厌氧氨氧化系统中的迁移转化[J]. 环境工程学报, 2019, 13(6): 1322-1328. doi: 10.12030/j.cjee.201809020
引用本文: 韦愿, 王华琴, 王敦球. 无机碳在厌氧氨氧化系统中的迁移转化[J]. 环境工程学报, 2019, 13(6): 1322-1328. doi: 10.12030/j.cjee.201809020
shu
WEI Yuan, WANG Huaqin, WANG Dunqiu. Migration and transformation of inorganic carbon in the Anammox system[J]. Chinese Journal of Environmental Engineering, 2019, 13(6): 1322-1328. doi: 10.12030/j.cjee.201809020
Citation: WEI Yuan, WANG Huaqin, WANG Dunqiu. Migration and transformation of inorganic carbon in the Anammox system[J]. Chinese Journal of Environmental Engineering, 2019, 13(6): 1322-1328. doi: 10.12030/j.cjee.201809020
shu

无机碳在厌氧氨氧化系统中的迁移转化

  • 1.

    桂林理工大学，广西环境污染控制理论与技术重点实验室，桂林 541004

  • 2.

    桂林理工大学，广西岩溶地区水污染控制与用水安全保障协同创新中心，桂林 541004

  • 基金项目:

    国家自然科学基金资助项目51668013，51638006

    广西科技计划项目资助桂科AD18126018

    桂林市科学研究与技术开发计划项目2016012303

    广西矿冶与环境科学实验中心KH2012ZD004

    广西高等学校高水平创新团队及卓越学者计划项目002401013001国家自然科学基金资助项目(51668013，51638006)

    “八桂学者”建设工程专项经费

    广西科技计划项目资助(桂科AD18126018)

    桂林市科学研究与技术开发计划项目(2016012303)

    广西矿冶与环境科学实验中心(KH2012ZD004)

    广西高等学校高水平创新团队及卓越学者计划项目(002401013001)

Migration and transformation of inorganic carbon in the Anammox system

  • 1.

    Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin 541004, China

  • 2.

    Guangxi Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area, Guilin University of Technology, Guilin 541004, China

  • Fund Project:

  • 摘要: 基于厌氧氨氧化(Anammox)反应，采用13C同位素示踪法分析无机碳(IC)在工艺中的迁移转化路径，考查厌氧氨氧化工艺的固碳潜力及厌氧氨氧化菌相关的固碳机理；同时，结合微生物分子学等方法，通过比较反应前后NH4+-N、NO2--N、TN及IC的变化，分析推导出工艺的固碳机理。结果表明，在进水IC为10.70 mg左右时，系统平均固碳率在12.05%以上；经13C标记处理后的Anammox污泥中13C丰度值由1.07%增加至1.17%以上；Anammox污泥中cbbLR1基因拷贝数经氮素和IC影响后分别为5.79×108 copies·g-1和5.56×108 copies·g-1，较处理前均有所增加，但变化不明显。进水中投加的IC参与了微生物体内的碳代谢；厌氧氨氧化菌存在遵循卡尔文循环固碳途径的功能基因。cbbLR1基因丰度与氮素浓度之间呈显著相关，与IC浓度之间的相关性不明显，说明该基因丰度对氮素的响应度比IC大。
    Abstract: In this study, the migration and transformation path of inorganic carbon (IC) in Anammox process was identified using the 13C isotope tracer method, and the carbon sequestration potential of Anammox process and the carbon sequestration mechanism of Anammox bacteria were discussed. At the same time, in combination with the microbial molecular methods, the carbon sequestration mechanism of the process was disclosed through comparison of the changes of NH4+-N, NO2--N, TN and IC before and after the reaction. The experimental results showed that the average carbon sequestration rate of the system was above 12.05% when the influent IC was about 10.70 mg. The 13C abundance value of the anaerobic ammonium oxide sludge after 13C labeling treatment increased from 1.07% to over 1.17%. Under the influence of nitrogen and IC, the copy numbers of cbbLR1 gene in Anammox sludge were 5.79×108 copies·g-1 and 5.56×108 copies·g-1 dry sludge, respectively, both of them increased than before treatment, while this change was insignificant. IC added to the influent water participates in the carbon metabolism in the microorganism. Anammox bacteria have some functional genes that follow the Calvin cycle carbon sequestration pathway. The abundance of cbbLR1 gene showed a significant correlation with nitrogen concentration, while an insignificant correlation with IC concentration, indicating that the gene abundance was more responsive to nitrogen than to IC.
  • 加载中
  • [1] KUENEN J G. Anammox bacteria: From discovery to application[J]. Nature Reviews Microbiology, 2008, 6(4): 320-326.
    [2] STROUS M, KUENEN J G, JETTEN M S M. Key physiology of anaerobic ammonium oxidation[J]. Applied and Environmental Microbiology, 1999, 65(7): 3248-3250.
    [3] MULDERA, GRAFF L A VAN DE, ROBERTSON L A, et al. Anaerobic ammonium oxidation discovered in a denitrifying fluidized bed reactor[J]. FEMS Microbiology Ecology, 1995, 16(3): 177-183.
    [4] KUENEN J G, JETTEN M S M, STROUS M. New concepts of microbial treatment processes for the nitrogen removal in wastewaters[J]. FEMS Microbiology Reviews, 2003, 27(4): 481-492.
    [5] KARTAL B, RATTRAY J, NIFTRIK L A VAN, et al. Candidatus Anammox oglobus propionicus a new propionate oxidizing species of anaerobic ammonium oxidizing bacteria[J]. Systematic and Applied Microbiology, 2007, 30(1): 39-49.
    [6] 丁敏, 黄勇, 袁怡，等. HCO3-浓度对厌氧氨氧化反应的影响[J]. 工业水处理 2015, 35(10): 35-38.
    [7] 朱彤, 梁启煜, 谢元华, 等. 厌氧氨氧化过程中无机碳对脱氮效能的影响[J]. 2018, 39(2): 278-287.
    [8] 鲍林林, 茹祥莉, 郭守德, 等. 无机碳对厌氧氨氧化反应的影响[J]. 河南师范大学学报(自然科学版), 2013, 41(4): 90-93.
    [9] REARDON D J, FALK M W. Sustainability best practices to significantly reduce greenhouse gas and energy at your wastewater facility[J]. Proceedings of the Water Environment Federation, 2010, 13: 3347-3358.
    [10] HAO X D, LIU R B, HUANG X. Evaluation of the potential for operating carbon neutral WWTPs in China[J]. Water Research, 2015, 87: 424-431.
    [11] NANBA K, KING G M, DUNFIELD K. Analysis of facultative lithotrophic distribution and diversity on volcanic deposits by use of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase[J]. Applied and Environmental Microbiology, 2004, 70: 2245-2253.
    [12] XU H H, TABITA F, Ribulose- R.1,5-bisphosphate carboxylase/oxygenase gene expression and diversity of Lake Eric planktonic microorganisms[J]. Applied and Environmental Microbiology,1996, 62(6): 1913-1921.
    [13] ELSAIED H, NAGANUMA T. Phylogenetic diversity of ribulose-1,5-bisphosphate carboxylase/oxygenase large-subunit genes from deep-sea microorganisms[J]. Applied and Environmental Microbiology, 2001, 67: 1751-1765.
    [14] CALVIN M E. The path of carbon in photo synthesis[J]. Journal of Biological Chemistry, 1950, 185(2): 781-788.
    [15] RAGSDALE S W. Enzymology of the acetyl-CoA pathway of CO2 fixation[J]. Critical Reviews in Biochemistry and Molecular Biology, 1991, 26(3/4): 261-300.
    [16] BUCHANAN B B, Arnon D I. A reverse KREBS cycle in photosynthesis: Consensus at last[J]. Photosynthesis Research, 1990, 24(1): 47-53.
    [17] HERTER S, BUSCH A, FUCHS G. L-malyl-coenzyme A lyase/beta-methylmalyl-coenzyme A lyase from Chloroflexus aurantiacus: A bifunctional enzyme involved in autotrophic CO2 fixation[J]. Journal of Bacteriology, 2002, 184(21): 5999-6006.
    [18] 范宗理. 第五条固碳途径[J]. 自然杂志, 2008, 30(2): 93.
    [19] 国家环境保护总局. 水和废水监测分析方法[M]. 4版. 北京: 中国环境科学出版社, 2002.
    [20] 吴鲜梅, 王敦球， 李亮, 等. 厌氧氨氧化污泥启动EGSB反应器研究[J]. 水处理技术, 2013, 39(2): 57-61.
    [21] JETTEN M S M, CIRPUS I, KARTAL B, et al. 1994-2004: 10 years of research on the anaerobic oxidation of ammonium[J]. Biochemical Society Transactions, 2005, 33: 119-123.
    [22] 谭发, 王敦球, 金樾, 等. 无机碳源对一体化厌氧氨氧化反应器脱氮性能的影响研究[J]. 水处理技术，2016, 42(6): 110-113.
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  3981
  • HTML全文浏览数:  3911
  • PDF下载数:  110
  • 施引文献:  0
出版历程
  • 刊出日期:  2019-06-18
韦愿, 王华琴, 王敦球. 无机碳在厌氧氨氧化系统中的迁移转化[J]. 环境工程学报, 2019, 13(6): 1322-1328. doi: 10.12030/j.cjee.201809020
引用本文: 韦愿, 王华琴, 王敦球. 无机碳在厌氧氨氧化系统中的迁移转化[J]. 环境工程学报, 2019, 13(6): 1322-1328. doi: 10.12030/j.cjee.201809020
shu
WEI Yuan, WANG Huaqin, WANG Dunqiu. Migration and transformation of inorganic carbon in the Anammox system[J]. Chinese Journal of Environmental Engineering, 2019, 13(6): 1322-1328. doi: 10.12030/j.cjee.201809020
Citation: WEI Yuan, WANG Huaqin, WANG Dunqiu. Migration and transformation of inorganic carbon in the Anammox system[J]. Chinese Journal of Environmental Engineering, 2019, 13(6): 1322-1328. doi: 10.12030/j.cjee.201809020
shu

无机碳在厌氧氨氧化系统中的迁移转化

  • 1. 桂林理工大学，广西环境污染控制理论与技术重点实验室，桂林 541004
  • 2. 桂林理工大学，广西岩溶地区水污染控制与用水安全保障协同创新中心，桂林 541004
基金项目:

国家自然科学基金资助项目51668013，51638006

广西科技计划项目资助桂科AD18126018

桂林市科学研究与技术开发计划项目2016012303

广西矿冶与环境科学实验中心KH2012ZD004

广西高等学校高水平创新团队及卓越学者计划项目002401013001国家自然科学基金资助项目(51668013，51638006)

“八桂学者”建设工程专项经费

广西科技计划项目资助(桂科AD18126018)

桂林市科学研究与技术开发计划项目(2016012303)

广西矿冶与环境科学实验中心(KH2012ZD004)

广西高等学校高水平创新团队及卓越学者计划项目(002401013001)

摘要: 基于厌氧氨氧化(Anammox)反应，采用13C同位素示踪法分析无机碳(IC)在工艺中的迁移转化路径，考查厌氧氨氧化工艺的固碳潜力及厌氧氨氧化菌相关的固碳机理；同时，结合微生物分子学等方法，通过比较反应前后NH4+-N、NO2--N、TN及IC的变化，分析推导出工艺的固碳机理。结果表明，在进水IC为10.70 mg左右时，系统平均固碳率在12.05%以上；经13C标记处理后的Anammox污泥中13C丰度值由1.07%增加至1.17%以上；Anammox污泥中cbbLR1基因拷贝数经氮素和IC影响后分别为5.79×108 copies·g-1和5.56×108 copies·g-1，较处理前均有所增加，但变化不明显。进水中投加的IC参与了微生物体内的碳代谢；厌氧氨氧化菌存在遵循卡尔文循环固碳途径的功能基因。cbbLR1基因丰度与氮素浓度之间呈显著相关，与IC浓度之间的相关性不明显，说明该基因丰度对氮素的响应度比IC大。

English Abstract

    全文HTML

参考文献 (22)

返回顶部

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心