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Pseudomonas sp. BS-1发酵产物对土壤中钍的淋洗作用

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王维大, 张达, 韩剑宏, 姜庆宏, 李玉梅, 孙鹏, 张连科. Pseudomonas sp. BS-1发酵产物对土壤中钍的淋洗作用[J]. 环境工程学报, 2018, 12(4): 1198-1207. doi: 10.12030/j.cjee.201712036
引用本文: 王维大, 张达, 韩剑宏, 姜庆宏, 李玉梅, 孙鹏, 张连科. Pseudomonas sp. BS-1发酵产物对土壤中钍的淋洗作用[J]. 环境工程学报, 2018, 12(4): 1198-1207. doi: 10.12030/j.cjee.201712036
shu
WANG Weida, ZHANG Da, HAN Jianhong, JANG Qinghong, LI Yumei, SUN Peng, ZHANG Lianke. Leaching properties of thorium in soil by fermentation products of Pseudomonas sp. BS-1[J]. Chinese Journal of Environmental Engineering, 2018, 12(4): 1198-1207. doi: 10.12030/j.cjee.201712036
Citation: WANG Weida, ZHANG Da, HAN Jianhong, JANG Qinghong, LI Yumei, SUN Peng, ZHANG Lianke. Leaching properties of thorium in soil by fermentation products of Pseudomonas sp. BS-1[J]. Chinese Journal of Environmental Engineering, 2018, 12(4): 1198-1207. doi: 10.12030/j.cjee.201712036
shu

Pseudomonas sp. BS-1发酵产物对土壤中钍的淋洗作用

  • 1.

    内蒙古科技大学能源与环境学院，包头 014010

  • 2.

    华中科技大学环境与市政工程学院，武汉 430074

  • 基金项目:

    内蒙古自然科学基金资助项目（2016BS0511）

    内蒙古科技大学创新基金（2016QDL-B08）

Leaching properties of thorium in soil by fermentation products of Pseudomonas sp. BS-1

  • 1.

    School of Energy and Environment, Inner Mongolia University of Science and Technology, Baotou 014010, China

  • 2.

    School of Environmental and Municipal Engineering, Huazhong University of Science and Technology, Wuhan 430074, China

  • Fund Project:

  • 摘要: 从焦化废水污泥中筛选出一株产生物表面活性剂的菌株，将其命名为BS-1，16S rDNA系统发育学分析确定其属于假单胞菌属（Pesudomonas sp.）。该菌株发酵产物初步鉴定为鼠李糖脂，在其临界胶束浓度为0.56 g·L-1时，可将水溶液的表面张力从76.8 mN·m-1降至38.5 mN·m-1。将菌株发酵产物溶液作为淋洗剂，采用浸提和土柱淋洗的方法研究了BS-1发酵产物对污染土壤中钍的去除效果，结果表明：当pH为10，浓度为3 g·L-1，振荡时间为72 h时，钍的去除率可达70.14%；在柱状动态实验中，当淋洗量达到800 mL时，发酵产物溶液对钍的累积去除量最大为65.03 mg·kg-1，发酵产物溶液对钍的累积去除量明显优于去离子水。对比淋洗后土壤中钍的形态占比可知，残渣态增加10.16%，氧化结合态减少了11.41%。对比淋洗前后土壤中钍的形态含量可知，酸可提取态、氧化结合态、有机结合态分别减少了83.25%、75.22%、60.70%。淋洗后钍的稳定性增强。
    Abstract: A biosurfactant-producing strain BS-1 was isolated from sludge in coking wastewater. It was identified as Pseudomonas (Pesudomonas sp.) according to 16S rDNA phylogenetic analysis. The fermentation product was primarily identified as rhamnolipid, which has high ability to reduce the surface tension when the concentration of critical micelle was 0.56 g·L-1, the surface tension of the aqueous solution can be reduced from 76.8 mN·m-1 to 38.5 mN·m-1.The removal efficiency of thorium from contaminated soil was studied using the fermented product of BS-1 as leaching lotion by elution and column leaching method. The results showed that the maximum removal efficiency can reach 70.14% under the condition of pH was 10, concentration of 3 g·L-1, the oscillating time of 72 h. When leaching amount was achieved 800 mL, the accumulation removal amount of thorium up to 65.03 mg·kg-1 using fermented product of BS-1 as eluting agent and superior to deionized water with column leaching experiment. Contrased the change of fractions of thorium form proportion in the contaminated soil before and after leaching, the residue form increased 10.16% and the oxidation combination form decreased 11.41%. Contrasted the change of fractions of thorium content in the contaminated soil before and after leaching, acid extractable form, oxidation combined form, organic binding state were reduced 83.25%, 75.22%, 60.70%， respectively. The stability of thorium was improved after leaching.
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  • [1] LI B, WANG N, WAN J, et al.In-situ gamma-ray survey of rare-earth tailings dams：A case study in Baotou and Bayan Obo Districts, China[J].Journal of Environmental Radioactivity,2016,151(1):304-310 10.1016/j.jenvrad.2015.10.027
    [2] 徐光宪, 师昌绪. 关于保护白云鄂博矿钍和稀土资源 避免黄河和包头受放射性污染的紧急呼吁[J]. 中国科学院院刊,2005,20(6):448-450
    [3] YAO Z, LI J, XIE H, et al.Review on remediation technologies of soil contaminated by heavy metals[J].Procedia Environmental Sciences,2012,16(4):722-729 10.1016/j.proenv.2012.10.099
    [4] MAO X, JIANG R, XIAO W, et al.Use of surfactants for the remediation of contaminated soils: A review[J].Journal of Hazardous Materials,2015,285:419-435 10.1016/j.jhazmat.2014.12.009
    [5] GADELLE F, WAN J, TOKUNAGA T K.Removal of uranium(VI) from contaminated sediments by surfactants[J].Journal of Environmental Quality,2001,30(2):470-478 10.2134/jeq2001.302470x
    [6] MULLIGAN C N.Environmental applications for biosurfactants[J].Environmental Pollution,2005,133(2):183-198 10.1016/j.envpol.2004.06.009
    [7] 张丽霞, 张虹, 卞立红, 等. 产表面活性剂菌株(QS-M2)的筛选及其特性研究[J]. 哈尔滨理工大学学报,2016,21(4):65-69 10.15938/j.jhust.2016.04.012
    [8] 王盼盼. 鼠李糖脂的发酵生产以及去除污染土壤中铅和镉的研究[D]: 无锡：江南大学,2016
    [9] 苏佳, 周丹, 李真真, 等. 鼠李糖脂对镧的淋洗效果及影响因素研究[J]. 中国稀土学报,2016,34(2):235-243 10.11785/S1000-4343.20160215
    [10] WEISBURG WG, BARNS SM, PELLETIER DA, et al. 16S ribosomal DNA amplification for phylogenetic study[J].Journal of Bacteriology,1991,173(2):697-703 10.1128/jb.173.2.697-703.1991
    [11] 包木太, 张金秋, 张娟, 等. 产糖脂类生物表面活性剂菌株鉴定及发酵条件优化[J]. 环境工程学报,2013,7(1):365-370
    [12] ZHANG X, XU D, YANG G, et al.Isolation and characterization of rhamnolipid producing Pseudomonas aeruginosa strains from waste edible oils[J].African Journal of Microbiology Research,2012,6(7):1466-1471 10.5897/AJMR11.1327
    [13] SEGHAL K G, ANTO T T, SELVIN J, et al.Optimization and characterization of a new lipopeptide biosurfactant produced by marine Brevibacterium aureum MSA13 in solid state culture[J].Bioresource Technology,2010,101(7):2389-2396 10.1016/j.biortech.2009.11.023
    [14] HAN F X, BANIN A, KINGERY W L, et al.New approach to studies of heavy metal redistribution in soil[J].Advances in Environmental Research,2003,8(1):113-120 10.1016/S1093-0191(02)00142-9
    [15] RAYRET G, LOPEZSANCHEZ J F, SAHUQUILLO A, et al.Improvement of the BCR three step sequential extraction procedure prior to the certification of new sediment and soil reference materials[J].Journal of Environmental Monitoring,1999,1(1):57-61 10.1039/a807854h
    [16] 李远功. 环境土壤样品中微量钍的测定[J]. 铀矿冶,2010,29(3):164-166 10.3969/j.issn.1000-8063.2010.03.011
    [17] 冯会粉. 生物表面活性剂产生菌发酵条件及产物特性研究[D]: 大连：大连工业大学,2016
    [18] 李政, 高艺文, 顾贵洲, 等. 一株生物表面活性剂产生菌的鉴定及发酵条件优化[J]. 环境工程学报,2016,10(5):2714-2718 10.12030/j.cjee.201412132
    [19] 崔中利, 刘卫东, 齐耀程, 等. 生物表面活性剂产生菌的分离培养及其产物特性研究[J]. 土壤,2004,36(6):644-647 10.3321/j.issn:0253-9829.2004.06.011
    [20] 罗娜. 铜绿假单胞菌产鼠李糖脂的能力及其对烃类污染物降解的研究[D]: 西安：西北大学,2016
    [21] OLIVEIRA F J S, VAZQUEZ L, CAMPOS N P D, et al.Production of rhamnolipids by a Pseudomonas alcaligenes strain[J].Process Biochemistry,2009,44(4):383-389 10.1016/j.procbio.2008.11.014
    [22] TH G N, NUNEZ A, FETT W, et al.Production of rhamnolipids by Pseudomonas chlororaphis, a Nonpathogenic Bacterium[J].Applied & Environmental Microbiology,2005,71(5):2288-2293 10.1128/AEM.71.5.2288-2293.2005
    [23] CHEN W C, JUANG R S, WEI Y H.Applications of a lipopeptide biosurfactant, surfactin, produced by microorganisms[J].Biochemical Engineering Journal,2015,103:158-169 10.1016/j.bej.2015.07.009
    [24] 李尤, 廖晓勇, 阎秀兰, 等. 鼠李糖脂淋洗修复重金属污染土壤的工艺条件优化研究[J]. 农业环境科学学报,2015,34(7):1287-1292 10.11654/jaes.2015.07.009
    [25] HONG K J, TOKUNAGA S, KAJIUCHI T.Evaluation of remediation process with plant-derived biosurfactant for recovery of heavy metals from contaminated soils[J].Chemosphere,2002,49(4):379-387 10.1016/S0045-6535(02)00321-1
    [26] HONG K J, TOKUNAGA S, ISHIGAMI Y, et al.Extraction of heavy metals from MSW incinerator fly ash using saponins[J].Chemosphere,2000,41(3):345-352 10.1016/S0045-6535(99)00489-0
    [27] CATHERINE N M, RAYMOND N Y, BEMARD F G, et al.metal removal from contaminated soil and sediments by the biosurfactant surfactin[J].Environmental Science & Technology,1999,33(21):3812-3820 10.1021/es9813055
    [28] 李光德, 张中文, 敬佩, 等. 茶皂素对潮土重金属污染的淋洗修复作用[J]. 农业工程学报,2009,25(10):231-235 10.3969/j.issn.1002-6819.2009.10.042
    [29] 雷鸣, 廖柏寒, 秦普丰. 土壤重金属化学形态的生物可利用性评价[J]. 生态环境学报,2007,16(5):1551-1556 10.3969/j.issn.1674-5906.2007.05.043
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  • 刊出日期:  2018-04-22
王维大, 张达, 韩剑宏, 姜庆宏, 李玉梅, 孙鹏, 张连科. Pseudomonas sp. BS-1发酵产物对土壤中钍的淋洗作用[J]. 环境工程学报, 2018, 12(4): 1198-1207. doi: 10.12030/j.cjee.201712036
引用本文: 王维大, 张达, 韩剑宏, 姜庆宏, 李玉梅, 孙鹏, 张连科. Pseudomonas sp. BS-1发酵产物对土壤中钍的淋洗作用[J]. 环境工程学报, 2018, 12(4): 1198-1207. doi: 10.12030/j.cjee.201712036
shu
WANG Weida, ZHANG Da, HAN Jianhong, JANG Qinghong, LI Yumei, SUN Peng, ZHANG Lianke. Leaching properties of thorium in soil by fermentation products of Pseudomonas sp. BS-1[J]. Chinese Journal of Environmental Engineering, 2018, 12(4): 1198-1207. doi: 10.12030/j.cjee.201712036
Citation: WANG Weida, ZHANG Da, HAN Jianhong, JANG Qinghong, LI Yumei, SUN Peng, ZHANG Lianke. Leaching properties of thorium in soil by fermentation products of Pseudomonas sp. BS-1[J]. Chinese Journal of Environmental Engineering, 2018, 12(4): 1198-1207. doi: 10.12030/j.cjee.201712036
shu

Pseudomonas sp. BS-1发酵产物对土壤中钍的淋洗作用

  • 1. 内蒙古科技大学能源与环境学院，包头 014010
  • 2. 华中科技大学环境与市政工程学院，武汉 430074
基金项目:

内蒙古自然科学基金资助项目（2016BS0511）

内蒙古科技大学创新基金（2016QDL-B08）

摘要: 从焦化废水污泥中筛选出一株产生物表面活性剂的菌株，将其命名为BS-1，16S rDNA系统发育学分析确定其属于假单胞菌属（Pesudomonas sp.）。该菌株发酵产物初步鉴定为鼠李糖脂，在其临界胶束浓度为0.56 g·L-1时，可将水溶液的表面张力从76.8 mN·m-1降至38.5 mN·m-1。将菌株发酵产物溶液作为淋洗剂，采用浸提和土柱淋洗的方法研究了BS-1发酵产物对污染土壤中钍的去除效果，结果表明：当pH为10，浓度为3 g·L-1，振荡时间为72 h时，钍的去除率可达70.14%；在柱状动态实验中，当淋洗量达到800 mL时，发酵产物溶液对钍的累积去除量最大为65.03 mg·kg-1，发酵产物溶液对钍的累积去除量明显优于去离子水。对比淋洗后土壤中钍的形态占比可知，残渣态增加10.16%，氧化结合态减少了11.41%。对比淋洗前后土壤中钍的形态含量可知，酸可提取态、氧化结合态、有机结合态分别减少了83.25%、75.22%、60.70%。淋洗后钍的稳定性增强。

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