高级搜索

某典型铬盐厂污染场地Cr(VI)还原菌的筛选、鉴定及还原特性

,  ,  ,  , 

downloadPDF

上一篇

下一篇

范琴, 王海燕, 颜湘华, 刘咏, 王兴润. 某典型铬盐厂污染场地Cr(VI)还原菌的筛选、鉴定及还原特性[J]. 环境工程学报, 2018, 12(3): 863-875. doi: 10.12030/j.cjee.201709216
引用本文: 范琴, 王海燕, 颜湘华, 刘咏, 王兴润. 某典型铬盐厂污染场地Cr(VI)还原菌的筛选、鉴定及还原特性[J]. 环境工程学报, 2018, 12(3): 863-875. doi: 10.12030/j.cjee.201709216
shu
FAN Qin, WANG Haiyan, YAN Xianghua, LIU Yong, WANG Xingrun. Screening, identification and reduction characteristics of Cr(VI)-reducing bacteria from a typical site contaminated by chromium plant[J]. Chinese Journal of Environmental Engineering, 2018, 12(3): 863-875. doi: 10.12030/j.cjee.201709216
Citation: FAN Qin, WANG Haiyan, YAN Xianghua, LIU Yong, WANG Xingrun. Screening, identification and reduction characteristics of Cr(VI)-reducing bacteria from a typical site contaminated by chromium plant[J]. Chinese Journal of Environmental Engineering, 2018, 12(3): 863-875. doi: 10.12030/j.cjee.201709216
shu

某典型铬盐厂污染场地Cr(VI)还原菌的筛选、鉴定及还原特性

  • 1.

    四川师范大学化学与材料科学学院，成都 610066

  • 2.

    污染场地安全修复技术国家工程实验室，北京 100012

  • 3.

    中国环境科学研究院环境基准与风险评估国家重点实验室，北京 100012

  • 4.

    清华大学固体废物处理与环境安全教育部重点实验室，北京 100084

  • 基金项目:

    国家环境保护公益性行业科研专项（201509031）

    污染场地安全修复技术国家工程实验室开放基金项目（NEL-SRT201705）

Screening, identification and reduction characteristics of Cr(VI)-reducing bacteria from a typical site contaminated by chromium plant

  • 1.

    College of Chemistry and Material Science, Sichuan Nomal University, Chengdu 610066, China

  • 2.

    National Engineering Laboratory for Site Remediation Technologies, Beijing 100012, China

  • 3.

    State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China

  • 4.

    China Key Laboratory for Solid Waste Management and Environment Safety, Ministry of Education, Tsinghua University, Beijing 100084, China

  • Fund Project:

  • 摘要: 从河南省义马市某典型铬盐厂污染场地3个典型区域获得污染土壤，以K2Cr2O7为供试物，用固体平板法筛选出8株菌，并进行Cr（VI）还原菌耐受性实验，挑选出耐受性较高的2株菌作为Cr（VI）的优势还原菌株，对这2株菌进行生长曲线的测定，用16S rRNA基因测序技术对其进行菌种鉴定，并研究了其在不同初始浓度、接种量、pH、温度条件下的还原特性，获得最佳还原条件。结果表明：YM4和YM7等2株菌对Cr（VI）具有高耐受性，其对50 mg·L-1和75 mg·L-1 Cr（VI）浓度的还原率均能达到97%以上。但Cr（VI）浓度提高到100 mg·L-1，YM4和YM7对Cr（VI）的还原率下降到了60%，说明100 mg·L-1 Cr（VI）对YM4和YM7产生了抑制作用。基因测序鉴定出YM4为芽孢杆菌，YM7为微小杆菌。在20～75 mg·L-1 范围内YM4和YM7均对Cr（VI）有较好去除效果。YM4的最佳还原条件是接种量为5%、pH为7.0～8.0、温度为35 ℃，YM7的最佳还原条件是接种量为5%、pH为7.0、温度为40 ℃。基于2株菌对Cr（VI）还原特性的研究，YM4和YM7可以用来作为微生物修复铬污染土壤的备选菌种。
    Abstract: Chromium contaminated soil was collected from three typical site contaminated by chromate plants in Henan, and strains were screened using solid plate method in which potassium dichromate was added as test material. Eight Cr（VI） reducing bacteria stains were screened out and resistance experiments were conducted. Two strains of higher tolerance were picked up and their growth curves were determined. 16S rRNA gene sequencing technology was adopted for primary identification, then their reduction characteristics were studied with different initial concentrations, inoculation amounts, pH, and temperature, and then it further obtained their optimal reduction conditions. Results showed that YM4 and YM7 were advantage strains for Cr（VI） reduction. The reduction rate of YM4 and YM7 for Cr（VI） with concentration of 50 mg·L-1 and 75 mg·L-1 were above 97%, but the reduction rate was decreased to 60% as its concentration increased to 100 mg·L-1, Therefore Cr（VI） with concentration of 100 mg·L-1 was inhibited to YM4 and YM7. As revealed by the gene sequencing, YM4 was identified as Bacillus and YM7 was identified Exiguobacterium. YM4 and YM7 all had great reduction effect for Cr（VI） with concentration of 20 to 75 mg·L-1. The optimal conditions for the reduction of Cr（VI） by strain YM4 were inoculation amount of 5%, initial pH of 7.0 to 8.0, and temperature of 35 ℃. The optimal conditions for the reduction of Cr（VI） by strain YM7 were inoculation amount of 5%, initial pH of 7.0, and temperature of 40 ℃. Based on the research of reduction characteristics of above two strains,YM4 and YM7 can be used as alternative bacteria for microbial remediation chromium contaminated soil.
  • 加载中
  • [1] 王兴润,张艳霞,王琪,等.铬污染建筑废物不同清洗剂的作用效果比较[J]. 化工学报,2012,63(10): 3255-3261 10.3969/j.issn.0438-1157.2012.10.035
    [2] 王兴润,颜湘华,赵涛,等.铬盐行业不同工艺废渣的产生特性和污染特性比较[J].环境工程,2015,33(S1):740-744
    [3] 黄顺红.铬渣堆场铬污染特征及其铬污染土壤微生物修复研究[D].长沙:中南大学,2009
    [4] 王兵.铬渣堆场重污染土壤微生物修复及Cr(VI)污染阻隔研究[D].长沙:中南大学,2010
    [5] 许友泽.铬渣堆场污染土壤微生物修复工艺研究[D].长沙:中南大学,2009
    [6] 王兴润,刘雪,颜湘华,等.铬渣污染土壤清洗剂筛选研究[J].环境科学研究,2010,23(11):1405-1409
    [7] 苏长青.铬污染土壤中Cr(VI)的微生物还原及Cr(III)的稳定性研究[D].长沙:中南大学,2010 10.7666/d.y1718920
    [8] 赵光辉,常文越,李雄勇,等.铬(VI)土著还原菌筛选及初步鉴定实验研究[J].环境保护科学,2013,39(3): 55-58
    [9] CHARDIN B, GIUDICI-ORTICONI M T, DE L G, et al.Hydrogenases in sulfate-reducing bacteria function as chromium reductase[J].Applied Microbiology and Biotechnology,2003,63(3): 315-321 10.1007/s00253-003-1390-8
    [10] BAE W, TAEGU K, JAEHAN J,et al.Purification and characterization of NADPH-dependent Cr(VI) reductase from Escherichia Coli ATCC 33456[J].Microbiology and Biotechnology,2000,10(5):580-586
    [11] AHMED Z, ABDUL R.Isolation of Cr(VI) reducing bacteria from industrial effluents and their potential use in bioremediation of chromium containing wastewater[J].Journal of Environment Sciences,2009,21(6):814-820 10.1016/S1001-0742(08)62346-3
    [12] ACKERLEY D F, GONZALEZ C F, PARK C H, et al.Chromate-reducing properties of soluble flavoproteins from Pseudomonas putida and Escherichia coli[J].Applied and Environmental Microbiology,2004,70(2):873-882 10.1128/AEM.70.2.873-882.2004
    [13] GUPTA K, CHATTERJEE C, GUPTA B.Isolation and characterization of heavy metal tolerant gram-positive bacteria with bioremedial properties from municipal waste rich soil of kestopur canal (Kolkata), West Bengal, India[J].Biologia, 2012,67(5):827-836 10.2478/s11756-012-0099-5
    [14] OKEKE B C.Bioremoval of hexavalent chromium from water by a salt tolerant bacterium, Exiguobacterium sp.GS1[J].Journal of Industrial Microbiology and Biotechnology,2008,35(12):1571-1579 10.1007/s10295-008-0399-5
    [15] 徐天生,欧杰,马晨晨,等.废弃钢厂耐Cr6+霉菌的分离鉴定及其生物学特性[J].微生物学杂志,2016,32(2):56-61
    [16] 李军,杨卓,纪宏伟,等.污染土壤中重金属Cr吸附菌株的筛选与鉴定[J].湖北农业科学,2016,55(9):2218-2221
    [17] 张剑.高效降Cr(VI)菌的筛选、鉴定及其降解特性[D].长沙:湘潭大学,2011
    [18] 贾燕,周思敏,董兰岚,等.一株除铬(VI)菌株的筛选和鉴定[J].现代预防医学,2016,43(11):2027-2030
    [19] 洪霞,张馨荃,严君华,等.Pseudomonas S2-3 菌株对Cr(VI)的耐受性及去除[J].环境工程学报,2016,10(3):1539-1545
    [20] 郑家传,张建荣,刘希雯,等.污染场地六价铬的还原和微生物稳定化研究[J].环境科学,2014,35(10):3882-3887
    [21] 国家环境保护总局.水质 六价铬的测定 二苯碳酰二肼分光光度法：GB 7467-1987[S].北京:中国标准出版社,1987
    [22] 冯杨阳,陈俊,刘波,等.一株对苯二甲酸降解菌的鉴定及其降解特性[J].化工学报,2006,57(8):1968-1973
    [23] DEY S, PAUL A K.Optimization of cultural conditions for growth associated chromate reduction by Arthrobacter sp.SUK 1201 isolated from chromite mine overburden[J].Journal of Hazardous Materials,2012,213-214(7):200-206 10.1016/j.jhazmat.2012.01.078
    [24] SAYEL H, BAHAFID W, JOUTEY N T, et al.Cr(VI) reduction by Enterococcus Gallinarum isolated from tannery waste-contaminated soil[J].Annals of Microbiology,2012,62(3):1269-1277 10.1007/s13213-011-0372-9
    [25] 马锦民.硫酸盐还原菌处理含铬(VI)废水的基础研究[D].上海:华东师范大学,2005
    [26] 张会敏,冯友军.一株野生细菌的16 SrDNA序列分析与系统发育树的构建[J].生物信息学,2005,3(1):1-4
    [27] 肖伟,王磊,李倬锴,等.六价铬还原细菌Bacillus Cereus S5.4还原机理及酶学性质研究[J].环境科学,2008,29(3):751-755
    [28] RAHMAN Z, SINGH V P.Cr(VI) reduction by Enterobacter sp.DU17 isolated from the tannery waste dump site and characterization of the bacterium and the Cr(VI) reductase[J].International Biodeterioration & Biodegradation,2014,91(11):97-103 10.1016/j.ibiod.2014.03.015
    [29] MCLEAN J, BEVERIDGE T J.Chromate reduction by a pseudomonad isolated from a site contaminated with chromated copper arsenate[J].Applied and Environmental Microbiology,2001,67(3):1076-1084 10.1128/AEM.67.3.1076-1084.2001
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  2053
  • HTML全文浏览数:  1679
  • PDF下载数:  485
  • 施引文献:  0
出版历程
  • 刊出日期:  2018-03-22

某典型铬盐厂污染场地Cr(VI)还原菌的筛选、鉴定及还原特性

  • 1. 四川师范大学化学与材料科学学院，成都 610066
  • 2. 污染场地安全修复技术国家工程实验室，北京 100012
  • 3. 中国环境科学研究院环境基准与风险评估国家重点实验室，北京 100012
  • 4. 清华大学固体废物处理与环境安全教育部重点实验室，北京 100084
基金项目:

国家环境保护公益性行业科研专项（201509031）

污染场地安全修复技术国家工程实验室开放基金项目（NEL-SRT201705）

摘要: 从河南省义马市某典型铬盐厂污染场地3个典型区域获得污染土壤，以K2Cr2O7为供试物，用固体平板法筛选出8株菌，并进行Cr（VI）还原菌耐受性实验，挑选出耐受性较高的2株菌作为Cr（VI）的优势还原菌株，对这2株菌进行生长曲线的测定，用16S rRNA基因测序技术对其进行菌种鉴定，并研究了其在不同初始浓度、接种量、pH、温度条件下的还原特性，获得最佳还原条件。结果表明：YM4和YM7等2株菌对Cr（VI）具有高耐受性，其对50 mg·L-1和75 mg·L-1 Cr（VI）浓度的还原率均能达到97%以上。但Cr（VI）浓度提高到100 mg·L-1，YM4和YM7对Cr（VI）的还原率下降到了60%，说明100 mg·L-1 Cr（VI）对YM4和YM7产生了抑制作用。基因测序鉴定出YM4为芽孢杆菌，YM7为微小杆菌。在20～75 mg·L-1 范围内YM4和YM7均对Cr（VI）有较好去除效果。YM4的最佳还原条件是接种量为5%、pH为7.0～8.0、温度为35 ℃，YM7的最佳还原条件是接种量为5%、pH为7.0、温度为40 ℃。基于2株菌对Cr（VI）还原特性的研究，YM4和YM7可以用来作为微生物修复铬污染土壤的备选菌种。

English Abstract

参考文献 (29)

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心