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曲静怡, 杨统一, 陈芳艳, 唐玉斌. 古运河(镇江段)沉积物酶活性及其与氮磷阴离子的相关性[J]. 环境工程学报, 2014, 8(3): 1057-1061.
引用本文: 曲静怡, 杨统一, 陈芳艳, 唐玉斌. 古运河(镇江段)沉积物酶活性及其与氮磷阴离子的相关性[J]. 环境工程学报, 2014, 8(3): 1057-1061.
shu
Qu Jingyi, Yang Tongyi, Chen Fangyan, Tang Yubin. Correlation between enzyme activity and anion concentration of nitrogen and phosphorus in sediments of Zhenjiang reach of the ancient canal,China[J]. Chinese Journal of Environmental Engineering, 2014, 8(3): 1057-1061.
Citation: Qu Jingyi, Yang Tongyi, Chen Fangyan, Tang Yubin. Correlation between enzyme activity and anion concentration of nitrogen and phosphorus in sediments of Zhenjiang reach of the ancient canal,China[J]. Chinese Journal of Environmental Engineering, 2014, 8(3): 1057-1061.
shu

古运河(镇江段)沉积物酶活性及其与氮磷阴离子的相关性

  • 1.

    江苏科技大学生物与化学工程学院, 镇江 212018

  • 基金项目:

    江苏科技大学博士科研启动基金项目（35211201）

  • 中图分类号: X522

Correlation between enzyme activity and anion concentration of nitrogen and phosphorus in sediments of Zhenjiang reach of the ancient canal,China

  • 1.

    School of Biological and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, China

  • Fund Project:

  • 摘要: 以古运河镇江段为研究对象，研究了表层沉积物中碱性磷酸酶活性、硝酸还原酶活性和亚硝酸还原酶活性的时空变化以及3种酶活性与河流表层沉积物中氮磷阴离子浓度及上覆水水质的相关性。结果表明，镇江古运河表层沉积物中碱性磷酸酶活性、硝酸还原酶活性和亚硝酸还原酶活性具有显著的时间和区域性差异，枯水期（12月）各酶活性较高，分别介于0.29~0.32 mg/（g·24 h）、0.13~0.19 mg/（g·24 h）、3.05~5.21 mg/（g·24 h）；丰水期 （5月） 各酶活性较低，变化范围分别为0.18~0.29 mg/（g·24 h）、0.08~0.14 mg/（g·24 h）、2.95~3.64 mg/（g·24 h）。冗余分析结果显示，古运河表层沉积物酶活性在枯水期差异较大，丰水期差异较小，各酶活性与其相关离子浓度呈显著正相关（P<0.05），硝酸还原酶和亚硝酸还原酶与上覆水的水质呈正相关。3种酶活性可被作为判断古运河镇江段沉积物富营养化负荷的参考性指标，硝酸还原酶和亚硝酸还原酶还可作为评价古运河镇江段水质的参考性指标。
    Abstract: Zhenjiang reach of the ancient canal of China was regarded as research object. The temporal and spatial variation of activity of alkaline phosphatase,nitrate reductase and nitrite reductase in surface layer of the sediments were studied. The correlation between the three enzyme activities and the concentration of nitrogen and phosphorus in surface layer of the sediments and overlying water quality were also studied. The results showed that there exist temporal and regional variations in the three enzyme activities. The enzyme activities were higher in the dry season (December),with values of 0.29 mg/(g·24 h) to 0.32 mg/(g·24 h),0.13 mg/(g·24 h) to 0.19 mg/(g·24 h),3.05 mg/(g·24 h) to 5.21 mg/(g·24 h),respectively. In contrast,lower enzyme activities were detected in the flood season (May),with values of 0.18 mg/(g·24 h) to 0.29 mg/(g·24 h),0.08 mg/(g·24 h) to 0.14 mg/(g·24 h),2.95 mg/(g·24 h) to 3.64 mg/(g·24 h),respectively. Redundancy analysis showed that the difference of the enzyme activities is larger in dry season than that in flood season. The activities were positively correlated to the relevant anion concentrations (P<0.05). Moreover,the nitrate reductase and nitrite reductase activities were positively correlated with overlying water quality. The three enzyme activities could be regarded as reference index for judging eutrophication load of sediments in Zhenjiang reach of the ancient canal of China. Nitrate reductase and nitrite reductase activities could also be regarded as reference index for evaluating the water quality of Zhenjiang reach of the ancient canal.
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  • [1] Chen Jianjun, Lu Shaoyong, Zhao Yikun,et al. Effects of overlying water aeration on phosphorus fractions and alkaline phosphatase activity in surface sediment. Journal of Environmental Sciences,2011,23(2):206-211
    [2] Wang Shengrui, Jiao Lixin, Yang Suwen,et al. Effects of organic matter and submerged macrophytes on variations of alkaline phosphatase activity and phosphorus fractions in lake sediment. Journal of Environmental Management,2012,113(12):355-360
    [3] 龚钢明,吕玉涛,管世敏,等. 乳酸菌亚硝酸盐还原酶制备及酶学性质. 中国酿造,2011,(1):58-60 Gong Gangming,Lü Yutao,Guan Shimin,et al. Preparation and properties of nitrite reductase from Lactobacillus.China Brewing, 2011,(1):58-60(in Chinese)
    [4] 宋厚燃,马利民,闵真真. 梁滩河沉积物中氮磷垂直分布研究. 环境污染与防治,2012,34(6):42-50 Song Houran, Ma Limin,Min Zhenzhen. Study on the vertical distribution of nitrogen and phosphorus in the sediments of Liangtanhe River. Environmental Pollution & Control,2012,34(6):42-50(in Chinese)
    [5] Hülya Arslan,Gürcan Güleryüz. A study on nitrate reductase activity (NRA) of geophytes from Mediterranean environment. Flora,2005,200(5):434-443
    [6] Fungyi Chow,Mariana C. de Oliveira,et al. In vitro assay and light regulation of nitrate reductase in red alga Gracilaria chilensis.Journal of Plant Physiology,2004,161(7):769-776
    [7] 白红英,韩建刚,张一平. 不同土层土壤理化性状与反硝化酶活性,N2O排放通量的相关性研究.农业环境保护,2002,21(3):193-196 Bai Hongying,Han Jiangang,Zhang Yiping. Correlations between physical,chemical and biological properties and denitrifying enzyme activity,N2O flux in soil profiles. Agro-Environmental Protection,2002,21(3):193-196(in Chinese)
    [8] 杜婵娟,白先放,谢庆武. 亚硝酸还原酶产生菌的筛选及酶学性质的研究. 广西轻工业,2011,27(6):7-8 Du Chanjuan, Bai Xianfang, Xie Qiuwu. Study on nitrite reductase producing strains and enzyme properties. Guangxi Journal of Light Industry, 2011,27(6):7-8(in Chinese)
    [9] 徐龙,梁蕊芳,靳烨. 肉制品中亚硝酸还原酶的应用进展. 食品工业科技,2012,33(3):413-416 Xu Long,Liang Ruifang,Jin Ye. Research progress in nitrite reductase in meat products.Science and Technology of Food Industry,2012,33(3):413-416(in Chinese)
    [10] Li Yin,Chrõst R. J. Microbial enzymatic activities in aerobic activated sludge model reactors. Enzyme Microb. Tech., 2006,39(4):568-572
    [11] Boczar B. A.,Forney L. J.,Begley W. M.,et al. Characterization and distribution of esterase activity in activated sludge. Water Research,2001,35(17):4208-4216
    [12] Whiteley C. G.,Burgess J. E.,Melamane X.,et al. The enzymology of sludge solubilisation utilizing sulphate-reducing system:The properties of lipases.Water Research,2003,37(2):289-296
    [13] 李茵,罗翠,Chróst R. J. 城市污水生物处理系统中微生物酶的活性及其分布.环境污染与防治,2007,29(5):333-335 Li Yin,Luo Cui,Chróst R J. Microbial enzymatic activities in a municipal wastewater biotreatment system. Environmental Pollution & Control,2007,29(5):333-335(in Chinese)
    [14] 夏卓英,陈芳,宋春雷,等. 长江中下游部分湖泊沉积物碱性磷酸酶分布及其作用研究. 水生生物学报,2007,31(1):9-17 Xia Zhuoying,Chen Fang,Song Chunlei. et al. A study on distribution and roles of alkaline phosphatase in sediments of some lakes in the middle and lower reaches of the Yangtze river. Acta Hydrobiologica Sinica,2007,31(1):9-17(in Chinese)
    [15] 刘芷宇. 根际研究法(第1版). 南京:江苏科学技术出版社,1997
    [16] 国家环境保护总局. 水和废水监测分析方法(第4版).北京:中国环境科学出版社,2008
    [17] 章婷曦,王晓蓉,金相灿. 太湖沉积物中碱性磷酸酶活力(APA)和磷形态的垂向特征及相关性. 农业环境科学学报,2007,26(1):36-40 Zhang Tingxi,Wang Xiaorong,Jin Xiangcan. Vertical variation of alkaline phosphatase activity and phosphorus forms in the taihu lake sediment and the relationship between them. Journal of Agro-Environment Science,2007,26(1):36-40(in Chinese)
    [18] 薛雄志,洪华生.香港维多利亚港沉积物中ALPase活力与各形态磷及微生物特性的关系. 海洋学报,1997,19(5):133-137 Xue Xiongzhi, Hong Huasheng. The relationship among the activity of ALPase, the various forms of phosphorus and microbial characteristics in the Victoria Harbour sediments of Hongkong. Acta Oceanologica Sinica, 1997, 19 (5):133-137(in Chinese)
    [19] 薛雄志,洪华生. 厦门西海域沉积物中碱性磷酸酶活力分布、动态及其与各形态磷的关系.海洋学报,1995,17(5):81-87 Xue Xiongzhi, Hong Huasheng. The relationship between alkaline phosphatase activity and distribution and various forms of phosphorus in the sediments of Xiamen west area. Acta Oceanologica Sinica, 1995, 17(5):81-87(in Chinese)
    [20] 李小平,方涛,敖鸿毅,等.东湖沉积物中dNaR活性和硝酸盐还原菌的垂向分布.中国环境科学,2010,30 (2):228-232 Li Xiaoping,Fang Tao,Ao Hongyi,et al. Vertical variation of dissimilatory nitrate reductase activity and nitrate reducer in the sediments of Lake Donghu.China Environmental Science,2010,30(2):228-232(in Chinese)
    [21] Kcith S. M.,Macfarlane G. T.,Herbert R. A. Dissimilatory nitrate reduction by a strain of elostridium butyricum isolated from estuarine sediments. Archives of Microbiology,1982,132(1):62-66
    [22] 许苗苗,陈义伦,潘燕,等.菠菜体内硝酸还原酶部分酶学性质及贮藏控制.食品与发酵工业,2008,34(11):21-24 Xu Miaomiao,Chen Yilun,Pan Yan, et al. Some enzymatic properties and storage control of nitrate reductase of spinach. Food and Fermentation Industries,2008,34 (11):21-24(in Chinese)
    [23] 何玲,张祖德. 不同条件下辣椒发酵过程硝酸还原酶活性和亚硝酸盐含量变化.中国酿造,2012,31(1):125-129 He Ling,Zhang Zude. Changes of nitrate reductase activity and nitrite contents in pepper under different fermentation conditions.China Brewing,2012,31(1):125-129(in Chinese)
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  • 收稿日期:  2013-01-24
  • 刊出日期:  2014-03-01
曲静怡, 杨统一, 陈芳艳, 唐玉斌. 古运河(镇江段)沉积物酶活性及其与氮磷阴离子的相关性[J]. 环境工程学报, 2014, 8(3): 1057-1061.
引用本文: 曲静怡, 杨统一, 陈芳艳, 唐玉斌. 古运河(镇江段)沉积物酶活性及其与氮磷阴离子的相关性[J]. 环境工程学报, 2014, 8(3): 1057-1061.
shu
Qu Jingyi, Yang Tongyi, Chen Fangyan, Tang Yubin. Correlation between enzyme activity and anion concentration of nitrogen and phosphorus in sediments of Zhenjiang reach of the ancient canal,China[J]. Chinese Journal of Environmental Engineering, 2014, 8(3): 1057-1061.
Citation: Qu Jingyi, Yang Tongyi, Chen Fangyan, Tang Yubin. Correlation between enzyme activity and anion concentration of nitrogen and phosphorus in sediments of Zhenjiang reach of the ancient canal,China[J]. Chinese Journal of Environmental Engineering, 2014, 8(3): 1057-1061.
shu

古运河(镇江段)沉积物酶活性及其与氮磷阴离子的相关性

  • 1. 江苏科技大学生物与化学工程学院, 镇江 212018
  • 收稿日期:  2013-01-24
基金项目:

江苏科技大学博士科研启动基金项目（35211201）

摘要: 以古运河镇江段为研究对象，研究了表层沉积物中碱性磷酸酶活性、硝酸还原酶活性和亚硝酸还原酶活性的时空变化以及3种酶活性与河流表层沉积物中氮磷阴离子浓度及上覆水水质的相关性。结果表明，镇江古运河表层沉积物中碱性磷酸酶活性、硝酸还原酶活性和亚硝酸还原酶活性具有显著的时间和区域性差异，枯水期（12月）各酶活性较高，分别介于0.29~0.32 mg/（g·24 h）、0.13~0.19 mg/（g·24 h）、3.05~5.21 mg/（g·24 h）；丰水期 （5月） 各酶活性较低，变化范围分别为0.18~0.29 mg/（g·24 h）、0.08~0.14 mg/（g·24 h）、2.95~3.64 mg/（g·24 h）。冗余分析结果显示，古运河表层沉积物酶活性在枯水期差异较大，丰水期差异较小，各酶活性与其相关离子浓度呈显著正相关（P<0.05），硝酸还原酶和亚硝酸还原酶与上覆水的水质呈正相关。3种酶活性可被作为判断古运河镇江段沉积物富营养化负荷的参考性指标，硝酸还原酶和亚硝酸还原酶还可作为评价古运河镇江段水质的参考性指标。

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