常温下基质与时间对ANAMMOX污泥活性保藏影响

李祥, 朱莉, 黄勇, 袁怡, 张丽. 常温下基质与时间对ANAMMOX污泥活性保藏影响[J]. 环境工程学报, 2013, 7(9): 3333-3338.
引用本文: 李祥, 朱莉, 黄勇, 袁怡, 张丽. 常温下基质与时间对ANAMMOX污泥活性保藏影响[J]. 环境工程学报, 2013, 7(9): 3333-3338.
Li Xiang, Zhu Li, Huang Yong, Yuan Yi, Zhang Li. Effect of nutrition and time on ANAMMOX sludge activity preservation at room temperature[J]. Chinese Journal of Environmental Engineering, 2013, 7(9): 3333-3338.
Citation: Li Xiang, Zhu Li, Huang Yong, Yuan Yi, Zhang Li. Effect of nutrition and time on ANAMMOX sludge activity preservation at room temperature[J]. Chinese Journal of Environmental Engineering, 2013, 7(9): 3333-3338.

常温下基质与时间对ANAMMOX污泥活性保藏影响

  • 基金项目:

    国家自然科学基金资助项目(51008202)

    江苏省环境科学与工程重点实验室开放课题(ZD081202)

    江苏省环保厅重大项目(201104)

    苏州科技学院基金项目(XKY201103)

  • 中图分类号: X703.1

Effect of nutrition and time on ANAMMOX sludge activity preservation at room temperature

  • Fund Project:
  • 摘要: 采用厌氧氨氧化污泥,通过脱氮效能的测定,研究了常温(15±2)℃下保藏过程中基质添加与保藏时间对污泥活性的影响以及探讨长期保持污泥活性的保藏策略。结果表明,在无营养物添加的情况下,保藏的前45天厌氧氨氧化污泥氮去除速率由0.107 kg/(m3·d)下降到0.025 kg/(m3·d),其速率下降最快,但是经过6 d培养,污泥的活性可以得到恢复;当保藏365 d后,厌氧氨氧化污泥活性基本消失,即使通过11 d恢复也无明显的厌氧氨氧化特性。说明常温下保藏时间对厌氧氨氧化污泥活性影响显著,随着保藏时间的延长污泥氮去除速率会大幅降低,甚至消失。根据衰减指数模型拟合出常温状态下厌氧氨氧化污泥活性衰减模型为y=0.547×exp(-0.0109×t)。通过定期(15 d)投加营养物,经过150 d和365 d保藏后的厌氧氨氧化污泥仍然保持48%和31%的活性。说明定期投加营养物可以有效缓解厌氧氨氧化污泥活性在常温保藏过程中的衰减,适用于厌氧氨氧化污泥常温下长期保藏。
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  • [1] Jetten M. S. M., Cirpus I., Kartal B., et al. 1994-2004: 10 years of research on the anaerobic oxidation of ammonium. Biocheml. Socl. Tranl., 2005, 33(1): 119-123
    [2] 李祥,黄勇,郑宇慧,等.厌氧氨氧化富集培养研究及其工业应用进展.水处理技术, 2010,36(5):18-21 Li X., Huang Y., Zheng Y. H., et al. Anammox enrichment culture and its progress in industrial applications. Technology of Water Treatment, 2010, 36(5):18-21(in Chinese)
    [3] 唐崇俭,郑平,陈婷婷.厌氧氨氧化工艺的菌种、启动与效能.化工学报, 2010, 61 (10): 2510-2516 Tang C.J., Zheng P., Cheng T. T. Inoculum, start-up and performance of Anammox process. CIESC Journal, 2010, 61 (10): 2510-2516(in Chinese)
    [4] Qing N. S., Yu G. B., Cheng W. C., et al. Fast start-up, performance and microbial community in a pilot-scale anammox reactor seeded with exotic mature granules. Bioresource Technology, 2011, 102(3): 2448-2454
    [5] Nutchanat C., Suwanchai N. Anammox enrichment from different conventional sludges. Chemosphere, 2007, 66(11): 2225-2232
    [6] Wang T., Zhang H. M., Gao D.W., et al. Enrichment of Anammox bacteria in seed sludges from different wastewater treating processes and start-up of Anammox process. Desalination, 2011,271(1): 193-198
    [7] 洪义国,李猛,顾继东.海洋氮循环中细菌的厌氧氨氧化.微生物学报, 2009,49 (3):281-286 Hong G. Y., Li M., Gu J.D. Bacterial anaerobic ammonia oxidation (Anammox) in the marine nitrogen cycle:A review. Acta Microbiologica Sinica, 2009,49 (3):281-286(in Chinese)
    [8] 陈婷婷,郑平,胡宝兰.厌氧氨氧化菌的物种多样性与生态分布.应用生态学报,2009,20(5): 1229-1235 Chen T. T., Zheng P., Hu B. L. Species diversity and ecological distribution of anaerobic ammonium-oxidizing bacteria. Chinese Journal of Applied Ecology, 2009,20(5): 1229-1235 (in Chinese)
    [9] Strous M., Heijnen J. J., Kuenen J. G.,et al. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms. Appl. Microbiol. Biotechnol., 1998, 50(5): 4149-4163
    [10] van der S. W., Abma W. R., Blommers D., et al. Start up of reactors for anoxic ammonium oxidation: Experiences from the first full scale ANAMMOX reactor in Rotterda. Water Research, 2007, 41(18): 4149-4163
    [11] Zhang L., Yang J. C., Hira D.,et al. High-rate nitrogen removal from anaerobic digester liquor using an up-flow anammox reactor with polyethylene sponge as a biomass carrier. Journal of Bioscience and Bioengineering, 2011, 111(3): 306-311
    [12] Yang J. C., Zhang L., Daisuke H., et al. High-rate nitrogen removal by the Anammox process at ambient temperature. Bioresource Technology, 2011, 102(2):672-676
    [13] Samik B., Rima B., Tapas N. Start-up and stabilization of an Anammox process from a non-acclimatized sludge in CSTR. J. Ind. Microbiol. Biotechnol., 2010, 37(9):943-952
    [14] Helio L., Sebastia P., Ramon G., et al. Start-up and enrichment of a granular anammox SBR to treat high nitrogen load wastewaters. Journal of Chemical Technology and Biotechnology, 2008, 83(1): 233-241
    [15] 唐崇俭,郑平,陈建伟.中试厌氧氨氧化反应器的启动与调控.生物工程学报,2009,25(3):406-412 Tang C.J., Zheng P., Cheng J. W. Start-up and process control of a pilot-scale Anammox bioreactor at ambient temperature. Chinese Journal of Biotechnology, 2009, 25(3):406-412 (in Chinese)
    [16] Michael J., Rothrock J., Matias B., et al. Long-term preservation of anammox bacteria. Appl. Microbiol. Biotechnol., 2011, 92(1):147-157
    [17] Siegfried E. V., Joke G., Han V., et al. Reactivation of aerobic and anaerobic ammonium oxidizers in OLAND biomass after long-term storage. Appl. Microbiol. Biotechnol., 2007, 74(6):1376-1384
    [18] 李祥,郑宇慧,黄勇,等. 保存温度及时间对厌氧氨氧化污泥活性的影响. 中国环境科学,2011,31(1):56-61 Li X., Zheng Y. H., Huang Y.,et al. Effect of preservation temperature and time on ANAMMOX sludge activity. China Environmental Science, 2011,31(1):56-61(in Chinese)
    [19] 国家环境保护局.水和废水监测分析方法(第4版). 北京:中国环境科学出版社, 2002.258-282
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    [21] Dosta J., Fernandrz I., Vazquez-Padin J.R., et al. Short-and long-term effects of temperature on the Anammox process. Journal of Hazardous Materials, 2008, 154(1-3): 688-693
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出版历程
  • 收稿日期:  2012-07-27
  • 刊出日期:  2013-09-15

常温下基质与时间对ANAMMOX污泥活性保藏影响

  • 1.  苏州科技学院环境科学与工程学院, 苏州 215011
  • 2.  同济大学环境学院, 上海 200092
基金项目:

国家自然科学基金资助项目(51008202)

江苏省环境科学与工程重点实验室开放课题(ZD081202)

江苏省环保厅重大项目(201104)

苏州科技学院基金项目(XKY201103)

摘要: 采用厌氧氨氧化污泥,通过脱氮效能的测定,研究了常温(15±2)℃下保藏过程中基质添加与保藏时间对污泥活性的影响以及探讨长期保持污泥活性的保藏策略。结果表明,在无营养物添加的情况下,保藏的前45天厌氧氨氧化污泥氮去除速率由0.107 kg/(m3·d)下降到0.025 kg/(m3·d),其速率下降最快,但是经过6 d培养,污泥的活性可以得到恢复;当保藏365 d后,厌氧氨氧化污泥活性基本消失,即使通过11 d恢复也无明显的厌氧氨氧化特性。说明常温下保藏时间对厌氧氨氧化污泥活性影响显著,随着保藏时间的延长污泥氮去除速率会大幅降低,甚至消失。根据衰减指数模型拟合出常温状态下厌氧氨氧化污泥活性衰减模型为y=0.547×exp(-0.0109×t)。通过定期(15 d)投加营养物,经过150 d和365 d保藏后的厌氧氨氧化污泥仍然保持48%和31%的活性。说明定期投加营养物可以有效缓解厌氧氨氧化污泥活性在常温保藏过程中的衰减,适用于厌氧氨氧化污泥常温下长期保藏。

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