一株1，2-二氯苯降解菌的分离鉴定及其降解特性
Isolation and characterization of a 1，2-dichlorobenzene-degrading bacterial strain
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摘要: 采用富集驯化方法，从盐城芦苇湿地根际土壤中分离得到一株可高效降解1，2-二氯苯的菌株，命名为DL-1。该菌株可以在以1，2-二氯苯为惟一碳源的无机培养基上生长，能够耐受最高浓度为200 mg/L的1，2-二氯苯。根据形态特征观察、生理生化鉴定和16S rDNA序列同源性分析，该目标菌株被鉴定为蜡质芽孢杆菌(Bacillus cereus)。菌株DL-1对1，2-二氯苯降解性能研究表明，该菌株为一株兼性厌氧菌，其适宜降解浓度、适宜温度、适宜pH值和适宜接种量分别为120 mg/L、32℃、7和10%，在适宜降解条件下降解1，2-二氯苯4 d其降解率达到80.3%。本实验为利用该菌株降解1，2-二氯苯污水的应用提供了理论基础。Abstract: A bacterial strain named DL-1 was isolated from the reed wetland in Yancheng City by enriched domestication culture， which was able to grow on minimal medium supplemented with 1，2-dichlorobenzene as the sole source of carbon. The highest endurance capability of this strain to 1，2-dichlorobenzene was 200 mg/L. Based on the morphological characteristics observation， biochemical properties identification and 16S rDNA sequence homology analysis，strain DL-1 was identified as Bacillus cereus. The degradation characters of 1，2-dichlorobenzene indicated that the strain was a facultative anaerobe， the optimum degradation concentration， pH value， temperature and inoculation amount were 120 mg/L， pH 7， 30℃ and 10%. Under the appropriate degradation conditions the degradation rate of strain DL-1 reached 80.3% within four days. This experiment could provide a theoretical basis for the application of the strain degraded 1，2-dichlorobenzene sewage in future.
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