PVA降解混合菌系生长和产酶的营养条件分析
Aanalysis on the nutritional condition of growth and enzyme production for PVA degradation mixed strains
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摘要: 研究碳源、氮源以及不同金属离子对PVA降解混合菌系生长和产酶的影响。结果表明，混合菌系生长过程中无机氮源优于有机氮源；补充碳源可促进混合菌系的生长，但酶活比不添加时稍有降低。金属离子对酶活影响的正交实验表明Mg2+浓度影响最大，其次是Fe2+和K+浓度，Ca2+、Mn2+、Zn2+和Cu2+浓度影响较小；最佳浓度分别为Mg2+ 0.05 g/L、Fe2+ 0.04 g/L、K+ 2.0 g/L和Ca2+0.05 g/L。Abstract: The influence for growth and enzyme production of different concentration of carbon and nitrogen source and metal ions was investigated in a mixed strains. The results indicated that the inorganic nitrogen sources were better than organic nitrogen sources during the growth of mixed strains. And other carbon sources could promote the growth of microorganism but restrain the activity of PVA degrading enzyme. The orthogonal test results of metal ions on enzyme activity indicated that the concentration of Mg2+ was the most important influenceing factor and Fe2+and K+ were secondary factors. The influence of the concentrations of Ca2+，Mn2+，Zn2+ and Cu2+ was slight. The optimum concentration was Mg2+ 0.05 g/L，Fe2+ 0.04 g/L，K+ 2.0 g/L and Ca2+0.05 g/L， respectively.
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Key words:
- PVA /
- degrading enzyme /
- carbon source /
- nitrogen source /
- metal ions
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