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苯醚甲环唑和手性苯醚甲环唑醇对近头状尖孢藻的胁迫效应

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庄吉阳, 吴慧珍, 钱鸣蓉, 金哲雄, 盛颖霏, 章虎, 王军良, 孙娜波. 苯醚甲环唑和手性苯醚甲环唑醇对近头状尖孢藻的胁迫效应[J]. 生态毒理学报, 2024, 19(6): 335-343. doi: 10.7524/AJE.1673-5897.20240618001
引用本文: 庄吉阳, 吴慧珍, 钱鸣蓉, 金哲雄, 盛颖霏, 章虎, 王军良, 孙娜波. 苯醚甲环唑和手性苯醚甲环唑醇对近头状尖孢藻的胁迫效应[J]. 生态毒理学报, 2024, 19(6): 335-343. doi: 10.7524/AJE.1673-5897.20240618001
shu
ZHUANG Jiyang, WU Huizhen, QIAN Mingrong, JIN Zhexiong, SHENG Yingfei, ZHANG Hu, WANG Junliang, SUN Nabo. The Stress Effects of Difenoconazole and Chiral Difenoconazole-alcohol on Selenastrum capricornutum[J]. Asian journal of ecotoxicology, 2024, 19(6): 335-343. doi: 10.7524/AJE.1673-5897.20240618001
Citation: ZHUANG Jiyang, WU Huizhen, QIAN Mingrong, JIN Zhexiong, SHENG Yingfei, ZHANG Hu, WANG Junliang, SUN Nabo. The Stress Effects of Difenoconazole and Chiral Difenoconazole-alcohol on Selenastrum capricornutum[J]. Asian journal of ecotoxicology, 2024, 19(6): 335-343. doi: 10.7524/AJE.1673-5897.20240618001
shu

苯醚甲环唑和手性苯醚甲环唑醇对近头状尖孢藻的胁迫效应

  • 1. 浙江工业大学环境学院, 杭州 310032;

  • 2. 浙江树人学院生物与环境工程学院, 杭州 310015;

  • 3. 浙江树人学院交叉科学研究院, 杭州 310015;

  • 4. 绿城农科检测技术有限公司, 杭州 310052;

  • 5. 浙江工业大学绍兴研究院, 绍兴 312000

    • 作者简介: 庄吉阳(2000—)，男，硕士研究生，研究方向为环境毒理学，E-mail:1248448000@qq.com
    通讯作者: 王军良(1978—)，男，博士，教授，主要研究方向为超亚临界流体、高浓度有机废水处理与资源化和二氧化碳地质封存。E-mail:upupwang@zjut.edu.cn;  孙娜波(1975-),女,博士,教授,主要研究方向为有机合成。E-mail:sunnabo@zjsru.edu.cn
  • 基金项目:

    浙江省“三农九方”农业科技协作计划项目(2024SNJF042)

  • 中图分类号: X171.5

The Stress Effects of Difenoconazole and Chiral Difenoconazole-alcohol on Selenastrum capricornutum

  • 1. College of Environment, Zhejiang University of Technology, Hangzhou 310032, China;

  • 2. College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China;

  • 3. Interdisciplinary Research Academy, Zhejiang Shuren University, Hangzhou 310015, China;

  • 4. Greentown Agricultural Science Testing Technology Co. Ltd., Hangzhou 310052, China;

  • 5. Shaoxing Research Institute, Zhejiang University of Technology, Shaoxing 312000, China

    • Corresponding authors: WANG Junliang ;  SUN Nabo
  • Fund Project:

  • 摘要: 本研究以近头状尖孢藻为受试对象，通过测定半数有效浓度(EC50)、光合色素水平、丙二醛(MDA)含量及抗氧化酶活性，评估了苯醚甲环唑及其降解产物苯醚甲环唑醇对藻的生物毒性，重点分析了手性苯醚甲环唑醇对藻生物毒性的立体选择性差异。本文所测苯醚甲环唑和苯醚甲环唑醇96 h的EC50值分别为0.67 mg·L-1和1.74 mg·L-1，R-(+)-苯醚甲环唑醇和S-(-)-苯醚甲环唑醇96 h的EC50值分别为2.74 mg·L-1和1.68 mg·L-1。同时基于藻受苯醚甲环唑、苯醚甲环唑醇和苯醚甲环唑醇对映体胁迫后，氧化损伤相关参数的变化趋势以及光合作用载体叶绿素含量的程度变化趋势，得出苯醚甲环唑对近头状尖孢藻的毒性强于苯醚甲环唑醇，手性苯醚甲环唑醇对藻的胁迫表现为S-(-)-对映体＞消旋体＞R-(+)-对映体。
    Abstract: This study evaluated the biological toxicity of difenoconazole and its degradation product difenoconazole-alcohol to Selenastrum capricornutum by measuring median effective concentration (EC50), photosynthetic pigment levels, malondialdehyde (MDA) content, and antioxidant enzyme activity, with a focus on analyzing the stereoselective differences in chiral difenoconazole-alcohol’s biological toxicity to algae. The EC50 values of difenoconazole and difenoconazole-alcohol after 96 hours exposure were 0.67 mg·L-1 and 1.74 mg·L-1, respectively. The EC50 values of R-(+)- difenoconazole-alcohol and S-(-)-difenoconazole-alcohol after 96 hours exposure were 2.74 mg·L-1 and 1.68 mg·L-1, respectively. At the same time, based on the evaluation of changing the trend of oxidative damage-related parameters the levels of chlorophyll content of photosynthesis carriers as the algae was subjected to exposure stress by difenoconazole, difenoconazole-alcohol, and difenoconazole-alcohol enantiomer, it was concluded that difenoconazole was more toxic to Selenastrum capricornutum than difenoconazole-alcohol, and the toxicity of chiral difenoconazole-alcohol was regarded as S-(-)-enantiomer> racemate> R-(+)-enantiomer.
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  • 收稿日期:  2024-06-18
庄吉阳, 吴慧珍, 钱鸣蓉, 金哲雄, 盛颖霏, 章虎, 王军良, 孙娜波. 苯醚甲环唑和手性苯醚甲环唑醇对近头状尖孢藻的胁迫效应[J]. 生态毒理学报, 2024, 19(6): 335-343. doi: 10.7524/AJE.1673-5897.20240618001
引用本文: 庄吉阳, 吴慧珍, 钱鸣蓉, 金哲雄, 盛颖霏, 章虎, 王军良, 孙娜波. 苯醚甲环唑和手性苯醚甲环唑醇对近头状尖孢藻的胁迫效应[J]. 生态毒理学报, 2024, 19(6): 335-343. doi: 10.7524/AJE.1673-5897.20240618001
shu
ZHUANG Jiyang, WU Huizhen, QIAN Mingrong, JIN Zhexiong, SHENG Yingfei, ZHANG Hu, WANG Junliang, SUN Nabo. The Stress Effects of Difenoconazole and Chiral Difenoconazole-alcohol on Selenastrum capricornutum[J]. Asian journal of ecotoxicology, 2024, 19(6): 335-343. doi: 10.7524/AJE.1673-5897.20240618001
Citation: ZHUANG Jiyang, WU Huizhen, QIAN Mingrong, JIN Zhexiong, SHENG Yingfei, ZHANG Hu, WANG Junliang, SUN Nabo. The Stress Effects of Difenoconazole and Chiral Difenoconazole-alcohol on Selenastrum capricornutum[J]. Asian journal of ecotoxicology, 2024, 19(6): 335-343. doi: 10.7524/AJE.1673-5897.20240618001
shu

苯醚甲环唑和手性苯醚甲环唑醇对近头状尖孢藻的胁迫效应

    通讯作者: 王军良(1978—)，男，博士，教授，主要研究方向为超亚临界流体、高浓度有机废水处理与资源化和二氧化碳地质封存。E-mail:upupwang@zjut.edu.cn;  孙娜波(1975-),女,博士,教授,主要研究方向为有机合成。E-mail:sunnabo@zjsru.edu.cn
    作者简介: 庄吉阳(2000—)，男，硕士研究生，研究方向为环境毒理学，E-mail:1248448000@qq.com
  • 1. 浙江工业大学环境学院, 杭州 310032;
  • 2. 浙江树人学院生物与环境工程学院, 杭州 310015;
  • 3. 浙江树人学院交叉科学研究院, 杭州 310015;
  • 4. 绿城农科检测技术有限公司, 杭州 310052;
  • 5. 浙江工业大学绍兴研究院, 绍兴 312000
  • 收稿日期:  2024-06-18
基金项目:

浙江省“三农九方”农业科技协作计划项目(2024SNJF042)

摘要: 本研究以近头状尖孢藻为受试对象，通过测定半数有效浓度(EC50)、光合色素水平、丙二醛(MDA)含量及抗氧化酶活性，评估了苯醚甲环唑及其降解产物苯醚甲环唑醇对藻的生物毒性，重点分析了手性苯醚甲环唑醇对藻生物毒性的立体选择性差异。本文所测苯醚甲环唑和苯醚甲环唑醇96 h的EC50值分别为0.67 mg·L-1和1.74 mg·L-1，R-(+)-苯醚甲环唑醇和S-(-)-苯醚甲环唑醇96 h的EC50值分别为2.74 mg·L-1和1.68 mg·L-1。同时基于藻受苯醚甲环唑、苯醚甲环唑醇和苯醚甲环唑醇对映体胁迫后，氧化损伤相关参数的变化趋势以及光合作用载体叶绿素含量的程度变化趋势，得出苯醚甲环唑对近头状尖孢藻的毒性强于苯醚甲环唑醇，手性苯醚甲环唑醇对藻的胁迫表现为S-(-)-对映体＞消旋体＞R-(+)-对映体。

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