利用大型溞评估离子液体[C8mim]Cl生物毒性初探
Preliminary study on the biotoxicity evaluation of[C8mim]Cl by using Daphnia magna
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摘要: 为评估1-辛基-3-甲基咪唑氯盐（[C8mim]Cl）的生物毒性，本研究以大型溞（Daphnia magna）为实验对象，通过急性毒性实验分析了不同日龄大型溞对[C8mim]Cl的敏感性，并以存活率、产溞能力和蜕皮次数为指标研究了[C8mim]Cl对大型溞的慢性毒性效应，这将为评估离子液体的毒性效应提供科学依据.结果表明，[C8mim]Cl对1、2、4日龄大型溞的48 h半致死浓度（LC50）分别为208.9、182.7、178.6 μg·L-1，表明随着大型溞日龄的增加，[C8mim]Cl对大型溞的毒性效应逐渐增大并趋于稳定.此外，[C8mim]Cl对大型溞生活史中的初次产溞时间、总产溞数目、总产溞次数和蜕皮次数等影响不显著，但显著影响大型溞的存活率和初次产溞个数，因此，大型溞的存活率和初次产溞个数是评估[C8mim]Cl对大型溞慢性毒性效应的两个较为敏感的指标.
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关键词:
- 大型溞 /
- 1-辛基-3-甲基咪唑氯盐 /
- 急性毒性 /
- 慢性毒性
Abstract: In order to evaluate the biotoxicity of 1-octyl-3-methylimidazolium chloride ([C8mim]Cl) to Daphnia magna, the sensitivity of D. magna aged different days to[C8mim]Cl was studied through acute toxicity experiments. The survival rate, capacity of producing, and exuviate times were used as indicators to investigate the chronic toxic effects of[C8mim]Cl on D. magna, which would provide scientific evidences for further evaluating the toxic effects of ionic liquids. The results showed that the 48 h-LC50 values of[C8mim]Cl to D. magna aged 1, 2 and 4 days macrophages were 208.9, 182.7, 178.6 μg·L-1, respectively, indicating that the toxic effects of[C8mim]Cl on D. magna increased as the day-age D. magna increased and then stabilized. Moreover, it was showed that[C8mim]Cl had no significant effects on the first-generation time, total number and times, and total exuviate times of Daphnia magna, but the ionic liquid significantly inhibited the survival rate and the the first-generation number of Daphnia magna, suggesting that the survival rate and the first-generation number of Daphnia magna, would be the two sensitive indicators for evaluating the chronic toxic effects of[C8mim]Cl on D. magna.-
Key words:
- Daphnia magna /
- 1-octyl-3-methylimidazolium chloride /
- acute toxicity /
- chronic toxicity
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