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氨基改性聚苯乙烯对秀丽隐杆线虫的神经毒性及其作用机制

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郑友平, 彭远锋, 张双, 林锡华. 氨基改性聚苯乙烯对秀丽隐杆线虫的神经毒性及其作用机制[J]. 生态毒理学报, 2024, 19(6): 274-282. doi: 10.7524/AJE.1673-5897.20241018002
引用本文: 郑友平, 彭远锋, 张双, 林锡华. 氨基改性聚苯乙烯对秀丽隐杆线虫的神经毒性及其作用机制[J]. 生态毒理学报, 2024, 19(6): 274-282. doi: 10.7524/AJE.1673-5897.20241018002
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ZHENG Youping, PENG Yuanfeng, ZHANG Shuang, LIN Xihua. Neurotoxicity of Amino-modified Polystyrene and Its Mechanisms in Caenorhabditis elegans[J]. Asian journal of ecotoxicology, 2024, 19(6): 274-282. doi: 10.7524/AJE.1673-5897.20241018002
Citation: ZHENG Youping, PENG Yuanfeng, ZHANG Shuang, LIN Xihua. Neurotoxicity of Amino-modified Polystyrene and Its Mechanisms in Caenorhabditis elegans[J]. Asian journal of ecotoxicology, 2024, 19(6): 274-282. doi: 10.7524/AJE.1673-5897.20241018002
shu

氨基改性聚苯乙烯对秀丽隐杆线虫的神经毒性及其作用机制

  • 1. 广东新泓环境科技有限公司, 深圳 518071;

  • 2. 生态环境部华南环境科学研究所, 广州 510655

    • 作者简介: 郑友平(1990—)，男，硕士研究生，研究方向为土壤生态环境风险与修复治理，E-mail:895942073@qq.com
    通讯作者: 林锡华(1994-),男,学士,工程师,主要研究方向为环境健康。E-mail:linxihua714@163.com
  • 基金项目:

    国家自然科学基金资助项目(42177420)

  • 中图分类号: X171.5

Neurotoxicity of Amino-modified Polystyrene and Its Mechanisms in Caenorhabditis elegans

  • 1. Guangdong Xinhong Environmental Technology Co., Ltd., Shenzhen 518071, China;

  • 2. South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, China

    • Corresponding author: LIN Xihua, linxihua714@163.com
  • Fund Project:

  • 摘要: 聚苯乙烯微塑料(PS)广泛存在于各种环境介质中，且对生物体会造成多种毒性效应。然而，氨基修饰PS(PS-NH2)的神经毒性及其作用机制尚不明确。本研究以秀丽隐杆线虫为模式生物，以运动行为、神经元发育、神经递质水平及其相关基因表达作为评估终点，探讨环境浓度下(0.1~100 μg·L-1)PS与PS-NH2的神经毒性及神经传递机制。实验结果显示，与原始PS相比，浓度为1 μg·L-1的PS-NH2急性暴露即显著降低秀丽线虫的头部摆动与身体弯曲频率，表明PS-NH2具有更强的神经毒性。10~100 μg·L-1的PS-NH2急性暴露显著改变多巴胺能与5-羟色胺能神经元的荧光强度。与此同时，秀丽线虫体内的多巴胺与5-羟色胺含量均显著降低，且dop-1、dat-1与tph-1等相关基因的表达水平显著下调。结果表明，PS-NH2暴露会造成多巴胺能和5-羟色胺能神经元的损伤，可能影响多巴胺和5-羟色胺的神经传递，进而对秀丽线虫造成神经毒性效应。本研究为揭示改性微塑料的潜在风险及其作用机制提供了新的见解。
    Abstract: Polystyrene microplastics (PS) are widespread across various environmental media and pose potential toxic risks. However, the neurotoxicity and mechanisms of amino-modified PS (PS-NH2) remain poorly understood. In this study, Caenorhabditis elegans was used as a model organism to evaluate the neurotoxic effects of environmentally relevant concentrations (0.1-100 μg·L-1) of PS and PS-NH2 through endpoints including locomotion behavior, neuronal development, neurotransmitter levels, and gene expression. Results showed that acute exposure to 1 μg·L-1 of PS-NH2 significantly reduced head thrashing and body bending frequency in C. elegans, indicating enhanced neurotoxicity compared to PS. At doses of 10-100 μg·L-1, PS-NH2 exposure significantly altered the fluorescence intensity of dopaminergic and serotonergic neurons, and the levels of neurotransmitters, such as dopamine and serotonin, were notably decreased. Additionally, PS-NH2 exposure significantly decreased the expression of neurotransmission-related genes, including dop-1, dat-1, and tph-1. These findings suggest that PS-NH2 causes damage to dopaminergic and serotonergic neurons, potentially disrupting dopamine and serotonin neurotransmission and exerting neurotoxic effects on Caenorhabditis elegans. This study provides new insights into the potential risks and underlying mechanisms of modified microplastics in environmental systems.
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  • 收稿日期:  2024-10-18
郑友平, 彭远锋, 张双, 林锡华. 氨基改性聚苯乙烯对秀丽隐杆线虫的神经毒性及其作用机制[J]. 生态毒理学报, 2024, 19(6): 274-282. doi: 10.7524/AJE.1673-5897.20241018002
引用本文: 郑友平, 彭远锋, 张双, 林锡华. 氨基改性聚苯乙烯对秀丽隐杆线虫的神经毒性及其作用机制[J]. 生态毒理学报, 2024, 19(6): 274-282. doi: 10.7524/AJE.1673-5897.20241018002
shu
ZHENG Youping, PENG Yuanfeng, ZHANG Shuang, LIN Xihua. Neurotoxicity of Amino-modified Polystyrene and Its Mechanisms in Caenorhabditis elegans[J]. Asian journal of ecotoxicology, 2024, 19(6): 274-282. doi: 10.7524/AJE.1673-5897.20241018002
Citation: ZHENG Youping, PENG Yuanfeng, ZHANG Shuang, LIN Xihua. Neurotoxicity of Amino-modified Polystyrene and Its Mechanisms in Caenorhabditis elegans[J]. Asian journal of ecotoxicology, 2024, 19(6): 274-282. doi: 10.7524/AJE.1673-5897.20241018002
shu

氨基改性聚苯乙烯对秀丽隐杆线虫的神经毒性及其作用机制

    通讯作者: 林锡华(1994-),男,学士,工程师,主要研究方向为环境健康。E-mail:linxihua714@163.com
    作者简介: 郑友平(1990—)，男，硕士研究生，研究方向为土壤生态环境风险与修复治理，E-mail:895942073@qq.com
  • 1. 广东新泓环境科技有限公司, 深圳 518071;
  • 2. 生态环境部华南环境科学研究所, 广州 510655
  • 收稿日期:  2024-10-18
基金项目:

国家自然科学基金资助项目(42177420)

摘要: 聚苯乙烯微塑料(PS)广泛存在于各种环境介质中，且对生物体会造成多种毒性效应。然而，氨基修饰PS(PS-NH2)的神经毒性及其作用机制尚不明确。本研究以秀丽隐杆线虫为模式生物，以运动行为、神经元发育、神经递质水平及其相关基因表达作为评估终点，探讨环境浓度下(0.1~100 μg·L-1)PS与PS-NH2的神经毒性及神经传递机制。实验结果显示，与原始PS相比，浓度为1 μg·L-1的PS-NH2急性暴露即显著降低秀丽线虫的头部摆动与身体弯曲频率，表明PS-NH2具有更强的神经毒性。10~100 μg·L-1的PS-NH2急性暴露显著改变多巴胺能与5-羟色胺能神经元的荧光强度。与此同时，秀丽线虫体内的多巴胺与5-羟色胺含量均显著降低，且dop-1、dat-1与tph-1等相关基因的表达水平显著下调。结果表明，PS-NH2暴露会造成多巴胺能和5-羟色胺能神经元的损伤，可能影响多巴胺和5-羟色胺的神经传递，进而对秀丽线虫造成神经毒性效应。本研究为揭示改性微塑料的潜在风险及其作用机制提供了新的见解。

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