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郝杰威, 何再华, 周长芳, 田兴军. 高蛋白含量餐厨垃圾水解真菌的筛选和应用[J]. 环境工程学报, 2018, 12(1): 286-293. doi: 10.12030/j.cjee.201703054
引用本文: 郝杰威, 何再华, 周长芳, 田兴军. 高蛋白含量餐厨垃圾水解真菌的筛选和应用[J]. 环境工程学报, 2018, 12(1): 286-293. doi: 10.12030/j.cjee.201703054
shu
HAO Jiewei, HE Zaihua, ZHOU Changfang, TIAN Xingjun. Screening and application of fungi on hydrolysis of high-concentrated protein food waste[J]. Chinese Journal of Environmental Engineering, 2018, 12(1): 286-293. doi: 10.12030/j.cjee.201703054
Citation: HAO Jiewei, HE Zaihua, ZHOU Changfang, TIAN Xingjun. Screening and application of fungi on hydrolysis of high-concentrated protein food waste[J]. Chinese Journal of Environmental Engineering, 2018, 12(1): 286-293. doi: 10.12030/j.cjee.201703054
shu

高蛋白含量餐厨垃圾水解真菌的筛选和应用

  • 1.

    南京大学生命科学学院,南京 210046

  • 2.

    南京恩皆优生物科技有限公司,南京210046

  • 基金项目:

    国家水体污染控制与治理科技重大专项(2012ZX07204004-003)

    教育部博士点基金项目(20110091110018)

Screening and application of fungi on hydrolysis of high-concentrated protein food waste

  • 1.

    School of Life Sciences, Nanjing University, Nanjing 210046, China

  • 2.

    Nanjing Enjoy Biotechnology Co.Ltd., Nanjing 210046, China

  • Fund Project:

  • 摘要: 从足癣患者病灶部位分离及筛选出12株真菌,其中4株真菌在蛋白质培养基和淀粉水解培养基菌落水解圈 直径与菌落直径比(D/d) 表现突出。经ITS-5.8S rDNA鉴定分别为Tritirachium oryzae,Paecilomyces sp.,Aspergillus puniceus,Septoriella oudemansii。优化4株真菌的最适发酵温度,并选出优良菌株用于高蛋白含量餐厨垃圾(A)降解。液体发酵结果表明,温度和菌株对游离氨基氮和还原糖产量有显著影响,47 ℃为最适发酵温度,并且Aspergillus puniceus、Septoriella oudemansii显著优于其他真菌。因此,分别接种Aspergillus puniceus,Septoriella oudemansii以及Aspergillus puniceus和Septoriella oudemansii复合菌株到餐厨垃圾(B),预先进行固态发酵,5 d后进行液态发酵。24 h后发酵液中还原糖的浓度达到最大值,分别为12 080、9 832和13 123 mg·L-1,发酵108 h后游离氨基氮浓度达到最大值,分别为3 660、2 821和3 375 mg·L-1。研究表明,Aspergillus puniceus和Septoriella oudemansii复合菌株对还原糖产量有一定的促进作用,但是不利于游离氨基氮的产生。Aspergillus puniceus为最佳菌种,可以应用于高蛋白含量餐厨垃圾的生物降解和高附加值产品的开发等相关方面的研究。
    Abstract: 12 fungi were isolated and screened from athlete’s foot, known medically as tinea pedis, and 4 fungi with larger ratios of the diameter of the hydrolysis circle to the diameter of the colony(D/d), which could effectively degrade protein and starch were identified by ITS-5.8S rDNA as Tritirachium oryzae, Paecilomyces sp., Aspergillus puniceus and Septoriella oudemansii. In this paper, the optimal temperature and better strains for submerged fermentation were investigated using high-concentrated protein food waste A. The results showed that the yields of reducing sugar and free amino nitrogen could be greatly influenced by different temperature and strains, and 47 ℃ was the optimal temperature, and the yields of reducing sugar and free amino nitrogen of Aspergillus puniceus and Septoriella oudemansii were significantly higher than the rest. Then, solid state fermentation was performed for fungal solid mashes for 5 days using food waste B inoculated with either Aspergillus puniceus or Septoriella oudemansii or mixed Aspergillus puniceus and Septoriella oudemansii. Submerged fermentation was performed inoculated with fungal solid mashes. Experiments revealed that the highest concentration of reducing sugar were obtained at 24 h, 12 080, 9 832 and 13 123 mg·L-1, and the maximum yields of free amino nitrogen accumulated at 108 h, 3 660, 2 821 and 3 375 mg·L-1, respectively. Mixed Aspergillus puniceus and Septoriella oudemansii could accelerate the yields of reducing sugar, but do harm to the production of free amino nitrogen. Aspergillus puniceus could be used in the research of high-concentrated protein food waste for high-added value products.
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  • 刊出日期:  2018-01-14
郝杰威, 何再华, 周长芳, 田兴军. 高蛋白含量餐厨垃圾水解真菌的筛选和应用[J]. 环境工程学报, 2018, 12(1): 286-293. doi: 10.12030/j.cjee.201703054
引用本文: 郝杰威, 何再华, 周长芳, 田兴军. 高蛋白含量餐厨垃圾水解真菌的筛选和应用[J]. 环境工程学报, 2018, 12(1): 286-293. doi: 10.12030/j.cjee.201703054
shu
HAO Jiewei, HE Zaihua, ZHOU Changfang, TIAN Xingjun. Screening and application of fungi on hydrolysis of high-concentrated protein food waste[J]. Chinese Journal of Environmental Engineering, 2018, 12(1): 286-293. doi: 10.12030/j.cjee.201703054
Citation: HAO Jiewei, HE Zaihua, ZHOU Changfang, TIAN Xingjun. Screening and application of fungi on hydrolysis of high-concentrated protein food waste[J]. Chinese Journal of Environmental Engineering, 2018, 12(1): 286-293. doi: 10.12030/j.cjee.201703054
shu

高蛋白含量餐厨垃圾水解真菌的筛选和应用

  • 1. 南京大学生命科学学院,南京 210046
  • 2. 南京恩皆优生物科技有限公司,南京210046
基金项目:

国家水体污染控制与治理科技重大专项(2012ZX07204004-003)

教育部博士点基金项目(20110091110018)

摘要: 从足癣患者病灶部位分离及筛选出12株真菌,其中4株真菌在蛋白质培养基和淀粉水解培养基菌落水解圈 直径与菌落直径比(D/d) 表现突出。经ITS-5.8S rDNA鉴定分别为Tritirachium oryzae,Paecilomyces sp.,Aspergillus puniceus,Septoriella oudemansii。优化4株真菌的最适发酵温度,并选出优良菌株用于高蛋白含量餐厨垃圾(A)降解。液体发酵结果表明,温度和菌株对游离氨基氮和还原糖产量有显著影响,47 ℃为最适发酵温度,并且Aspergillus puniceus、Septoriella oudemansii显著优于其他真菌。因此,分别接种Aspergillus puniceus,Septoriella oudemansii以及Aspergillus puniceus和Septoriella oudemansii复合菌株到餐厨垃圾(B),预先进行固态发酵,5 d后进行液态发酵。24 h后发酵液中还原糖的浓度达到最大值,分别为12 080、9 832和13 123 mg·L-1,发酵108 h后游离氨基氮浓度达到最大值,分别为3 660、2 821和3 375 mg·L-1。研究表明,Aspergillus puniceus和Septoriella oudemansii复合菌株对还原糖产量有一定的促进作用,但是不利于游离氨基氮的产生。Aspergillus puniceus为最佳菌种,可以应用于高蛋白含量餐厨垃圾的生物降解和高附加值产品的开发等相关方面的研究。

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