N-乙烯基吡咯烷酮降解菌的筛选、降解特性及其竹炭固定化

郑菲菲; 胡江; 辛华东; 陈立伟; 蔡天明

doi:10.12030/j.cjee.201505199

环境工程学报

N-乙烯基吡咯烷酮降解菌的筛选、降解特性及其竹炭固定化

, , , ,

郑菲菲, 胡江, 辛华东, 陈立伟, 蔡天明. N-乙烯基吡咯烷酮降解菌的筛选、降解特性及其竹炭固定化[J]. 环境工程学报, 2016, 10(10): 5593-5599. doi: 10.12030/j.cjee.201505199

引用本文:

ZHENG Feifei, HU Jiang, XIN Huadong, CHEN Liwei, CAI Tianming. Isolation and degradation characteristics of a N-vinylpyrrolidone-degrading strain ZF1 and its immobilization on bamboo-based carbon[J]. Chinese Journal of Environmental Engineering, 2016, 10(10): 5593-5599. doi: 10.12030/j.cjee.201505199

Citation:

ZHENG Feifei, HU Jiang, XIN Huadong, CHEN Liwei, CAI Tianming. Isolation and degradation characteristics of a N-vinylpyrrolidone-degrading strain ZF1 and its immobilization on bamboo-based carbon[J]. Chinese Journal of Environmental Engineering, 2016, 10(10): 5593-5599. doi: 10.12030/j.cjee.201505199

N-乙烯基吡咯烷酮降解菌的筛选、降解特性及其竹炭固定化

1.
南京农业大学资源与环境科学学院环境工程系, 南京 210095
基金项目:
江苏省太湖水环境综合治理科研课题（TH2014210，TH2013210）
中图分类号: X703

Isolation and degradation characteristics of a N-vinylpyrrolidone-degrading strain ZF1 and its immobilization on bamboo-based carbon

1.
Department of Environmental Engineering, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China
Fund Project:

摘要: 从活性污泥中分离筛选得到1株N-乙烯基吡咯烷酮（NVP）高效降解菌株ZF1，根据菌株ZF1的形态特征、生理生化特性和16S rRNA基因序列同源性分析，将其初步鉴定为产脲节杆菌（Arthrobacter ureafaciens）。菌株ZF1能以NVP为唯一碳、氮源进行生长，并在60 h内完全降解200 mg·L-1的NVP。菌株ZF1降解NVP的最适环境条件为温度30℃，初始pH 7.0，NaCl浓度7 g·L-1。为了更好地实现其在实际废水中的应用，将菌株ZF1固定到竹炭上，扫描电镜观察表明ZF1能很好的附着在竹炭表面，且固定菌对NVP的降解效率明显高于游离菌。游离菌和固定菌对NVP的降解均符合一级动力学模型。重复利用5次后，固定菌对NVP的降解率仍能达到98%。
- N-乙烯基吡咯烷酮 /
- 分离鉴定 /
- Arthrobacter ureafaciens /
- 降解特性 /
- 竹炭 /
- 固定化
Abstract: N-vinylpyrrolidone (NVP) is a common raw material widely used in many industries and belongs to the nitrogen heterocyclic compounds (NHCs).Biological degradation was demonstrated as the most cost effective and environmentally friendly way to remove nitrogen heterocyclic pollutants from the environment.In the current study,a highly effective NVP-degrading bacteria,designated ZF1,was isolated from activated sludge.Based on its morphological,physiological,and biochemical properties and 16S rRNA gene sequence,the strain was identified as Arthrobacter ureafaciens.Strain ZF1 could use NVP as its sole carbon and nitrogen sources,degrading 200 mg·L-1 NVP to a nondetectable level within 60 h.The optimal environmental conditions for the degradation of NVP were 30℃,initial pH of 7.0,and NaCl of 7 g·L-1.For its application in real wastewater treatment,strain ZF1 was immobilized onto bamboo-based carbon to enhance the NVP degradation efficiency.SEM observation indicated that strain ZF1 cells were attached well on the surface and pores of the bamboo-based carbon.The degradation kinetics of NVP by free and immobilized cells followed the first-order model.After it was reused 5 times,NVP degradation efficiency was maintained at 98%.
- N-vinylpyrrolidone /
- isolation and identification /
- Arthrobacter ureafaciens /
- degradation characteristics /
- bamboo-based carbon /
- immobilization

[1]	ZHAO Cui,ZHANG Yin,LI Xiaobao,et al.Biodegradation of carbazole by the seven Pseudomonas sp.strains and their denitrification potential.Journal of Hazardous Materials,2011,190(1/2/3):253-259
[2]	ZHAO Hong,LIANG Lijun,LIU Hongwen.Fast photo-catalytic degradation of pyridine in nano aluminum oxide suspension systems.Journal of Environmental Sciences,2011,23(S):S156-S158
[3]	BAI Yaohui,SUN Qinghua,XING Rui,et al.Removal of pyridine and quinoline by bio-zeolite composed of mixed degrading bacteria and modified zeolite.Journal of Hazardous Materials,2010,181(1/2/3):916-922
[4]	LI Yongmei,GU Guowei,ZHAO Jianfu,et al.Anoxic degradation of nitrogenous heterocyclic compounds by acclimated activated sludge.Process Biochemistry,2001,37(1):81-86
[5]	KLIMISCH H.J.,DECKARDT K.,GEMBARDT C.,et al.Subchronic inhalation and oral toxicity of N-vinylpyrrolidone-2.Studies in rodents.Food and Chemical Toxicology,1997,35(10/11):1061-1074
[6]	MEHDINIA A.,GHASSEMPOUR A.,RAFATI H.,et al.Determination of N-vinyl-2-pyrrolidone and N-methyl-2-pyrrolidone in drugs using polypyrrole-based headspace solid-phase microextraction and gas chromatography-nitrogen-phosphorous detection.Analytica Chimica Acta,2007,587(1):82-88
[7]	KLIMISCH H.J.,DECKARDT K.,GEMBARDT C.,et al.Long-term inhalation toxicity of N-vinylpyrrolidone-2 vapours.Studies in rats.Food and Chemical Toxicology,1997,35(10/11):1041-1060
[8]	ABDUL SALAM J.,DAS N.Enhanced biodegradation of lindane using oil-in-water bio-microemulsion stabilized by biosurfactant produced by a new yeast strain,Pseudozyma VITJzN01.Journal of Microbiology and Biotechnology,2013,23(11):1598-1609
[9]	OLUKANNI O.D.,OSUNTOKI A.A.,AWOTULA A.O.,et al.Decolorization of Dyehouse Effluent and Biodegradation of Congo Red by Bacillus thuringiensis RUN1.Journal of Microbiology and Biotechnology,2013,23(6):843-849
[10]	ROBATJAZI S.M.,SHOJAOSADATI S.A.,KHALILZADEH R.,et al.Continuous biodegradation of parathion by immobilized Sphingomonas sp.in magnetically fixed-bed bioreactors and evaluation of the enzyme stability of immobilized bacteria.Biotechnology Letters,2013,35(1):67-73
[11]	CHENG Zhongqin,CHEN Mei,XIE Liqun,et al.Bioaugmentation of a sequencing batch biofilm reactor with Comamonas testosteroni and Bacillus cereus and their impact on reactor bacterial communities.Biotechnology Letters,2015,37(2):367-373
[12]	LI Chunyan,LI Yue,CHENG Xiaosong,et al.Immobilization of Rhodococcus rhodochrous BX2 (an acetonitrile-degrading bacterium) with biofilm-forming bacteria for wastewater treatment.Bioresource Technology,2013,131:390-396
[13]	PARKHURST J.D.,DRYDEN F.D.,MCDERMOTT G.N.,et al.Pomona activated carbon pilot plant.Journal (Water Pollution Control Federation),1967,39(10):R70-R81
[14]	LIAO Peng,ISMAEL Z.M.,ZHANG Wenbiao,et al.Adsorption of dyes from aqueous solutions by microwave modified bamboo charcoal.Chemical Engineering Journal,2012,195-196:339-346
[15]	QIAO Lin,WEN Donghui,WANG Jianlong.Biodegradation of pyridine by Paracoccus sp.KT-5 immobilized on bamboo-based activated carbon.Bioresource Technology,2010,101(14):5229-5234
[16]	LIU Yan,GAN Li,CHEN Zuliang,et al.Removal of nitrate using Paracoccus sp.YF1 immobilized on bamboo carbon.Journal of Hazardous Materials,2012,229-230:419-425
[17]	东秀珠,蔡妙英.常见细菌系统鉴定手册.北京:科学出版社,2001
[18]	周博如.阿特拉津降解菌的分离、鉴定及特性研究.哈尔滨:东北林业大学博士学位论文,2011 ZHOU Boru.Isolation and identification of atrazine-degrading bacteria and degradation characterization.Harbin:Doctor Dissertation of Northeast Forestry University,2011(in Chinese)
[19]	BAE H.S.,LEE J.M.,LEE S.T.Biodegradtion of 4-chrophenol via a hydroquinone pathway by Arthrobacter ureafaciens CPR706.FEMS Microbiology Letters,1996,145(1):125-129
[20]	周玉央,陈东之,金小君,等.食油假单胞菌DT4菌株对四氢呋喃(THF)的降解特性.环境科学,2011,32(1):266-271 ZHOU Yuyang,CHEN Dongzhi,JIN Xiaojun,et al.Characteristics of tetrahydrofuran degradation by Pseudomonas oleovorans DT4.Environmental Science,2011,32(1):266-271(in Chinese)
[21]	於建明,刘靓,蔡文吉,等.一株苯乙烯高效降解菌的分离鉴定及降解特性研究.中国环境科学,2014,34(6):1564-1571 YU Jianming,LIU Liang,CAI Wenji,et al.Identification and characteristics of a Pseudomonas putida strain with the capable of efficiently degrade styrene.China Environmental Science,2014,34(6):1564-1571(in Chinese)
[22]	董滨,王凤花,林爱军,等.乙草胺降解菌A-3的筛选及其降解特性.环境科学,2011,32(2):542-547 DONG Bin,WANG Fenghua,LIN Aijun,et al.Isolation and degradation characteristics of acetochlor-degrading strain A-3.Environmental Science,2011,32(2):542-547(in Chinese)

点击查看大图

计量

文章访问数: 1484
HTML全文浏览数: 985
PDF下载数: 317
施引文献: 0

N-乙烯基吡咯烷酮降解菌的筛选、降解特性及其竹炭固定化

1. 南京农业大学资源与环境科学学院环境工程系, 南京 210095

收稿日期: 2015-06-19

基金项目:

江苏省太湖水环境综合治理科研课题（TH2014210，TH2013210）

关键词:

摘要: 从活性污泥中分离筛选得到1株N-乙烯基吡咯烷酮（NVP）高效降解菌株ZF1，根据菌株ZF1的形态特征、生理生化特性和16S rRNA基因序列同源性分析，将其初步鉴定为产脲节杆菌（Arthrobacter ureafaciens）。菌株ZF1能以NVP为唯一碳、氮源进行生长，并在60 h内完全降解200 mg·L-1的NVP。菌株ZF1降解NVP的最适环境条件为温度30℃，初始pH 7.0，NaCl浓度7 g·L-1。为了更好地实现其在实际废水中的应用，将菌株ZF1固定到竹炭上，扫描电镜观察表明ZF1能很好的附着在竹炭表面，且固定菌对NVP的降解效率明显高于游离菌。游离菌和固定菌对NVP的降解均符合一级动力学模型。重复利用5次后，固定菌对NVP的降解率仍能达到98%。

English Abstract

Isolation and degradation characteristics of a N-vinylpyrrolidone-degrading strain ZF1 and its immobilization on bamboo-based carbon

1. Department of Environmental Engineering, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China

Received Date: 2015-06-19

Fund Project:

Keywords:

Abstract: N-vinylpyrrolidone (NVP) is a common raw material widely used in many industries and belongs to the nitrogen heterocyclic compounds (NHCs).Biological degradation was demonstrated as the most cost effective and environmentally friendly way to remove nitrogen heterocyclic pollutants from the environment.In the current study,a highly effective NVP-degrading bacteria,designated ZF1,was isolated from activated sludge.Based on its morphological,physiological,and biochemical properties and 16S rRNA gene sequence,the strain was identified as Arthrobacter ureafaciens.Strain ZF1 could use NVP as its sole carbon and nitrogen sources,degrading 200 mg·L-1 NVP to a nondetectable level within 60 h.The optimal environmental conditions for the degradation of NVP were 30℃,initial pH of 7.0,and NaCl of 7 g·L-1.For its application in real wastewater treatment,strain ZF1 was immobilized onto bamboo-based carbon to enhance the NVP degradation efficiency.SEM observation indicated that strain ZF1 cells were attached well on the surface and pores of the bamboo-based carbon.The degradation kinetics of NVP by free and immobilized cells followed the first-order model.After it was reused 5 times,NVP degradation efficiency was maintained at 98%.

全文HTML

参考文献 (22)

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

N-乙烯基吡咯烷酮降解菌的筛选、降解特性及其竹炭固定化

Isolation and degradation characteristics of a N-vinylpyrrolidone-degrading strain ZF1 and its immobilization on bamboo-based carbon

计量

出版历程

N-乙烯基吡咯烷酮降解菌的筛选、降解特性及其竹炭固定化

English Abstract

Isolation and degradation characteristics of a N-vinylpyrrolidone-degrading strain ZF1 and its immobilization on bamboo-based carbon

全文HTML

目录