C/N比对ANAMMOX与反硝化协同脱氮性能影响及其动力学
Influence of C/N on nitrogen removal performance and kinetics characteristics of ANAMMOX/denitrification synergistic interaction
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摘要: 采用ASBR厌氧氨氧化(ANAMMOX)反应器,考察了不同C/N(NH4+-N)比时厌氧氨氧化与反硝化协同脱氮性能表现,并与无机环境下反应器的脱氮性能相比较。研究结果表明,C/N比决定了ANAMMOX/反硝化耦合反应的发展方向。当C/N4+-N和NO2--N的去除率分别为92%、95%、COD去除率大于96%,实现了氨氮及COD的同时去除;当C/N=1.33时,反硝化反应逐渐占据优势;当C/N>2.96时,反硝化作用成为主导反应,厌氧氨氧化反应受到明显抑制,氨氮去除率下降。采取批次实验方法研究了厌氧氨氧化与反硝化协同反应的动力学特性。用基质抑制动力学Haldane模型拟合不同基质浓度下的厌氧氨氧化活性,得到氨氮最大比增长速率为0.09 kg/(kg·d)(以VSS计),半饱和常数为8.4 mg/L、半抑制常数为1 198.2 mg/L;亚硝态氮最大比增长速率为0.27 kg/(kg·d)(以VSS计),半饱和常数为10.2 mg/L、半抑制常数为300.1 mg/L。采用Monod模型和Haldane模型分别拟合不同COD浓度和亚硝酸盐浓度下的反硝化性能,得到反硝化亚硝态氮最大比增长速率为0.2 kg/(kg VSS·d),半饱和常数为17.4 mg/L、半抑制常数为128.4 mg/L,COD半饱和常数为83.3 mg/L。Abstract: The effect of different C/N(NH4+-N) on nitrogen removal performance by ANAMMOX/denitrification synergistic interaction in anaerobic sequencing batch reactor was investigated. Apart from the ANAMMOX performance under inorganic conditions, the results indicate that C/N ratio determines the direction of development of the ANAMMOX/denitrification synergistic interaction. ANAMMOX is the dominant reaction when the ratio of C/N is less than 0.33. The optimal ratio of C/N is 0.67, and the ammonium and nitrite removal efficiencies were 92% and 95%, respectively. The removal efficiency of COD was also higher than 96%. When the ratio of C/N is higher than 1.33, denitrification reaction share the advantage gradually. When the ratio of C/N is higher than 2.96, denitrification becomes the dominant reaction, and the decreasing removal efficiencies of ammonium indicate the weakening of ANAMMOX reaction. The kinetic characteristics of ANAMMOX/denitrification synergistic interaction were studied through batch tests. The kinetic characteristics of ANAMMOX can be described by the Haldane model as ammonia and nitrite can both inhibit the ANAMMOX biomass. The kinetic parameters were determined, where the maximum specific growth rate of ammonia was 0.09 kg/(kg·d) (calculated as VSS). The half saturation constants and the half inhibition constants of ammonium were 8.4 mg/L and 1 198.2 mg/L, respectively. The maximum specific growth rate of nitrite was 0.27 kg/(kg·d)(calculated as VSS), and the half saturation constants and half inhibition constants for nitrite were 10.2 mg/L and 300.1 mg/L, respectively. The Monod model and the Haldane model were used to determine the kinetic characteristics of COD and nitrite, respectively. The results showed that the maximum specific growth rate of nitrite was 0.2 kg/(kg·d)(calculated as VSS). The half saturation constant of nitrite was 17.4 mg/L, and the half inhibition constant of nitrite was 128.4 mg/L. The half saturation constant of COD was 83.3 mg/L.
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Key words:
- ANAMMOX /
- denitrification /
- synergistic reaction /
- reaction kinetics /
- C/N
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