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Zhang Qingyuan, Ge Shijie, Jiang Zhao, Wang Zhigang, Zhang Ying. Optimization for degradation conditions of atrazine-degrading strain DNS10[J]. Chinese Journal of Environmental Engineering, 2013, 7(3): 1169-1174.
Citation: Zhang Qingyuan, Ge Shijie, Jiang Zhao, Wang Zhigang, Zhang Ying. Optimization for degradation conditions of atrazine-degrading strain DNS10[J]. Chinese Journal of Environmental Engineering, 2013, 7(3): 1169-1174.
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Optimization for degradation conditions of atrazine-degrading strain DNS10

  • 1.

    School of Resources & Environment, Northeast Agricultural University, Harbin 150030, China

  • Received Date: 07/01/2012
    Accepted Date: 17/11/2011
    Available Online: 18/03/2013
    Fund Project:
  • An atrazine-degrading strain, which grew with atrazine as the nitrogen source, was isolated from the top of black soil (0~10 cm) in cold area of long-term applying atrazine.The strain was designated as Arthrobacter sp. DNS10 according to the sequence of 16S rRNA. It is capable of removing 100 mg/L atrazine in medium about 99.41% within 24 h with 108 CFU/mL inoculation. The results of single factor analysis show the optimal conditions for growth and degradation ability of DNS10. The range of temperature is 25~35℃, pH is 5.0~8.0, medium salinity is 0.1%~2% and the maximum resistance concentration of atrazine is 1 200 mg/L. Furthermore, based on orthogonal experiments, the optimal combination scheme for the growth and degradation ability of DNS10 is that temperature is 30℃, pH is 7.5 and medium salinity is 0.5%. The primary and secondary order of environmental factors that influence the degradation ability of DNS10 is that: temperature＞salinity＞pH.
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Optimization for degradation conditions of atrazine-degrading strain DNS10

  • Received Date: 07/01/2012
  • Accepted Date: 17/11/2011
  • Available Online: 18/03/2013
Fund Project:

Abstract: An atrazine-degrading strain, which grew with atrazine as the nitrogen source, was isolated from the top of black soil (0~10 cm) in cold area of long-term applying atrazine.The strain was designated as Arthrobacter sp. DNS10 according to the sequence of 16S rRNA. It is capable of removing 100 mg/L atrazine in medium about 99.41% within 24 h with 108 CFU/mL inoculation. The results of single factor analysis show the optimal conditions for growth and degradation ability of DNS10. The range of temperature is 25~35℃, pH is 5.0~8.0, medium salinity is 0.1%~2% and the maximum resistance concentration of atrazine is 1 200 mg/L. Furthermore, based on orthogonal experiments, the optimal combination scheme for the growth and degradation ability of DNS10 is that temperature is 30℃, pH is 7.5 and medium salinity is 0.5%. The primary and secondary order of environmental factors that influence the degradation ability of DNS10 is that: temperature＞salinity＞pH.

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