2010 Volume 4 Issue 6
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Shen Guo, Li Yin. Total DNA extraction from activated sludge in a wastewater biotreatment system[J]. Chinese Journal of Environmental Engineering, 2010, 4(6): 1336-1340.
Citation: Shen Guo, Li Yin. Total DNA extraction from activated sludge in a wastewater biotreatment system[J]. Chinese Journal of Environmental Engineering, 2010, 4(6): 1336-1340.
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Total DNA extraction from activated sludge in a wastewater biotreatment system

  • 1.

    College of Environmental Science & Engineering, Donghua University, Shanghai 201620, China

  • Received Date: 13/05/2009
    Accepted Date: 23/04/2009
    Fund Project:
  • Effects of total DNA extraction from activated sludge with/without pretreatment were compared by three kinds of cell lysis methods, i.e. sonication irradiation, glass bead shaking and freezethawing process in an anaerobic-anoxic-aerobic denitrification-dephosphorization system. By detection of ultraviolet adsorption spectrum analysis and agarose gel electrophoresis, results showed that the DNA samples with high yield and purity were obtained by TENP and PBS pretreated, and 2%SDS and freeze-thawing lyzed, and PCR analysis was done directly without purification.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Total DNA extraction from activated sludge in a wastewater biotreatment system

  • Received Date: 13/05/2009
  • Accepted Date: 23/04/2009
Fund Project:

Abstract: Effects of total DNA extraction from activated sludge with/without pretreatment were compared by three kinds of cell lysis methods, i.e. sonication irradiation, glass bead shaking and freezethawing process in an anaerobic-anoxic-aerobic denitrification-dephosphorization system. By detection of ultraviolet adsorption spectrum analysis and agarose gel electrophoresis, results showed that the DNA samples with high yield and purity were obtained by TENP and PBS pretreated, and 2%SDS and freeze-thawing lyzed, and PCR analysis was done directly without purification.

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