Optimization for experimental condition of nitrate reductase in landfill
- Received Date: 28/05/2008
- Accepted Date: 29/04/2008
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Key words:
- landfill /
- nitrate reductase /
- optimization /
- orthogonal design /
- determination condition
Abstract: As one of the key enzymes of the nitrogen conversion in landfill,nitrate reductase(NR) played an important role in the course of nitrogen transfer in landfill.The study optimized the experimental condition of NR in landfill with the waste sample of bioreactor landfill and on the basis of the soil enzymology. The results showed that the best experimental conditions of NR in landfill were 30℃ of dried temperature, 2.0 mL and 0.5 mL of 1% KNO3 and glucose respectively, 7 min of evacuation time,20℃ of culture temperature and 36 h of culture period. The relatively standard deviation(RSD) is lower than 1.66% when NR in landfill was measured under these conditions. The analysis shows that the results are reliable and high precision.
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