GAO Yan, JI Jiayang, SUN Dezhi. Isolation and characteristic of dominant salt-tolerant strains from incineration leachate treatment[J]. Chinese Journal of Environmental Engineering, 2017, 11(9): 4944-4951. doi: 10.12030/j.cjee.201609051
Citation: GAO Yan, JI Jiayang, SUN Dezhi. Isolation and characteristic of dominant salt-tolerant strains from incineration leachate treatment[J]. Chinese Journal of Environmental Engineering, 2017, 11(9): 4944-4951. doi: 10.12030/j.cjee.201609051

Isolation and characteristic of dominant salt-tolerant strains from incineration leachate treatment

  • Received Date: 15/12/2016
    Accepted Date: 06/09/2016
    Available Online: 26/08/2017
    Fund Project:
  • Three dominant salt-tolerant strains (Strain1, Strain2 and Strain3) were isolated by modified Gibbons media from an EGSB reactor treating incineration leachate. The growth conditions of these three strains were investigated and the 16S rDNA information was documented. By using static tests, the effects of temperature and inocula on the COD removal efficiencies by the three strains were studied. Species construction was also conducted to enhancing COD removal. The experimental results showed that, Strain1, Strain2 and Strain3 belonged to genus Bacillus, Clostridium and Enterobacter, respectively. The optimal COD removal conditions of all three isolated strains was from 30 to 35℃ with a 10% inoculations. When the bacteria consortium of Strain1 and Strain2 with the inoculation ratio of 1:2 and influent leachate COD at 20 000 mg·L-1, the COD removal effiency reached as high as 54.3%. This inoculated combination was regarded as the optimal bacteria consortium among the three isolated strains.
  • [1] CHOU J D, WEY M Y, LIANG H H, et al. Biotoxicity evaluation of fly ash and bottom ash from different municipal solid waste incinerators[J]. Journal of Hazardous Materials, 2009, 168(1):197-202

    Google Scholar Pub Med

    [2] 中国环境保护产业协会城市生活垃圾处理委员会.我国城市生活垃圾处理行业2010年发展综述[J].中国环保产业, 2011(4):32-37

    Google Scholar Pub Med

    [3] 林文华.垃圾焚烧电厂二恶英的产生及控制[J].广东化工,2016,43(14):179-181

    Google Scholar Pub Med

    [4] 李华,赵由才,王罗春.垃圾堆酵对焚烧厂垃圾热值的影响[J].上海环境科学,2000, 19(2):89-91

    Google Scholar Pub Med

    [5] ZHANG W L, ZHANG L, LI A M. Anaerobic co-digestion of food waste with MSW incineration plant fresh leachate:Process performance and synergistic effects[J]. Chemical Engineering Journal, 2015,259:795-805

    Google Scholar Pub Med

    [6] 叶杰旭,穆永杰,孙德智,等.生活垃圾焚烧厂沥滤液处理技术研究进展[J]. 环境科学与技术,2012, 35(S1):134-139

    Google Scholar Pub Med

    [7] LIU Z, DANG Y, SUN D Z, et al. Inhibitory effect of high NH4+-N concentration on anaerobic biotreatment of fresh leachate from a municipal solid waste incineration plant[J]. Waste Management, 2015, 43:188-195

    Google Scholar Pub Med

    [8] DANG Y, ZHANG R, SUN D Z, et al. Calcium effect on anaerobic biological treatment of fresh leachate with extreme high calcium concentration[J].International Biodeterioration & Biodegradation, 2015,95:76-83

    Google Scholar Pub Med

    [9] KUMAR G, BAKONVI P, KOBAYASHI T, et al. Enhancement of biofuel production via microbial augmentation:The case of dark fermentative hydrogen[J]. Renewable and Sustainable Energy Reviews, 2016, 57:879-891

    Google Scholar Pub Med

    [10] HESNAWI R, DAHMANI K, AL-SWAYAH A, et al. Biodegradation of municipal wastewater with local and commercial bacteria[J]. Procedia Engineering, 2014,70:810-814

    Google Scholar Pub Med

    [11] 全向春,刘佐才,范广裕,等.生物强化技术及其在废水治理中的应用[J]. 环境科学研究, 1999,12(2):22-27

    Google Scholar Pub Med

    [12] HERRERO M, STUCKEY D C. Bioaugmentation and its application in wastewater treatment:A review[J]. Chemosphere, 2015, 140:119-128

    Google Scholar Pub Med

    [13] RUNGROD J, KONSTANTINOS K,EAKALAK K. Immobilized-cell-augmented activated sludge process for treating wastewater containing hazardous compounds[J]. Water Environment Research, 2007, 79(5):461-471

    Google Scholar Pub Med

    [14] CHEN M X, FAN R, ZOU W H, et al. Bioaugmentation for treatment of full scale diethylene glycol monobutyl ether wastewater by Serratia sp. BDG-2[J]. Journal of Hazardous Materials, 2016, 309:20-26

    Google Scholar Pub Med

    [15] WENDEROTH D F, ROSENBROCK P, ABRAHAM W R, et al. Bacterial community dynamics during biostimulation and bioaugmentation experiments aiming at chlorobenzene degradation in groundwater[J]. Microbial Ecology, 2003, 46:161-176

    Google Scholar Pub Med

    [16] GUO L, JI M, DONG H Y, et al. Screening and degradation performances of dominant strains in high-salinity landfill leachate[J]. Applied Microbiology and Biotechnology, 2009, 84(2):357-364

    Google Scholar Pub Med

    [17] AGGELIS G, ICONOMOU D, CHRITOU M, et al. Phenolic removal in model olive oil mill wastewater using Pleyrotus ostreatus in bioreactor cultures and biological evaluation of the process[J]. Water Research, 2003, 37(16):3897-3904

    Google Scholar Pub Med

    [18] TAKENO K, YAMAOKA Y, SASAKI K. Treatment of oil-containing sewage wastewater using immobilized photosynthetic bacteria[J]. World Journal of Microbiology & Biotechnology, 2005, 21(8/9):1385-1391

    Google Scholar Pub Med

    [19] NAKASAKI K, SHOGO A, MIMOTO H. Inoculation of Pichia kudriavzevii RB1 degrades the organic acids present in raw compost material and accelerates composting[J]. Bioresource Technology, 2013, 144:521-528

    Google Scholar Pub Med

    [20] TRAN Q, MIMOTO H, NAKASAKI K. Inoculation of lactic acid bacterium accelerates organic matter degradation during composting[J]. International Biodeterioration & Biodegradation, 2015, 104:377-383

    Google Scholar Pub Med

    [21] 邱忠平,杨立中,刘丹,等.垃圾渗滤液COD降解菌株的筛选及其降解特性初探[J].四川环境, 2007, 26(1):5-8

    Google Scholar Pub Med

    [22] 邱忠平,杨立中,刘丹,等.优势菌株对垃圾渗滤液COD的降解特性[J].西南交通大学学报,2006, 41(2):264-268

    Google Scholar Pub Med

    [23] 叶杰旭,党岩,穆永杰,等.EGSB反应器处理城市生活垃圾沥滤液[J].环境工程学报,2012, 6(7):2321-2327

    Google Scholar Pub Med

    [24] DANG Y, LEI Y Q, SUN D Z, et al. Impact of fulvic acids on bio-methanogenic treatment of municipal solid waste incineration leachate[J]. Water Research, 2016, 106:71-78

    Google Scholar Pub Med

    [25] LEI Y Q, SUN D Z, DANG Y. Stimulation of methanogenesis in anaerobic digesters treating leachate from a municipal solid waste incineration plant with carbon cloth[J]. Bioresource Technology, 2016, 222:270-276

    Google Scholar Pub Med

    [26] 杜连祥,路福平.微生物学实验技术[M].北京:中国轻工业出版社,2005

    Google Scholar Pub Med

    [27] 党岩,张瑞,孙德智,等.EGSB处理垃圾焚烧渗沥液及其微生物群落变化[J].中国环境科学,2013,33(6):999-1004

    Google Scholar Pub Med

    [28] 党岩.垃圾焚烧厂渗沥液中富里酸对厌氧生物处理的影响及其降解和转化研究[D].北京:北京林业大学, 2016

    Google Scholar Pub Med

    [29] XU B, TIAN F X, HU C Y, et al. Chlorination of chlortoluron:Kinetics, pathways and chloroform formation[J]. Chemosphere, 2011, 83(7):909-916

    Google Scholar Pub Med

    [30] 林静,谢冰.复合微生物制剂在环境保护中的应用[J].上海化工,2004, 29(12):7-11

    Google Scholar Pub Med

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Isolation and characteristic of dominant salt-tolerant strains from incineration leachate treatment

Fund Project:

Abstract: Three dominant salt-tolerant strains (Strain1, Strain2 and Strain3) were isolated by modified Gibbons media from an EGSB reactor treating incineration leachate. The growth conditions of these three strains were investigated and the 16S rDNA information was documented. By using static tests, the effects of temperature and inocula on the COD removal efficiencies by the three strains were studied. Species construction was also conducted to enhancing COD removal. The experimental results showed that, Strain1, Strain2 and Strain3 belonged to genus Bacillus, Clostridium and Enterobacter, respectively. The optimal COD removal conditions of all three isolated strains was from 30 to 35℃ with a 10% inoculations. When the bacteria consortium of Strain1 and Strain2 with the inoculation ratio of 1:2 and influent leachate COD at 20 000 mg·L-1, the COD removal effiency reached as high as 54.3%. This inoculated combination was regarded as the optimal bacteria consortium among the three isolated strains.

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