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-. 开发心血管和神经发育指标作为鱼类胚胎毒性实验中的亚致死终点[J]. 生态毒理学报, 2018, 13(5):
开发心血管和神经发育指标作为鱼类胚胎毒性实验中的亚致死终点
Development of cardiovascular and neurodevelopmental metrics as sublethal endpoints for the fish embryo toxicity test
  
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中文摘要:
      黑头软口鲦的胚胎毒性实验(fish embryo toxicity, FET)是一个更加人性化的替代方法,因为年轻生物被认为在毒物暴露中受到的危害更小。FET实验草案没有包括毒性终点以预测亚致死有害结局,这限制了它的应用。研究者针对FET实验提出了亚致死毒性终点的开发以增进该方法的应用范围。本研究针对黑头软口鲦开发了用于以往未测定过的亚致死指标的方法(例如自主收缩频率和心率);考察了一些亚致死效应终点的响应情况,包括成长相关的(湿重、体长、生长相关基因表达),神经发育方面的(自主收缩频率和神经发育基因表达),心血管功能和发育(心包区面积、眼睛大小和心血管相关基因表达),这是指标均作为FET实验的额外指标,模式毒物为3,4-二氯苯胺。其中,体长、眼睛尺寸和心包区面积相比于其他终点响应更为敏感。建立长时间有害影响与这些终点变化的关系对全面评价这些指标的毒性预测能力十分必要。 精选自Julie C. Krzykwa, Alexis Olivas, Marlo K. Sellin Jeffries. Development of cardiovascular and neurodevelopmental metrics as sublethal endpoints for the fish embryo toxicity test. Environmental Toxicology and Chemistry,2018,37:2530-2541.
详情请见 https://doi.org/10.1002/etc.4212
  
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Julie C. Krzykwa, Alexis Olivas, Marlo K. Sellin Jeffries
英文摘要:
      The fathead minnow fish embryo toxicity (FET) test has been proposed as a more humane alternative to current toxicity testing methods, as younger organisms are thought to experience less distress during toxicant exposure. However, the FET test protocol does not include endpoints that allow for the prediction of sublethal adverse outcomes, limiting its utility relative to other test types. Researchers have proposed the development of sublethal endpoints for the FET test to increase its utility. The present study 1) developed methods for previously unmeasured sublethal metrics in fathead minnows (i.e., spontaneous contraction frequency and heart rate) and 2) investigated the responsiveness of several sublethal endpoints related to growth (wet weight, length, and growth-related gene expression), neurodevelopment (spontaneous contraction frequency, and neurodevelopmental gene expression), and cardiovascular function and development (pericardial area, eye size and cardiovascular related gene expression) as additional FET test metrics using the model toxicant 3,4 –dichloroaniline. Of the growth, neurological and cardiovascular endpoints measured, length, eye size and pericardial area were found to more responsive than the other endpoints, respectively. Future studies linking alterations in these endpoints to longer-term adverse impacts are needed to fully evaluate the predictive power of these metrics in chemical and whole effluent toxicity testing.
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