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再生水中弗氏柠檬酸杆菌生物膜特性研究

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张静, 李进, 王开强, 孟小然. 再生水中弗氏柠檬酸杆菌生物膜特性研究[J]. 环境化学, 2011, 30(10): 1687-1694.
引用本文: 张静, 李进, 王开强, 孟小然. 再生水中弗氏柠檬酸杆菌生物膜特性研究[J]. 环境化学, 2011, 30(10): 1687-1694.
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ZHANG Jing, LI Jin, WANG Kaiqiang, MENG Xiaoran. STUDY ON THE CHARACTERISTIC OF CITROBACTER FREUNDII BIOFILM IN RECLAIMED WATER[J]. Environmental Chemistry, 2011, 30(10): 1687-1694.
Citation: ZHANG Jing, LI Jin, WANG Kaiqiang, MENG Xiaoran. STUDY ON THE CHARACTERISTIC OF CITROBACTER FREUNDII BIOFILM IN RECLAIMED WATER[J]. Environmental Chemistry, 2011, 30(10): 1687-1694.
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再生水中弗氏柠檬酸杆菌生物膜特性研究

  • 1.

    北京交通大学市政与环境工程系, 北京, 100044

  • 基金项目:

    河北大唐国际张家口热电有限责任公司基金(CX10001)资助.

STUDY ON THE CHARACTERISTIC OF CITROBACTER FREUNDII BIOFILM IN RECLAIMED WATER

  • 1.

    Department of Municipal and Environmental Engineering, Beijing Jiaotong University, Beijing, 100044, China

  • Fund Project:

  • 摘要: 从使用再生水的某电厂冷却塔粘泥中分离出一株具有硫酸盐还原功能的弗氏柠檬酸杆菌.利用扫描电子显微镜(SEM)和能谱仪(EDS)对再生水中HSn70-1A和BFe30-1-1两种合金表面弗氏柠檬酸杆菌生物膜结构和成分进行表征,结果表明HSn70-1A成膜特性好于BFe30-1-1,两种合金表面生物膜中C、O等无机元素含量较大、基体金属元素含量下降较为明显.对不同时刻合金表面生物膜胞外聚合物(EPS)组分进行分析,多糖与蛋白质含量之比揭示出,在第3天和第7天两个关键期EPS的疏水性能对微生物膜的致密性和完整性产生重要影响,实验结果表明在两个关键期内HSn70-1A显示较好的疏水性能,使其比BFe30-1-1表现出较强的耐微生物腐蚀性能.
    Abstract: A strain of Citrobacter freundii with sulfate-reducing function was isolated from the sludge at the bottom of cooling tower in a power plant using reclaimed water. Scanning electron microscopy (SEM) and energy dispersive spectrometry (EDS) were employed to investigate the characteristics of the biofilm formed on the surface of HSn70-1A and BFe30-1-1 in reclaimed water. The results show that the characteristics of biofilm-forming for HSn70-1A was better than BFe30-1-1. In biofilm the contents of C, O and other inorganic elements were relatively high, while the contents of matrix elements significantly decreased. The components of extracellular polymer substances(EPS)in the bio-film formed on the surface of copper alloy at the different time were analyzed. Differences in the ratio of polysaccharide to protein revealed that the 3rd day and 7th day were two critical periods for the formation of biofilm. During these two periods,EPS from HSn70-1A showed better hydrophobic properties than EPS from BFe30-1-1,which made HSn70-1A more resistant to microorganism corrosion.
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  • [1] 孙心利.城市污水再生水回用于电厂循环水的系统腐蚀及防护措施[J]. 中国电力,2007,40(6):28-31
    [2] Zhang Jing,Li Jin,Wu Chuan,et al.Citrobacter freundii on corrosion behavior of HSn70-1A in reclaimed water. The International Conference on E-Product,E-Service and E-Entertainment(ICEEE2010),2010,Vol 8:5309-5314
    [3] Meenakshi Chandel, Wamik Azmi. Optimization of process parameters for the production of tyrosine phenol lyase by Citrobacter freundii MTCC 2424[J]. Bioresource Technology, 2009,100(5): 1840-1846
    [4] Khorasani G, Salehifar E, Eslami G. Profile of microorganisms and antimicrobial resistance at a tertiary care referral burn centre in Iran: Emergence of Citrobacter freundii as a common microorganism[J]. Burns, 2008, 34(7):947-952
    [5] 杨晶. 柠檬酸杆菌吸附重金属铀的试验研究[J].中国给水排水, 2009,25(23):66-68
    [6] Fang Herbert H P, Chan Kwong-Yu, Xua Li-Chong. Quantification of bacterial adhesion forces using atomic force microscopy (AFM)[J].Journal of Methods Microbiological,2000,40(2):89-97
    [7] Postgate J R. The Sulfate Reducing Bacteria [M]. Cambridge: Cambridge University Press, 1984:102-108
    [8] Liu Hong, Herbert H, Fang P. Extraction of extracellular polymeric substances (EPS) of sludges[J].Journal of Biotechnology, 2002,95(3):249-256
    [9] 张龙翔,张庭芳. 生化实验方法和技术[M]. 北京: 高等教育出版社, 2005: 1-3
    [10] 李建武等.生物化学实验原理和方法[M]. 北京: 北京大学出版社, 2000: 174-176
    [11] Evans T E. Chart A, Skedgell A N, et al. The colored film on stainless steel[J]. Trans Inst of Metal Finish, 1972,51(3):108-112
    [12] de Beer Dirk, Stoodley Paul, Roe Frank, et al. Effects of biofilm structures on oxygen distribution and mass transport[J]. Biotechnology and bioengineering, 1994, 43(11):1131-1138
    [13] John A,Nislsen P H. Cell biomass and exopolymer compositions in sewer biofilm[J].Wat Sci Tech,1998,37(1):17-24
    [14] 李久义,左华,栾兆坤,等.不同基质条件对生物膜细胞外聚合物组成和含量的影响[J].环境化学,2002,21(6):546-550
    [15] Wrangstadh M, Conway P L, Kjelleberg S. The production and release of an extracellular polysaccharide during starvation of a marine Pscudomonas sp. and the effect thereof on adhesion[J]. Appl Microbiol, 1986, 145(1/4): 220-227
    [16] Jorand F, Bou-Bigne F, Block J C, et al. Hyrophobic/hydrophilic properties of activated sludge exopolymeric substances[J]. Wat Sci Tech, 1998, 37(4/5): 307-315
    [17] Rosenberg E, Legman R, Kushmaro A, et al. Oil bioremediation using insoluble nitrogen source[J]. Journal of Biotechnology, 1996, 51(3):273-278
    [18] Little B, Wagner P, Mansfeld F. Microbiologically influenced corrosion of metals and alloys[J]. Inter. Materials Reviews, 1991, 36(6): 253
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  • 收稿日期:  2010-12-20

再生水中弗氏柠檬酸杆菌生物膜特性研究

  • 1. 北京交通大学市政与环境工程系, 北京, 100044
  • 收稿日期:  2010-12-20
基金项目:

河北大唐国际张家口热电有限责任公司基金(CX10001)资助.

摘要: 从使用再生水的某电厂冷却塔粘泥中分离出一株具有硫酸盐还原功能的弗氏柠檬酸杆菌.利用扫描电子显微镜(SEM)和能谱仪(EDS)对再生水中HSn70-1A和BFe30-1-1两种合金表面弗氏柠檬酸杆菌生物膜结构和成分进行表征,结果表明HSn70-1A成膜特性好于BFe30-1-1,两种合金表面生物膜中C、O等无机元素含量较大、基体金属元素含量下降较为明显.对不同时刻合金表面生物膜胞外聚合物(EPS)组分进行分析,多糖与蛋白质含量之比揭示出,在第3天和第7天两个关键期EPS的疏水性能对微生物膜的致密性和完整性产生重要影响,实验结果表明在两个关键期内HSn70-1A显示较好的疏水性能,使其比BFe30-1-1表现出较强的耐微生物腐蚀性能.

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