摘要:
多环芳烃(PAHs)对人体健康危害很大,而摄食暴露是PAHs暴露的重要途径之一。PAHs在人体内会代谢成包括羟基多环芳烃(OH-PAHs)在内的一系列代谢产物,并最终随尿液排出体外。为了研究摄入烧烤羊肉导致的PAHs暴露和暴露者尿样中OH-PAHs的含量和动态变化,征集了4名志愿者,在控制条件下一次性定量摄入烧烤羊肉后,测定了羊肉样品中的PAHs含量以及受试者0~36 h期间尿样中的OH-PAHs含量。结果表明,摄入的烧烤羊肉中16种母体PAHs含量为(1 400±95) ng·g-1,其中苯并(a)芘(BaP)为(1.6±2.8) ng·g-1,优势化合物为萘和菲。4位志愿者对16种PAHs和BaP的一次摄入量分别为(330 000±6 900) ng和(370±7.7) ng,显著高于正常饮食条件下的摄入量。在暴露8 h后,2-OH-NAP含量比基线(0.30~0.69μmol·mol-1Cr)升高一个数量级,为2.9~6.3μmol·mol-1Cr,1-OH-PYR也从0.17μmol·mol-1Cr基线含量升至0.40~3.11μmol·mol-1Cr。研究发现,在摄食暴露后,本研究中志愿者(非吸烟者)的尿液中OH-PAHs含量显著高于非职业暴露非吸烟人群的背景水平,但远远低于相关基准值。不同志愿者尿样OH-PAHs含量有明显差别,且与母体PAHs暴露量成正比。根据所测5种OH-PAHs的检出水平及其与母体PAHs的相关关系判断,1-OH-PYR是其中最佳的PAHs暴露指示物。
Abstract:
Polycyclic aromatic hydrocarbons(PAHs) have severely adverse effects on human health,and it is reported that ingestion exposure is one of the main pathways of PAHs exposure.After entering the human body,PAHs can be metabolized to various products,including hydroxy-PAHs(OH-PAHs) that will excrete with urine.To study the intake of PAHs via roast lamb ingestion and the corresponding concentrations of OH-PAHs in urine samples as well as the dynamic change of urinary OH-PAHs,four volunteers were collected to be exposed to PAHs by a single-dose ingestion of roast lamb under controlled conditions.PAHs contents in the meat and OH-PAHs contents in the urine samples during a 36 h period after the exposure were measured.The contents of total PAHs and benzo(a)pyrene(BaP) in the meat were(1400±95) and(1.6±2.8) ng·g-1,respectively,and naphthalene and phenanthrene were the dominant compounds in all tested PAHs.The individual intake of PAHs and BaP were(330 000±6 900) and(370±7.7) ng,respectively,which were significantly higher than usual daily intakes.It is found that at 8 h after the exposure,the urinary content of 2-OH-naphthlene reached 2.9-6.3 μmol·mol-1 Cr,an order of magnitude higher than baseline value of 0.30-0.69 μmol·mol-1 Cr,and the urinary content of 1-OH-pyrene increased from baseline value of 0.17 μmol·mol-1 Cr to 0.40-3.11 μmol·mol-1 Cr.It is demonstrated that the urinary contents of OH-PAHs in non-smoking volunteers in this study after ingestion exposure are significantly higher than the baseline levels of non-smokers without occupational exposure,but are below the criteria value for risk assessment.The urinary OH-PAHs contents correlated well with PAHs intake though the urinary OH-PAHs contents varied among different individuals.1-OH-PYR was found to be the best exposure biomarker among the five tested PAHs in terms of its detectivity and correlation with parent PAHs intake.
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