一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响

吴晓娜, 王助贫, 谢恩, 任梦丽, 刘婷婷, 郑蕾, 丁爱中. 一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响[J]. 环境工程学报, 2018, 12(2): 544-551. doi: 10.12030/j.cjee.201706224
引用本文: 吴晓娜, 王助贫, 谢恩, 任梦丽, 刘婷婷, 郑蕾, 丁爱中. 一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响[J]. 环境工程学报, 2018, 12(2): 544-551. doi: 10.12030/j.cjee.201706224
WU Xiaona, WANG Zhupin, XIE En, REN Mengli, LIU Tingting, ZHENG Lei, DING Aizhong. Screening of one strain of denitrifying phosphorus accumulation bacteria (DPAB) and inhibitions effects of nitrogen-phosphorus removal[J]. Chinese Journal of Environmental Engineering, 2018, 12(2): 544-551. doi: 10.12030/j.cjee.201706224
Citation: WU Xiaona, WANG Zhupin, XIE En, REN Mengli, LIU Tingting, ZHENG Lei, DING Aizhong. Screening of one strain of denitrifying phosphorus accumulation bacteria (DPAB) and inhibitions effects of nitrogen-phosphorus removal[J]. Chinese Journal of Environmental Engineering, 2018, 12(2): 544-551. doi: 10.12030/j.cjee.201706224

一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响

  • 基金项目:

    北京市科技计划项目(D161100000216001)

    国家自然科学基金资助项目(51509003)

Screening of one strain of denitrifying phosphorus accumulation bacteria (DPAB) and inhibitions effects of nitrogen-phosphorus removal

  • Fund Project:
  • 摘要: 以环形推流一体化氧化沟反应器厌氧段污泥为接种污泥,通过吸磷实验、硝酸盐还原产气实验、异染颗粒染色实验及PHB染色实验从接种污泥中筛选得到一株能实现同步脱氮除磷菌株N-12;经过16S rDNA测序鉴定,确定该菌株为不动杆菌属(Acinetobacter)。绘制该菌株的生长曲线,并设置0%、1%、2%、5%、10%和15%等6种不同初始接种比,以接种比作为微生物生物量的衡量参数,研究不同接种比对反硝化聚磷菌脱氮除磷效应的影响,确定菌株最佳接种比。实验结果表明:初始接种比2%体系的脱氮除磷效果最好;利用SPSS statistics 19统计软件对磷酸盐去除率、硝态氮去除率、OD600值及pH进行相关性分析,表明磷酸盐去除率和硝酸盐去除率显著正相关(Person相关系数=0.826,P600值及pH值都显著正相关。
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  • 刊出日期:  2018-02-08
吴晓娜, 王助贫, 谢恩, 任梦丽, 刘婷婷, 郑蕾, 丁爱中. 一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响[J]. 环境工程学报, 2018, 12(2): 544-551. doi: 10.12030/j.cjee.201706224
引用本文: 吴晓娜, 王助贫, 谢恩, 任梦丽, 刘婷婷, 郑蕾, 丁爱中. 一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响[J]. 环境工程学报, 2018, 12(2): 544-551. doi: 10.12030/j.cjee.201706224
WU Xiaona, WANG Zhupin, XIE En, REN Mengli, LIU Tingting, ZHENG Lei, DING Aizhong. Screening of one strain of denitrifying phosphorus accumulation bacteria (DPAB) and inhibitions effects of nitrogen-phosphorus removal[J]. Chinese Journal of Environmental Engineering, 2018, 12(2): 544-551. doi: 10.12030/j.cjee.201706224
Citation: WU Xiaona, WANG Zhupin, XIE En, REN Mengli, LIU Tingting, ZHENG Lei, DING Aizhong. Screening of one strain of denitrifying phosphorus accumulation bacteria (DPAB) and inhibitions effects of nitrogen-phosphorus removal[J]. Chinese Journal of Environmental Engineering, 2018, 12(2): 544-551. doi: 10.12030/j.cjee.201706224

一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响

  • 1. 北京师范大学水科学研究院,北京100875
  • 2. 北控水务集团有限公司,北京 100102
  • 3. 中国农业大学水利与土木工程学院,北京 100083
基金项目:

北京市科技计划项目(D161100000216001)

国家自然科学基金资助项目(51509003)

摘要: 以环形推流一体化氧化沟反应器厌氧段污泥为接种污泥,通过吸磷实验、硝酸盐还原产气实验、异染颗粒染色实验及PHB染色实验从接种污泥中筛选得到一株能实现同步脱氮除磷菌株N-12;经过16S rDNA测序鉴定,确定该菌株为不动杆菌属(Acinetobacter)。绘制该菌株的生长曲线,并设置0%、1%、2%、5%、10%和15%等6种不同初始接种比,以接种比作为微生物生物量的衡量参数,研究不同接种比对反硝化聚磷菌脱氮除磷效应的影响,确定菌株最佳接种比。实验结果表明:初始接种比2%体系的脱氮除磷效果最好;利用SPSS statistics 19统计软件对磷酸盐去除率、硝态氮去除率、OD600值及pH进行相关性分析,表明磷酸盐去除率和硝酸盐去除率显著正相关(Person相关系数=0.826,P600值及pH值都显著正相关。

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