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Trametes sp.LS-10C漆酶对直接类偶氮染料的脱色作用

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李松, 刘宇, 海丹丹, 陈阿娜, 汤文晶, 汤斌. Trametes sp.LS-10C漆酶对直接类偶氮染料的脱色作用[J]. 环境工程学报, 2016, 10(10): 6071-6076. doi: 10.12030/j.cjee.201505154
引用本文: 李松, 刘宇, 海丹丹, 陈阿娜, 汤文晶, 汤斌. Trametes sp.LS-10C漆酶对直接类偶氮染料的脱色作用[J]. 环境工程学报, 2016, 10(10): 6071-6076. doi: 10.12030/j.cjee.201505154
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LI Song, LIU Yu, HAI Dandan, CHEN Ana, TANG Wenjing, TANG Bin. Decolorization of direct azo dyes by laccase from Trametes sp.LS-10C[J]. Chinese Journal of Environmental Engineering, 2016, 10(10): 6071-6076. doi: 10.12030/j.cjee.201505154
Citation: LI Song, LIU Yu, HAI Dandan, CHEN Ana, TANG Wenjing, TANG Bin. Decolorization of direct azo dyes by laccase from Trametes sp.LS-10C[J]. Chinese Journal of Environmental Engineering, 2016, 10(10): 6071-6076. doi: 10.12030/j.cjee.201505154
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Trametes sp.LS-10C漆酶对直接类偶氮染料的脱色作用

  • 1.

    安徽工程大学生物与化学工程学院, 芜湖 241000

  • 2.

    微生物发酵安徽省工程技术研究中心, 芜湖 241000

  • 基金项目:

    安徽省自然科学青年基金资助项目（1408085QC61）

    安徽工程大学引进人才科研启动基金（2013YQQ003）

  • 中图分类号: X172

Decolorization of direct azo dyes by laccase from Trametes sp.LS-10C

  • 1.

    School of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu 241000, China

  • 2.

    Microorganism Fermentation Engineering and Technology Research Center, Wuhu 241000, China

  • Fund Project:

  • 摘要: 利用栓菌（Trametes sp.LS-10C）漆酶在单酶和漆酶-介体体系2种情况下对3种直接类偶氮染料进行脱色。结果表明，在给酶量为10 U·mL-1、染料浓度为100 mg·mL-1条件下反应24 h后，直接蓝86在非介体体系中脱色率达到71.2%，其最适脱色温度和pH范围分别为45~60℃和3.5~5.0；直接橙26在介体体系（10 μmol·L-1 ABTS）中脱色率达到78.7%，其最适脱色温度和pH范围分别为35~45℃和4.5~6.5；直接红31在介体体系（10 μmol·L-1 ABTS）中脱色率达到90.7%，其最适脱色温度和pH范围分别为35~45℃和3.5~5.0。
    Abstract: Laccase from Trametes sp.LS-10C was used for decolorization of three kinds of direct azo dyes in both single enzyme (non-mediator) and enzyme-mediator systems.The results showed that for 10 U·mL-1 enzyme,the dye concentration was 100 mg·mL-1.After 24 h incubation,the decolorization rate of Direct Blue 86 in a non-mediator system reached 71.2%,with optimum decolorization temperature and pH of 45 to 60℃ and 3.5 to 5.0,respectively.Similarly,the decolorization rate of Direct Orange 26 in a mediator system (10 μmol·L-1 ABTS) reached 78.7%,with optimum decolorization temperature and pH of 35 to 45℃ and 4.5 to 6.5,respectively,and the decolorization rate of Direct Red 31 in a mediator system (10 μmol·L-1 ABTS) reached 90.7%,with optimum decolorization temperature and pH of 35 to 45℃ and 3.5 to 5.0,respectively.
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  • 收稿日期:  2015-05-28
  • 刊出日期:  2016-10-20

Trametes sp.LS-10C漆酶对直接类偶氮染料的脱色作用

  • 1.  安徽工程大学生物与化学工程学院, 芜湖 241000
  • 2.  微生物发酵安徽省工程技术研究中心, 芜湖 241000
  • 收稿日期:  2015-05-28
基金项目:

安徽省自然科学青年基金资助项目（1408085QC61）

安徽工程大学引进人才科研启动基金（2013YQQ003）

摘要: 利用栓菌（Trametes sp.LS-10C）漆酶在单酶和漆酶-介体体系2种情况下对3种直接类偶氮染料进行脱色。结果表明，在给酶量为10 U·mL-1、染料浓度为100 mg·mL-1条件下反应24 h后，直接蓝86在非介体体系中脱色率达到71.2%，其最适脱色温度和pH范围分别为45~60℃和3.5~5.0；直接橙26在介体体系（10 μmol·L-1 ABTS）中脱色率达到78.7%，其最适脱色温度和pH范围分别为35~45℃和4.5~6.5；直接红31在介体体系（10 μmol·L-1 ABTS）中脱色率达到90.7%，其最适脱色温度和pH范围分别为35~45℃和3.5~5.0。

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