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周月明, 刘娜, 李斗, 尹雅洁, 宋立岩, 张雷. 苯系物降解单菌间的协同耦合作用[J]. 环境工程学报, 2014, 8(11): 5049-5056.
引用本文: 周月明, 刘娜, 李斗, 尹雅洁, 宋立岩, 张雷. 苯系物降解单菌间的协同耦合作用[J]. 环境工程学报, 2014, 8(11): 5049-5056.
shu
Zhou Yueming, Liu Na, Li Dou, Yin Yajie, Song Liyan, Zhang Lei. Synergetic and coupled effects of bacteria in a BTEX degrading community[J]. Chinese Journal of Environmental Engineering, 2014, 8(11): 5049-5056.
Citation: Zhou Yueming, Liu Na, Li Dou, Yin Yajie, Song Liyan, Zhang Lei. Synergetic and coupled effects of bacteria in a BTEX degrading community[J]. Chinese Journal of Environmental Engineering, 2014, 8(11): 5049-5056.
shu

苯系物降解单菌间的协同耦合作用

  • 1.

    中国科学院重庆绿色智能技术研究院, 重庆 400714

  • 2.

    吉林大学环境与资源学院, 长春 130026

  • 基金项目:

    中国科学院西部行动计划项目(KZCX2-XB3-14)

    2012年中国科学院"西部之光"人才培养计划西部博士资助项目

  • 中图分类号: X172

Synergetic and coupled effects of bacteria in a BTEX degrading community

  • 1.

    Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China

  • 2.

    College of Environment and Resources, Jilin University, Changchun 130026, China

  • Fund Project:

摘要

  • 摘要: 针对BTEX降解混菌间协同耦合作用不明的问题,以稳定的BTEX降解混菌为研究对象,通过分离纯化,从混菌中分离得到的8株BTEX降解单菌,将对BTEX有降解效果和没有降解效果的单菌分别组合,采用摇瓶实验研究不同体系对BTEX的降解效果,试图找到单菌在BTEX降解过程中的作用,并探寻菌间的协同耦合作用.实验结果表明,B2、B4、B6单菌主要的作用是降解BTEX,并且B2菌可以解除B4菌和B6菌混合后对X降解的抑制,并提高了B、T和E的降解速率;B1、B3、B5、B7和B8单菌无BTEX降解功能,B1、B3和B5菌可能利用B、T和E降解过程中产生的代谢产物(各单菌没有降解能力,但在混菌中依然存在,推测单菌可以利用降解过程中产生的代谢产物),并从48 h开始降解B、T和E;B7、B8两株菌互相协同,共同完成对BTEX的降解.由优势菌群和非优势菌群组成的混合系统,促使有些没有降解功能的菌株混合后具有降解功能,还导致有些菌株混合后降解率和降解速率降低,对降解BTEX既有促进作用又有拮抗作用.混菌48 h对BTEX的总降解率为91.6%,说明一个稳定存在的天然混合群落,其内部菌种经过长期的优胜劣汰,保持了很高的降解率和降解速率.
    Abstract: Synergetic and coupled effects are common strategy for microorganism to utilize BETX, however, the effects mechanism between bacteria remain largely unknown. To test the mechanism, we analyzed the BETX degradation behavior by single and combined strains isolated from a stable BTEX-degradation microflora enriched from BTEX-contaminated soil. In briefly, eight strains, referred to as B1, B2, B3, B4, B5, B6, B7, and B8 were isolated from the microflora and divided into groups according to their degradation ability. One group decomposes BTEX, consisting of B2, B4, and B6. In contract, the rest isolates could not use BTEX. The BTEX-degradation test showed that the combined culture of B4 and B6 suppresses X-degradation, but B2 could release the suppression and subsequently increase the utilization rate of B, T, E. Although B1, B3, B5, B7, and B8 could not use BETX, B1, B3, and B5 might use the metabolite of B, T, E-degradation because they decomposes B, T, E after 48 hours. The mixed culture of B7 and B8 is able to degrade BTEX. In addition, the stable BTEX-degradation microflora reduced 91.6% BETX within 48 hours, higher than any single or combinations. Overall, the findings provide the direct evidence that the synergetic and coupled effects of bacteria highly impact on BETX biodegradation.
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出版历程
  • 收稿日期:  2013-10-14
  • 刊出日期:  2014-11-07
周月明, 刘娜, 李斗, 尹雅洁, 宋立岩, 张雷. 苯系物降解单菌间的协同耦合作用[J]. 环境工程学报, 2014, 8(11): 5049-5056.
引用本文: 周月明, 刘娜, 李斗, 尹雅洁, 宋立岩, 张雷. 苯系物降解单菌间的协同耦合作用[J]. 环境工程学报, 2014, 8(11): 5049-5056.
shu
Zhou Yueming, Liu Na, Li Dou, Yin Yajie, Song Liyan, Zhang Lei. Synergetic and coupled effects of bacteria in a BTEX degrading community[J]. Chinese Journal of Environmental Engineering, 2014, 8(11): 5049-5056.
Citation: Zhou Yueming, Liu Na, Li Dou, Yin Yajie, Song Liyan, Zhang Lei. Synergetic and coupled effects of bacteria in a BTEX degrading community[J]. Chinese Journal of Environmental Engineering, 2014, 8(11): 5049-5056.
shu

苯系物降解单菌间的协同耦合作用

  • 1. 中国科学院重庆绿色智能技术研究院, 重庆 400714
  • 2. 吉林大学环境与资源学院, 长春 130026
  • 收稿日期:  2013-10-14
基金项目:

中国科学院西部行动计划项目(KZCX2-XB3-14)

2012年中国科学院"西部之光"人才培养计划西部博士资助项目

摘要: 针对BTEX降解混菌间协同耦合作用不明的问题,以稳定的BTEX降解混菌为研究对象,通过分离纯化,从混菌中分离得到的8株BTEX降解单菌,将对BTEX有降解效果和没有降解效果的单菌分别组合,采用摇瓶实验研究不同体系对BTEX的降解效果,试图找到单菌在BTEX降解过程中的作用,并探寻菌间的协同耦合作用.实验结果表明,B2、B4、B6单菌主要的作用是降解BTEX,并且B2菌可以解除B4菌和B6菌混合后对X降解的抑制,并提高了B、T和E的降解速率;B1、B3、B5、B7和B8单菌无BTEX降解功能,B1、B3和B5菌可能利用B、T和E降解过程中产生的代谢产物(各单菌没有降解能力,但在混菌中依然存在,推测单菌可以利用降解过程中产生的代谢产物),并从48 h开始降解B、T和E;B7、B8两株菌互相协同,共同完成对BTEX的降解.由优势菌群和非优势菌群组成的混合系统,促使有些没有降解功能的菌株混合后具有降解功能,还导致有些菌株混合后降解率和降解速率降低,对降解BTEX既有促进作用又有拮抗作用.混菌48 h对BTEX的总降解率为91.6%,说明一个稳定存在的天然混合群落,其内部菌种经过长期的优胜劣汰,保持了很高的降解率和降解速率.

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