牛粪堆肥升温菌剂的筛选及其作用机制

方华舟, 左雪枝. 牛粪堆肥升温菌剂的筛选及其作用机制[J]. 环境工程学报, 2013, 7(9): 3689-3694.
引用本文: 方华舟, 左雪枝. 牛粪堆肥升温菌剂的筛选及其作用机制[J]. 环境工程学报, 2013, 7(9): 3689-3694.
Fang Huazhou, Zuo Xuezhi. Isolation of heating-up microorganisms from cow dung compost and their mechanism of action[J]. Chinese Journal of Environmental Engineering, 2013, 7(9): 3689-3694.
Citation: Fang Huazhou, Zuo Xuezhi. Isolation of heating-up microorganisms from cow dung compost and their mechanism of action[J]. Chinese Journal of Environmental Engineering, 2013, 7(9): 3689-3694.

牛粪堆肥升温菌剂的筛选及其作用机制

  • 基金项目:

    湖北省自然科学基金项目(2010-97-299)

  • 中图分类号: X172;S141.4

Isolation of heating-up microorganisms from cow dung compost and their mechanism of action

  • Fund Project:
  • 摘要: 以糖、淀粉、蛋白质、纤维素培养基及菌株增殖倍数、菌株性能等为筛选方法,从牛粪低温堆肥中筛选不同原料重要功能菌并组成功能菌剂;以不同类别原料菌及单菌株发酵实验对升温机制进行研究。结果表明,芽孢杆菌、假单胞菌、纤维单胞菌及小孢霉菌等为重要功能菌;菌剂起温快,升温效果好,48 h堆体即由低温进入中温,5 d达到高温,腐熟时间可缩短至约17~18 d;糖功能菌升温效果最为显著,淀粉及蛋白质功能菌次之,纤维素功能菌升温能力较弱;菌株利用各种原料能力越强,增殖速度越快,对升温作用越大。各菌株充分利用基质各成分,紧密协同和促进,是低温堆体发酵升温的主要作用机制。
  • [1] 陈素华,孙铁衍,耿春女.我国禽畜养殖业引致的环境问题及主要对策.环境污染治理技术与设备,2003,4(5):5-8 ChenSuhua,SunTieheng,Geng Chunnu.Environmental pollution caused by livestock husbandry and its control measures.Techniques and Equipment for Environmental Pollution Control,2003,4(5):5-8(in Chinese)
    [2] Raut M. P.,Prince William S. P. M.,Bhattacharyya J. K.,et al.Microbial dynamics and enzyme activities during rapid composting of minicipal solid waste: A compost maturity analysis perspective .Bioresource Technology, 2008,99(14):6512-6519
    [3] 隋文志,胡广民,赵晓锋,等.北方寒区牛粪无害化处理关键技术研究.农业环境科学学报, 2010,29(3):578-585 Sui Wenzhi,Hu Guangmin,Zhao Xiaofeng,et al.Key technology of caw dung composting innocuous in northern cold regions.Journal of Agro-Environment Science, 2010,29(3):578-585(in Chinese)
    [4] 何惠霞,徐凤花,赵晓锋,等.低温下牛粪接种发酵剂对堆肥温度与微生物的影响.东北农业大学学报,2007,38(1):54-58 He Huixia,Xu Fenghua,Zhao Xiaofeng,et al.Effects of inoculated complex microbial agent to cattle manure on compost temperature and microorganism under low temperature.Journal of Northeast Agricultural University,2007,38(1):54-58(in Chinese)
    [5] 李国华,张福锁.固体废弃物堆肥化与有机复混肥生产.北京:化学工业出版社,2000
    [6] 李玉华.有机肥生产与应用.天津:天津科技翻译出版公司,2010
    [7] 牛俊玲.多功能堆肥复合菌剂.北京:化学工业出版社,2009
    [8] Haug R. T.The Practical Handbook of Composting Engineering.BoCa Roton: Lewis Publishers,1993
    [9] 刁春霞,张雪梅,刘波.干红葡萄酒中总糖测定方法的改进.中国酿造, 2009,28(5):165-166 Diao Chunxia,Zhang Xuemei,Liu Bo.Improvement of determination method of the total sugar in dry red wine.China Brewing, 2009,28(5):165-166(in Chinese)
    [10] 丁映,陈鹰,乐俊明,等.利用酸水解法测定烟叶中淀粉的含量.贵州农业科学,2004,32(6):72 Ding Ying,Cheng Ying,Yue Junming,et al.Starch determination in tobacco by using acid-hydrolysis.Guizhou Agricultural Sciences, 2004,32(6):72(in Chinese)
    [11] 刘燕,张平,倪艳,等.双缩脲法测定大豆乳清废水中蛋白质含量.大豆通报,2006,(6):24-26 Liu Yan,Zhang Ping,Ni Yan,et al.Biuret method to measure the protein content in soybean whey.Soybean Bulletin,2006,(6):24-26(in Chinese)
    [12] 布坎南R.E.,李本斯N.E. 伯杰细菌鉴定手册.北京:科学出版社,1984
    [13] 魏景超.真菌鉴定手册.上海:上海科学出版社,1979
    [14] 王伟东,刘建斌,牛俊玲,等.堆肥化过程中微生物群落的动态及接菌剂的应用效果.农业工程学报,2006,22(4):148-152 Wang Weidong,Liu Jianbing,Niu Junling,et al.Dynamics of microbial community and the effect of inoculum on the composting.Transactions of the Chinese Society of Agricultural Engineering,2006,22(4):148-152(in Chinese)
    [15] 石铁,盛力伟,闫景凤.低温下菌剂对堆肥温度与微生物的影响.农机化研究, 2008,(1):120-122 Shi Tie,Sheng Liwei,Yan Jingfeng.The Impact of low temperatures bacteria for compost temperature and microorganism.Journal of Agricultural Mechanization Research, 2008,(1):120-122(in Chinese)
    [16] 孙俊丽,王顺利,刘克锋,等.牛粪堆肥高温发酵微生物分离及堆肥效果.安徽农业科学, 2010,38(35):20057-20059,20071 Sun Junli,Wang Shunli,Liu Kefeng,et al.Isolation of thermophile microorganism from cow dung and effects on cow dung composting.Journal of Anhui Agricultural Sciences, 2010,38(35):20057-20059,20071(in Chinese)
    [17] Kato K.,Miutra N.Effent of matured compost as a bulking and inoeulating agent on the mierobial community and maturity of cattle manure compost.Bioresource Technology, 2008,99(9):3372-3380
    [18] 黄得扬,陆文静.有机固体废弃物堆肥化处理的微生物学机理研究进展.环境污染治理技术与设备,2004,5(1):12-17 Huang Deyang,Lu Wenjing,Wang Hongtao.Microbiological mechanismof organic solid wastes composting.Technigues and Equipment For Environmental Pollution Control,2004,5(1):12-17(in Chinese)
    [19] 熊冬梅,周红丽.纤维素降解菌群的研究进展.酿酒科技, 2011,(5):94-97 Xiong Dongmei,Zhou Hongli.Research progress in cellulose-degrading bacteria community.Liquor-Making Science & Technology, 2011,(5):94-97(in Chinese)
    [20] 周晓红,陈洪章,李佐虎.固态发酵中纤维素基质降解过程初步研究.过程工程学报,2003,3(5):447-452 Zhou Xiaohong,Chen Hongzhang,Li Zuohu.Experimental observation on cellulosic biodegradation in solid state fermentation.The Chinese Journal of Process Engineering,2003,3(5):447-452(in Chinese)
    [21] 牛俊玲,崔宗均,李国学,等.高温堆肥中复合菌系对木质纤维素和林丹降解效果的研究.农业环境科学学报,2005,24(2):375-379 Niu Junling,Cui Zongjun,Li Guoxue,et al.Degradation of lignocellulose and lindane in microbial composting system during thermophilic compost process. Journal of Agro-environmental Science,2005,24(2):375-379(in Chinese)
    [22] Geeraerts H. A. M.,Vandamme E. J.Cellulolytic properties of choetomiun crispatum.Journal of Chemical Technology and Biotechnology, 2008,33(2):107-113
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出版历程
  • 收稿日期:  2012-06-29
  • 刊出日期:  2013-09-15
方华舟, 左雪枝. 牛粪堆肥升温菌剂的筛选及其作用机制[J]. 环境工程学报, 2013, 7(9): 3689-3694.
引用本文: 方华舟, 左雪枝. 牛粪堆肥升温菌剂的筛选及其作用机制[J]. 环境工程学报, 2013, 7(9): 3689-3694.
Fang Huazhou, Zuo Xuezhi. Isolation of heating-up microorganisms from cow dung compost and their mechanism of action[J]. Chinese Journal of Environmental Engineering, 2013, 7(9): 3689-3694.
Citation: Fang Huazhou, Zuo Xuezhi. Isolation of heating-up microorganisms from cow dung compost and their mechanism of action[J]. Chinese Journal of Environmental Engineering, 2013, 7(9): 3689-3694.

牛粪堆肥升温菌剂的筛选及其作用机制

  • 1. 荆楚理工学院生物工程学院, 荆门 448000
基金项目:

湖北省自然科学基金项目(2010-97-299)

摘要: 以糖、淀粉、蛋白质、纤维素培养基及菌株增殖倍数、菌株性能等为筛选方法,从牛粪低温堆肥中筛选不同原料重要功能菌并组成功能菌剂;以不同类别原料菌及单菌株发酵实验对升温机制进行研究。结果表明,芽孢杆菌、假单胞菌、纤维单胞菌及小孢霉菌等为重要功能菌;菌剂起温快,升温效果好,48 h堆体即由低温进入中温,5 d达到高温,腐熟时间可缩短至约17~18 d;糖功能菌升温效果最为显著,淀粉及蛋白质功能菌次之,纤维素功能菌升温能力较弱;菌株利用各种原料能力越强,增殖速度越快,对升温作用越大。各菌株充分利用基质各成分,紧密协同和促进,是低温堆体发酵升温的主要作用机制。

English Abstract

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