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有机污染胁迫对人工湿地中浮萍抗氧化系统的影响

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刘娥, 张建, 魏榕, 徐景涛, 闫玉洁, 张波. 有机污染胁迫对人工湿地中浮萍抗氧化系统的影响[J]. 环境工程学报, 2013, 7(9): 3296-3300.
引用本文: 刘娥, 张建, 魏榕, 徐景涛, 闫玉洁, 张波. 有机污染胁迫对人工湿地中浮萍抗氧化系统的影响[J]. 环境工程学报, 2013, 7(9): 3296-3300.
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Liu E, Zhang Jian, Wei Rong, Xu Jingtao, Yan Yujie, Zhang Bo. Effect of organic pollutants stresses on antioxidant defense system of duckweed(Lemna minor)[J]. Chinese Journal of Environmental Engineering, 2013, 7(9): 3296-3300.
Citation: Liu E, Zhang Jian, Wei Rong, Xu Jingtao, Yan Yujie, Zhang Bo. Effect of organic pollutants stresses on antioxidant defense system of duckweed(Lemna minor)[J]. Chinese Journal of Environmental Engineering, 2013, 7(9): 3296-3300.
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有机污染胁迫对人工湿地中浮萍抗氧化系统的影响

  • 1.

    山东大学环境科学与工程学院, 济南 250100

  • 2.

    北京北林地景园林规划设计院有限责任公司, 北京 100083

  • 基金项目:

    国家"水体污染控制与治理"科技重大专项(2012ZX07203-004)

  • 中图分类号: X52

Effect of organic pollutants stresses on antioxidant defense system of duckweed(Lemna minor)

  • 1.

    School of Environmental Science and Engineering, Shandong University, Jinan 250100, China

  • 2.

    Beijing Beilin Landscape Architecture Institute Co. Ltd., Beijing 100083, China

  • Fund Project:

  • 摘要: 湿地植物在处理高负荷有机废水时会受到不同程度的氧化胁迫。本研究基于对浮萍的有机污染胁迫模拟系统,通过对浮萍脂质过氧化和抗氧化防御系统的监测与分析,研究了浮萍对有机污染胁迫的耐受能力及胁迫去除后浮萍的恢复规律。结果表明,浮萍对有机污染胁迫具有较高的耐受性,在胁迫去除后,具有一定的恢复能力。在COD小于400 mg/L时,浮萍并未受到氧化胁迫;当COD达到800 mg/L时,浮萍体内ROS含量上升,细胞膜脂过氧化加剧,但抗氧化酶活性升高,抗氧化物质含量增加,浮萍可保持生长,胁迫去除后,抗氧化防御系统可恢复到对照水平;当COD过高(≥1 000 mg/L),ROS急剧上升,抗氧化防御系统遭受破坏,造成不可逆伤害,胁迫去除后不能恢复正常生长。
    Abstract: Wetland plants maybe suffered from the stress of oxidation when treating with high organic load wastewater. In the present study, we investigated the tolerance ability of the duckweed(Lemna minor)to the organic pollutants stresses and the law of recovery after removing the stresses through analyzing the organic pollutants stresses. Results showed that the duckweed had high tolerance to organic pollutants stresses and had a certain ability to recover after the stresses. When COD was less than 400 mg/L, the duckweeds were not suffered from the oxidation stresses. When COD reached 800 mg/L, the content of ROS increased and the membrane lipid peroxidation aggravated. But the increasing of the antioxidant enzymes activity and the antioxidant content ensured the duckweeds grow well. And the antioxidant defense system could restore to the control level after removing the stresses. When COD was too high(≥1 000 mg/L), the ROS content increased sharply and the antioxidant enzymes system was destroyed. The irreversible damage was caused and the duckweeds could not restore to normal even after removing the stresses.
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  • [1] Kadlec H.R., Knight R.L. Treatment Wetlands. Boca Raton: Lewis Publishers, 1996
    [2] Scholz M. Wetland Systems to Control Urban Runoff. Amsterdam, the Netherlands: Elsevier Science Ltd., 2006
    [3] 范媛媛,袁妙淼,邓梅峰,等. 高浓度氮、磷胁迫对伊乐藻SOD、POD和CAT活性的影响. 氨基酸和生物资源,2007,29(3): 38-41
    [4] J. Xu, C. Li, F. Yang, Z Dong, et al. Typha angustifolia stress tolerance to wastewater with different levels of chemical oxygen demand. Desalination, 2011,280(1-3): 58-62
    [5] J. Xu, J. Zhang, H. Xie, et al. Physiological responses of Phragmites australis to wastewater with different chemical oxygen demands. Ecol. Eng., 2010,36(10): 1341-1347
    [6] 种云霄,胡洪营,钱易. pH及无机氮化合物对小浮萍生长的影响.环境科学, 2003,24(4): 35-40 Chong Yunxiao, Hu Hongying, Qian Yi. Effect of inorganic nitrogen compounds and pH on the growth of duckweed. Environmental Science,2003,24(4): 35-40(in Chinese)
    [7] 王宝山. 植物生理学. 北京: 科学出版社, 2003
    [8] Aebi H. Catalase in Methods of Enzymatic Analysis. N.Y.: Academic Press, 1974. 673-684
    [9] Beauchamp C., Fridovich I. Superoxide dismutase: Improved assays and an assay applicable to acrlamide gels. Ana1. Bichem., 1971,44(1): 276-287
    [10] Kraus T., Fletcher R. Paclobutrazol protects wheat seedings from heat and paraquat injury. Is detoxification of active oxygen involved. Plant Cell Physiol, 1994,35(1): 45-52
    [11] Heath R. L., Packer L. Photoperoxidation in isolated chloroplasts:1.Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem.Biophys.,1968,125(1):189-198
    [12] Anderson M.E. Determination of glutathione and glutathioine disulphide in biological samples. Methods.Enzymol., 1985,113(1): 548-555
    [13] Jana S., Choudhuri M. A. Glycolate metabolism of three submerged aquatic angiosperms during ageing. Aquat.Bot., 1982,12: 345-354
    [14] Bergmann B. A., Cheng J., Classen J., et al. In vitro selection of duckweed geographical isolates for potential use in swine lagoon effluent renovation. Bioresour. Technol., 2000, 73(1): 13-20
    [15] 国家环境保护总局. 水和废水监测分析方法(第4版). 北京: 中国环境科学出版社, 2002
    [16] Mittler R., Vanderauwera S., Gollery M., et al.Reactive oxygen gene network of plants. Trends.Plant. Sci., 2004,9(10): 490-498
    [17] 李合生.现代植物生理学.北京: 高等教育出版社, 2001
    [18] 徐楠,施国新,曾晓敏,等. Hg2+胁迫对浮萍体细胞DNA一级结构和抗氧化酶系的损伤.植物生态学报, 2003,27(3): 299-303 Xu Nan, Shi Guoxin, Zeng Xiaomin,et al. Damage to DNA primary structure and antioxidant enzymes in Lemna Minor induced by Hg2+. Journal of Plant Ecological, 2003,27(3):299-303(in Chinese)
    [19] 尹永强,胡建斌,邓建军.植物叶片抗氧化系统及其对逆境胁迫的响应研究进展.中国农学通报, 2007,23(1): 105-110 Yin Yongqiang,Hu Jianbin, Deng Mingjun. Latest development of antioxidantsystem and responses to stress in plant leaves. Journal of Chinese Agriculture, 2007,23(1): 105-110(in Chinese)
    [20] 王爱国,邵从本,罗广华.丙二醛作为植物脂质过氧化指标的探讨.植物学生理学通讯, 1986, (2): 55-57 Wang Aiguo, Shao Congben, Luo Guanghua. Inquiry into malondialdehyde as index of peroxidation of plant lipids. Plant Physiology Communnications, 1986, (2): 55-57 (in Chinese)
    [21] Chcn S. Y. Injury of membrane lipid peroxidation to plant cell. Plant Physiology Communicstion, 1991,27(2): 84-90
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  • 收稿日期:  2012-07-03
  • 刊出日期:  2013-09-15

有机污染胁迫对人工湿地中浮萍抗氧化系统的影响

  • 1. 山东大学环境科学与工程学院, 济南 250100
  • 2. 北京北林地景园林规划设计院有限责任公司, 北京 100083
  • 收稿日期:  2012-07-03
基金项目:

国家"水体污染控制与治理"科技重大专项(2012ZX07203-004)

摘要: 湿地植物在处理高负荷有机废水时会受到不同程度的氧化胁迫。本研究基于对浮萍的有机污染胁迫模拟系统,通过对浮萍脂质过氧化和抗氧化防御系统的监测与分析,研究了浮萍对有机污染胁迫的耐受能力及胁迫去除后浮萍的恢复规律。结果表明,浮萍对有机污染胁迫具有较高的耐受性,在胁迫去除后,具有一定的恢复能力。在COD小于400 mg/L时,浮萍并未受到氧化胁迫;当COD达到800 mg/L时,浮萍体内ROS含量上升,细胞膜脂过氧化加剧,但抗氧化酶活性升高,抗氧化物质含量增加,浮萍可保持生长,胁迫去除后,抗氧化防御系统可恢复到对照水平;当COD过高(≥1 000 mg/L),ROS急剧上升,抗氧化防御系统遭受破坏,造成不可逆伤害,胁迫去除后不能恢复正常生长。

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