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一株荧蒽降解菌的筛选鉴定及降解特性

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周惠娟, 马艳玲. 一株荧蒽降解菌的筛选鉴定及降解特性[J]. 环境工程学报, 2013, 7(5): 1984-1988.
引用本文: 周惠娟, 马艳玲. 一株荧蒽降解菌的筛选鉴定及降解特性[J]. 环境工程学报, 2013, 7(5): 1984-1988.
shu
Zhou Huijuan, Ma Yanling. Isolation and characteristics of a fluoranthene degrading bacterial strain[J]. Chinese Journal of Environmental Engineering, 2013, 7(5): 1984-1988.
Citation: Zhou Huijuan, Ma Yanling. Isolation and characteristics of a fluoranthene degrading bacterial strain[J]. Chinese Journal of Environmental Engineering, 2013, 7(5): 1984-1988.
shu

一株荧蒽降解菌的筛选鉴定及降解特性

  • 1.

    西部资源与现代生物技术省部共建教育部重点实验室,西北大学生命科学学院,西安 710069

  • 基金项目:

    国家自然科学基金资助项目(31000069)

    陕西省自然科学基金资助项目(2010JM3012)

    陕西省教育厅专项科研计划项目(2010JK853)

  • 中图分类号: X172

Isolation and characteristics of a fluoranthene degrading bacterial strain

  • 1.

    Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi'an 710069, China

  • Fund Project:

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  • 摘要: 采用富集培养方法,从多环芳烃污染的土壤中分离筛选到一株能以高分子量的多环芳烃荧蒽为惟一碳源和能源且生长状况良好的菌株DN002。通过形态观察、生理生化指标及16S rRNA同源序列分析比对,结果表明,该菌株为木糖氧化产碱菌(Achromobacter xylosoxidans),最适生长温度为32℃,最适生长pH为7～7.5。该菌株对荧蒽有较强的降解能力,在14 d内对500 mg/L的荧蒽的降解率为92.8%。菌株细胞蛋白SDS-PAGE结果显示,经荧蒽诱导后,在分子量为18～66 kDa范围内有显著的差异蛋白条带。
    Abstract: A bacterial strain, which utilizied fluoranthene as the sole carbon and energy source for growth, was isolated from soil heavily contaminated by polycyclic aromatic hydrocarbons, designated DN002. This strain was identified as Achromobacter xylosoxidans by its morphology, physiological and biochemical characteristics and 16S rRNA sequence. The optimum growth temperature was 32℃ and the pH was from 7 to 7.5.When the strain was grown in the 500 mg/L fluoranthene for 14 days, the degradation rate was 92.8%. The SDS-PAGE analysis of whole cell protein of the strain after growing with fluoranthene showed that there were appeared distinctive protein brands with molecular weight between 18 kDa and 66 kDa difference to the brands growing without fluoranthene.
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  • 收稿日期:  2012-03-15
  • 刊出日期:  2013-05-22
周惠娟, 马艳玲. 一株荧蒽降解菌的筛选鉴定及降解特性[J]. 环境工程学报, 2013, 7(5): 1984-1988.
引用本文: 周惠娟, 马艳玲. 一株荧蒽降解菌的筛选鉴定及降解特性[J]. 环境工程学报, 2013, 7(5): 1984-1988.
shu
Zhou Huijuan, Ma Yanling. Isolation and characteristics of a fluoranthene degrading bacterial strain[J]. Chinese Journal of Environmental Engineering, 2013, 7(5): 1984-1988.
Citation: Zhou Huijuan, Ma Yanling. Isolation and characteristics of a fluoranthene degrading bacterial strain[J]. Chinese Journal of Environmental Engineering, 2013, 7(5): 1984-1988.
shu

一株荧蒽降解菌的筛选鉴定及降解特性

  • 1. 西部资源与现代生物技术省部共建教育部重点实验室,西北大学生命科学学院,西安 710069
  • 收稿日期:  2012-03-15
基金项目:

国家自然科学基金资助项目(31000069)

陕西省自然科学基金资助项目(2010JM3012)

陕西省教育厅专项科研计划项目(2010JK853)

摘要: 采用富集培养方法,从多环芳烃污染的土壤中分离筛选到一株能以高分子量的多环芳烃荧蒽为惟一碳源和能源且生长状况良好的菌株DN002。通过形态观察、生理生化指标及16S rRNA同源序列分析比对,结果表明,该菌株为木糖氧化产碱菌(Achromobacter xylosoxidans),最适生长温度为32℃,最适生长pH为7～7.5。该菌株对荧蒽有较强的降解能力,在14 d内对500 mg/L的荧蒽的降解率为92.8%。菌株细胞蛋白SDS-PAGE结果显示,经荧蒽诱导后,在分子量为18～66 kDa范围内有显著的差异蛋白条带。

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