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凹凸棒石粘土固定辣根过氧化物酶处理含酚废水

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鲍腾, 彭书传, 陈冬, 刘海波, 舒林. 凹凸棒石粘土固定辣根过氧化物酶处理含酚废水[J]. 环境工程学报, 2012, 6(9): 3179-3185.
引用本文: 鲍腾, 彭书传, 陈冬, 刘海波, 舒林. 凹凸棒石粘土固定辣根过氧化物酶处理含酚废水[J]. 环境工程学报, 2012, 6(9): 3179-3185.
shu
Bao Teng, Peng Shuchuan, Chen Dong, Liu Haibo, Shu Lin. Horseradish peroxidase immobilization in palygorskite-based material for phenol wastewater treatment[J]. Chinese Journal of Environmental Engineering, 2012, 6(9): 3179-3185.
Citation: Bao Teng, Peng Shuchuan, Chen Dong, Liu Haibo, Shu Lin. Horseradish peroxidase immobilization in palygorskite-based material for phenol wastewater treatment[J]. Chinese Journal of Environmental Engineering, 2012, 6(9): 3179-3185.
shu

凹凸棒石粘土固定辣根过氧化物酶处理含酚废水

  • 1.

    合肥工业大学资源与环境工程学院,合肥 230009

  • 基金项目:

    教育厅安徽高校省级自然科学研究重点项目(KJ2010A060)

    安徽省自然科学基金项目(11040606M99)

    安徽省科技成果转化基金项目(1104b0303023)

  • 中图分类号: X783.4

Horseradish peroxidase immobilization in palygorskite-based material for phenol wastewater treatment

  • 1.

    School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009, China

  • Fund Project:

摘要

  • 摘要: 采用凹凸棒石粘土、可溶性淀粉和工业水玻璃作为制备凹凸棒石粘土基颗粒的材料,并将制备的凹凸棒石粘土基颗粒进行改性,得到了改性后的凹凸棒石基多孔材料,并将其作为固定辣根过氧化物酶的载体,再应用于含酚废水的处理研究,取得了良好的效果。实验结果表明,辣根过氧化物酶的最佳固定化条件为:单位酶活载体量1 mg、固定化时间1.5小时、固定pH值5。并且在固定化酶循环使用6次后,苯酚去除率仍能达到62.3%。
    Abstract: Palygorskite clay, soluble starch and commercial silicate can be used to prepare a type of palygorskite-based granulated material. The granulated material was modified by modified palygorskite-based porous materials. The excellent performance of the porous materials applied in phenol wastewater removal was proved after horseradish peroxidase were fixed on the materials. The experimental results show that 1 mg unit volume of activity vector fixed time of 1.5 hours and pH value of 5 will ensure the best immobilization condition of horseradish peroxidase. After 6 times recycle test of the immobilized enzyme, phenol removal rate can reach 62.3%.
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出版历程
  • 收稿日期:  2012-02-17
  • 刊出日期:  2012-09-14
鲍腾, 彭书传, 陈冬, 刘海波, 舒林. 凹凸棒石粘土固定辣根过氧化物酶处理含酚废水[J]. 环境工程学报, 2012, 6(9): 3179-3185.
引用本文: 鲍腾, 彭书传, 陈冬, 刘海波, 舒林. 凹凸棒石粘土固定辣根过氧化物酶处理含酚废水[J]. 环境工程学报, 2012, 6(9): 3179-3185.
shu
Bao Teng, Peng Shuchuan, Chen Dong, Liu Haibo, Shu Lin. Horseradish peroxidase immobilization in palygorskite-based material for phenol wastewater treatment[J]. Chinese Journal of Environmental Engineering, 2012, 6(9): 3179-3185.
Citation: Bao Teng, Peng Shuchuan, Chen Dong, Liu Haibo, Shu Lin. Horseradish peroxidase immobilization in palygorskite-based material for phenol wastewater treatment[J]. Chinese Journal of Environmental Engineering, 2012, 6(9): 3179-3185.
shu

凹凸棒石粘土固定辣根过氧化物酶处理含酚废水

  • 1. 合肥工业大学资源与环境工程学院,合肥 230009
  • 收稿日期:  2012-02-17
基金项目:

教育厅安徽高校省级自然科学研究重点项目(KJ2010A060)

安徽省自然科学基金项目(11040606M99)

安徽省科技成果转化基金项目(1104b0303023)

摘要: 采用凹凸棒石粘土、可溶性淀粉和工业水玻璃作为制备凹凸棒石粘土基颗粒的材料,并将制备的凹凸棒石粘土基颗粒进行改性,得到了改性后的凹凸棒石基多孔材料,并将其作为固定辣根过氧化物酶的载体,再应用于含酚废水的处理研究,取得了良好的效果。实验结果表明,辣根过氧化物酶的最佳固定化条件为:单位酶活载体量1 mg、固定化时间1.5小时、固定pH值5。并且在固定化酶循环使用6次后,苯酚去除率仍能达到62.3%。

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