紫外诱变选育脱硫微生物
Breed on desulfurization bacteria UV mutagenesis
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摘要: 为了深度脱除石油及其产品中的有机硫，实验从天津大港油田被原油污染的土壤中驯化、分离出对多种硫化合物具有一定脱除能力的枯草芽孢杆菌。以此枯草芽孢杆菌为出发菌株，紫外线为诱变剂进行诱变育种，经过多级水平筛选，并连续进行3次复证后，获得了脱硫效果显著，有效脱硫时间明显缩短的正突变脱硫菌种。该突变菌株对100μg/mLDBT脱硫反应24 h，其平均比脱硫活性由突变前的3.78 mmol/（h?g）提高到21.70 mmol/（h?g），诱变效果显著，并可直接应用于低硫油品的深度脱硫，有一定的实际应用Abstract: A Bacillus subtilis capable of removing sulfur compounds was isolated from the polluted oil in Tianjin Dagang Oilfield. In the experiment，UV-induced desulfurization was used to make Bacillus subtilis get gene mutation，and high-efficient desulfurization Bacillus subtilis of gene mutation was achieved. The desulfurization bacteria for 100μg/mL DBT can increase desulfurization activity from 3.78 mmol/（h?g） to 21.70 mmol/（h?g） in 24 h. They can be applied directly in biodesulfuration of petroleum products，especially for deep desulfurization. They have some actual application value.
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