二鼠李糖脂对白腐菌降解稻草中木质纤维素的影响
Effects of dirhamnolipid on degradation of straw lignocellulose by white rot fungi
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摘要: 通过固态发酵方式采用白腐菌对稻草中木质纤维素进行降解, 并研究了生物表面活性剂二鼠李糖脂对该降解过程的影响。结果表明, 不同浓度的二鼠李糖脂能在不同程度上提高降解过程水溶性有机碳的含量, 添加0.007%和0.021%二鼠李糖脂的实验组最高TOC (total organic carbon)浓度较对照组分别提高了83.6%和54.5%, 这有利于黄孢原毛平革菌的生长, 且延缓了菌体的衰退。添加临界胶束浓度0.007%和0.021%浓度的二鼠李糖脂可使LiP(lignin peroxidase)酶活分别提高85.7%和41.2%, 二鼠李糖脂对MnP(manganese peroxidase)酶活没有显著影响。生物表面活性剂的介入促进了白腐菌对稻草中木质素的降解, 添加0.007%二鼠李糖脂可使木质素降解率提高54%。Abstract: The effect of dirhamnolipid biosurfactant on degradation of straw lignocellulose by white rot fungi in solid state fermentation was studied. The results showed that dirhamnolipid at different concentrations was able to enhance the lignin degradation. Dirhamnolipid at 0.007% and 0.021% (w/w) increased the released water soluble total organic carbon (TOC) by 83.6% and 54.5%, respectively, which provided an opportunity of increasing the growth of Phanerochaete chrysosporium and decreasing its degeneration. In addition, the maximum activity of lignin peroxadase (LiP) was increased by 85.7% with dirhamnolipid at 0.007% (w/w). Whereas, dirhamnolipid have no stimulative effects on manganese peroxidase (MnP) activity. The degradation rate of lignin was increased by 54% with rhamnolipid at 0.007% (w/w) compared to the control.
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Key words:
- biosurfactant /
- dirhamnolipid /
- lignin /
- degradation /
- white rot fungi
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