废水处理系统中活性污泥总DNA的提取

沈国; 李茵

环境工程学报

废水处理系统中活性污泥总DNA的提取

沈国, 李茵. 废水处理系统中活性污泥总DNA的提取[J]. 环境工程学报, 2010, 4(6): 1336-1340.

引用本文:

沈国, 李茵. 废水处理系统中活性污泥总DNA的提取[J]. 环境工程学报, 2010, 4(6): 1336-1340.

Shen Guo, Li Yin. Total DNA extraction from activated sludge in a wastewater biotreatment system[J]. Chinese Journal of Environmental Engineering, 2010, 4(6): 1336-1340.

Citation:

Shen Guo, Li Yin. Total DNA extraction from activated sludge in a wastewater biotreatment system[J]. Chinese Journal of Environmental Engineering, 2010, 4(6): 1336-1340.

废水处理系统中活性污泥总DNA的提取

沈国¹,
李茵¹

1.
东华大学环境科学与工程学院，上海 201620
基金项目:
上海市重点学科建设项目(B604)
中图分类号: X703

Total DNA extraction from activated sludge in a wastewater biotreatment system

Shen Guo¹,
Li Yin¹

1.
College of Environmental Science & Engineering, Donghua University, Shanghai 201620, China
Fund Project:

摘要: 对废水脱氮除磷厌氧-缺氧-好氧系统中的活性污泥样品经过或未经预处理，比较了超声波法、玻璃珠震荡法和冻融法3种细胞裂解方法对DNA提取效果的影响。吸光度和琼脂糖凝胶电泳检测结果表明：活性污泥经TENP和PBS预处理后采用冻融法加2%SDS裂解细胞的DNA提取操作，可以得到数量多、纯度高的活性污泥DNA样品，可不经纯化直接进行PCR扩增反应。
- DNA提取 /
- 细胞裂解 /
- 活性污泥
Abstract: Effects of total DNA extraction from activated sludge with/without pretreatment were compared by three kinds of cell lysis methods, i.e. sonication irradiation, glass bead shaking and freezethawing process in an anaerobic-anoxic-aerobic denitrification-dephosphorization system. By detection of ultraviolet adsorption spectrum analysis and agarose gel electrophoresis, results showed that the DNA samples with high yield and purity were obtained by TENP and PBS pretreated, and 2%SDS and freeze-thawing lyzed, and PCR analysis was done directly without purification.
- DNA extraction /
- cell lysis /
- activated sludge

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沈国, 李茵. 废水处理系统中活性污泥总DNA的提取[J]. 环境工程学报, 2010, 4(6): 1336-1340.

引用本文:

沈国, 李茵. 废水处理系统中活性污泥总DNA的提取[J]. 环境工程学报, 2010, 4(6): 1336-1340.

Shen Guo, Li Yin. Total DNA extraction from activated sludge in a wastewater biotreatment system[J]. Chinese Journal of Environmental Engineering, 2010, 4(6): 1336-1340.

Citation:

Shen Guo, Li Yin. Total DNA extraction from activated sludge in a wastewater biotreatment system[J]. Chinese Journal of Environmental Engineering, 2010, 4(6): 1336-1340.

废水处理系统中活性污泥总DNA的提取

沈国¹,
李茵¹

1. 东华大学环境科学与工程学院，上海 201620

收稿日期: 2009-05-13

基金项目:

上海市重点学科建设项目(B604)

关键词:

DNA提取 /
细胞裂解 /
活性污泥

摘要: 对废水脱氮除磷厌氧-缺氧-好氧系统中的活性污泥样品经过或未经预处理，比较了超声波法、玻璃珠震荡法和冻融法3种细胞裂解方法对DNA提取效果的影响。吸光度和琼脂糖凝胶电泳检测结果表明：活性污泥经TENP和PBS预处理后采用冻融法加2%SDS裂解细胞的DNA提取操作，可以得到数量多、纯度高的活性污泥DNA样品，可不经纯化直接进行PCR扩增反应。

English Abstract

Total DNA extraction from activated sludge in a wastewater biotreatment system

Shen Guo¹,
Li Yin¹

1. College of Environmental Science & Engineering, Donghua University, Shanghai 201620, China

Received Date: 2009-05-13

Fund Project:

Keywords:

Abstract: Effects of total DNA extraction from activated sludge with/without pretreatment were compared by three kinds of cell lysis methods, i.e. sonication irradiation, glass bead shaking and freezethawing process in an anaerobic-anoxic-aerobic denitrification-dephosphorization system. By detection of ultraviolet adsorption spectrum analysis and agarose gel electrophoresis, results showed that the DNA samples with high yield and purity were obtained by TENP and PBS pretreated, and 2%SDS and freeze-thawing lyzed, and PCR analysis was done directly without purification.

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废水处理系统中活性污泥总DNA的提取

Total DNA extraction from activated sludge in a wastewater biotreatment system

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废水处理系统中活性污泥总DNA的提取

English Abstract

Total DNA extraction from activated sludge in a wastewater biotreatment system

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