寒地黑土中阿特拉津降解菌的筛选及降解特性
Screening and identification of atrazine degrading bacteria and their degradation characteristics from black soil in cold area
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摘要: 从长期施用阿特拉津的寒地黑土耕层(0～10 cm)取样。利用富集培养的方法，筛选到2株阿特拉津降解菌，编号Z9和Z42。Z9以阿特拉津为惟一碳氮源生长，Z42以阿特拉津为惟一氮源生长，15 d对阿特拉津的降解率分别为77.7%和65.6%。对其初步鉴定并对降解特性进行研究，结果表明，细菌Z9为微杆菌属（Microbacterium sp.），细菌Z42为节杆菌属(Arthrobacter sp.)。在室内进行降解条件优化实验，得出2株降解菌对100 mg/L阿特拉津的最佳降解条件为：温度30℃,Z9 pH值为7，Z42 pH值为8。Abstract: Two bacteria named Z9 and Z42 were isolated from black soil in cold area (0～10 cm) of long-term applying atrazine by enrichment culture. Z9 grew in medium with atrazine as sole carbon and nitrogen source, Z42 grew in medium with atrazine as sole nitrogen source. After 15 days, the degradation rates of Z9 and Z42 were 77.7% and 65.6% respectively. Based on their physiologic and biochemical characteristics, Z9 and Z42 were identified as Microbacterium sp. and Arthrobacter sp． By studying on degradation characteristics, the optimum degradation condition of two bacteria for 100 mg/L atrazine was established: the temperature was 30℃, pH of Z9 and Z42 were 7 and 8 respectively.
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Key words:
- black soil in cold area /
- atrazine /
- isolation /
- identification /
- degradation characteristics
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