固定化解脂耶氏酵母（Yarrowia lipolytica）处理油脂废水的性能研究
Investigation on capability of disposing grease wastewater with immobilized Yarrowia lipolytica
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摘要: 以海藻酸钠为固定化载体材料，以氯化钙作为交联剂，将高效降解油脂菌——解脂耶氏酵母（Yarrowia lipolytica）包埋制备成固定化微生物小球，用以处理油脂废水，考察了最佳处理条件。结果表明，解脂耶氏酵母经固定化包埋后处理色拉油废水的最适条件为：温度25～35 ℃、pH 4～8、摇床转速100～200 r/min，处理初始油浓度在2 000 mg/L，包埋菌浓度665×106个/mL，包埋量为4 mL/150 L。与悬浮状态相比，固定化微生物温度适应范围增大、热适应性增强、pH值往酸性方向偏移。将固定化解脂耶氏酵母投入YPD液体培养基内驯化30 h再处理色拉油废水，结果显示：驯化后的固定化微生物在同样条件下对色拉油的降解率达到82%，比未驯化状态高20%，且驯化后的机械强度、稳定性和重复使用性都有明显改善。Abstract: The optimal conditions have been studied in disposing grease wastewater with predominant disposing oil bacteria——immobilized Yarrowia lipolytica embedded into microorganisms when using sodium alginate as immobilized carrier and calcium chloride as crosslinker. The results show that the optimal conditions of disposing salad oil for immobilized Yarrowia lipolytica are: temperature 25～30 ℃, pH 4～8, the shaking speed 100～200 r/min, primary oil concentration 2 000 mg/L, embedded bacteria concentration 665×106 entries/mL, embedding quantity 4 mL/150 L. In contrast with suspended bacteria, the embedded microorganisms represent more adaptable capability of a larger range of temperature and an enhancement of heat resistance，a pH avertion to acidity. The result of plunging the immobilized Yarrowia lipolytica into YPD liquid substrate to domesticate for 30 h before disposing salad oil shows that: after domestication, disposing rate of salad oil for the immobilized microorganisms can be 82%, with an increase of 20% compared with the ones that haven't been domesticated with the same condition, furthermore, the mechanical strength，the stability and the repetition for utilization are promoted obviously.
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Key words:
- immobilization /
- sodium alginate /
- Yarrowia lipolytica /
- rease wastewater
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